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puc19-plasmid

Puc19 Plasmid Illustration and Icon

Puc19 Plasmid

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Minimal circular plasmid map representing the pUC19 cloning vector. The ring is divided into three colored sectors (teal, red, yellow) that denote major functional modules typically found in pUC19: a high‑copy pMB1/ColE1 origin of replication, the bla (AmpR) cassette for ampicillin selection, and the lacZα multiple cloning site used for blue‑white screening in Escherichia coli. The figure emphasizes circular dsDNA topology without base‑pair coordinates or labels, using a black outline with teal, red, and yellow segments to indicate feature blocks common to this 2686‑bp vector and its restriction enzyme-rich polylinker (e.g., EcoRI, HindIII, BamHI, PstI, KpnI, XbaI, SalI, SmaI, SacI, SphI).

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