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Paper chromatography 3 Illustration and Icon
Paper chromatography 3
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A horizontal paper strip shows a dark brown origin spot and an adjacent multicolored elution band transitioning from orange-yellow to pale blue, indicating separated solutes migrating with the solvent front. The figure depicts a chromatogram from paper chromatography in the fields of analytical chemistry and biochemistry. Molecules are partitioned between the mobile phase (solvent) and the stationary phase (cellulose paper), producing distinct migration distances quantified by Rf (retention factor). This laboratory technique resolves mixtures of small molecules on a cellulose matrix.
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