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Paper chromatography 1 Illustration and Icon
Paper chromatography 1
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A horizontal chromatogram displays a cellulose paper strip with discrete pigment bands—blue, yellow, light green, and orange—separated along the migration path by a mobile-phase solvent. In the hierarchy of materials, small organic molecules are resolved within a porous paper matrix, an application in analytical chemistry and biochemistry for separating mixtures by polarity and capillary action. The site is the surface of chromatography paper; Rf (retardation factor) values can be inferred from the relative distances traveled by each band.
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