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Oocyte enucleation Illustration and Icon
Oocyte enucleation
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A schematic depicts in vitro micromanipulation for oocyte enucleation in reproductive biology and embryology. A holding pipette stabilizes a mature oocyte, bounded by the zona pellucida (ZP) and perivitelline space (PVS), while a microcapillary aspirates the metaphase II (MII) spindle region near the polar body (PB) to remove maternal chromosomes. The hierarchy shown is the meiotic spindle within a single gamete cell of the female reproductive system. This procedure prepares a cytoplast for somatic cell nuclear transfer (SCNT), an assisted reproductive technology.
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