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Indirect elisa Illustration and Icon
Indirect elisa
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A schematic of an indirect ELISA shows an immobilized antigen bound by a primary antibody (unlabeled Ig; immunoglobulin), which is recognized by an enzyme-conjugated secondary antibody that produces a detectable signal. Within the hierarchy of the immune system, antibodies are soluble proteins that form antigen–antibody complexes on a solid-phase surface in this analytical biochemistry and immunology assay. The method detects and quantifies antigen-specific antibodies in a sample via enzymatic reporter activity that yields a measurable colorimetric or fluorescent readout.
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