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Dead cas9 repression Illustration and Icon
Dead cas9 repression
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Schematic depicting CRISPR interference, where catalytically inactive Cas9 (dCas9, dead CRISPR-associated protein 9) complexed with a single-guide RNA (sgRNA) binds a gene locus on double-stranded DNA to block RNA polymerase (RNAP) and repress transcription. The protein–RNA complex is a macromolecular regulator acting on the genome within a cell. In molecular biology and genetics, this system functions as a programmable transcriptional repressor (CRISPRi) enabling targeted gene silencing.
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