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Crispr cas9 Illustration and Icon
Crispr cas9
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Illustration depicts CRISPR-Cas9-mediated genome editing, with an RNA-guided nuclease cutting target double-stranded DNA. A guide RNA (gRNA; guide ribonucleic acid) base-pairs with the complementary genomic sequence, positioning the CRISPR associated protein 9 (Cas9) to introduce a site-specific double-strand break (DSB). This ribonucleoprotein complex represents a protein–RNA molecular tool within the hierarchy of macromolecules acting on chromosomal DNA in cells. In molecular biology and genetics, it enables targeted gene disruption or repair via non-homologous end joining or homology-directed repair.
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