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Crispr cas9 nhej Illustration and Icon
Crispr cas9 nhej
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A schematic illustrates CRISPR-Cas9–mediated genome editing in which a guide RNA (gRNA) directs the Cas9 endonuclease to introduce a double-strand break (DSB) in genomic DNA, followed by repair via non-homologous end joining (NHEJ). In biological hierarchy, a DNA molecule within a chromosome of a cell is targeted. In molecular biology and genetics, this process is applied to disrupt gene function as error-prone NHEJ generates insertions or deletions (indels). The depicted entities include a protein nuclease, RNA guide, and the DNA substrate.
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