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Crispr cas9 nhej gene disruption Illustration and Icon
Crispr cas9 nhej gene disruption
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A schematic illustrates targeted genome editing in which CRISPR–Cas9 creates a double-strand break (DSB) in genomic DNA, and error-prone non-homologous end joining (NHEJ) repairs the break, generating an insertion/deletion (indel) depicted in red that disrupts a coding sequence. In the biological hierarchy, a gene within a chromosome is shown as part of the cellular genome. The image pertains to molecular genetics and genome engineering, highlighting an approach used for gene knockout and loss-of-function studies. CRISPR stands for clustered regularly interspaced short palindromic repeats; Cas9 is the CRISPR-associated protein 9.
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