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Crispr cas9 hdr insertion Illustration and Icon
Crispr cas9 hdr insertion
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A schematic depicts targeted genome editing in which Cas9 introduces a double-strand break (DSB) in chromosomal DNA, followed by homology-directed repair (HDR) to insert a donor sequence (red). The diagram shows a DNA locus (black) with homology arms (blue) flanking the insertion. In the biological hierarchy, the DNA molecule resides within a nuclear chromosome of a cell. Relevant fields include molecular biology and genetics. The system uses CRISPR (clustered regularly interspaced short palindromic repeats), Cas9 (CRISPR-associated protein 9), and a guide RNA (gRNA).
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