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Primary cardiomyocyte isolation

Fabricio Pamplona

  • Slide 1

KEYWORDS

petri dish, laboratory, labware, glassware, dish, pipette, equipment, lab, lab equipment, lab procedure, procedure, pipetting, vial, microtube, tube, plasticware, eppendorf, body, mouse, mouse strain, strain, lab animal, laboratory mouse, laboratory animal, rodent, non-isogenic mouse, non-isogenic strain, outbred mouse, Swiss, Swiss albino, CD1

ABOUT

Cultured primary cardiomyocytes are widely used to study and understand the mechanisms underlying normal cardiovascular function, cardioprotection, and cardiovascular diseases. Cardiomyocyte cultures provide a homogeneous population of single cells, which are easy to visualize and manipulate. Compared with the whole heart, cardiomyocyte cultures are relatively pure, with limited contaminating cell types such as endothelial cells. In addition, preparations of heart cells isolated from small mammals like mouse and rat enable a large number of quick, relatively low-cost experiments compared to studies conducted in whole animals. Source: https://www.thermofisher.com/kr/ko/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/protein-biology-application-notes/one-hour-procedure-isolate-primary-cardiomyocytes-neonatal-mouse-rat-hearts.html

AUTHOR

Fabricio Pamplona

Pharmacist

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