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Sandwich elisa Illustration and Icon
Sandwich elisa
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Schematic diagram of a sandwich enzyme-linked immunosorbent assay (ELISA), depicting a plate-immobilized capture antibody (Ig) binding an antigen, which is recognized by a secondary detection antibody conjugated to an enzyme reporter that produces a colorimetric signal. At the hierarchy level, individual proteins (antibodies and antigen) assemble into an immunocomplex on the surface of a microtiter plate well within an analytical immunology/biochemistry assay. The method enables sensitive detection and quantification of soluble biomarkers in clinical and research samples.
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