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dead-cas9-activation

Dead cas9 activation Illustration and Icon

Dead cas9 activation

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A stylized molecular diagram depicts a catalytically inactive Cas9 bound to DNA and guided by an sgRNA to enhance transcription at a target locus. The ribonucleoprotein complex (dCas9, deactivated CRISPR-associated protein 9, plus sgRNA, single-guide RNA) binds chromosomal DNA near a gene, illustrating a hierarchy of protein–RNA complex acting on nucleic acid within the genome. In molecular biology and synthetic biology, this system (CRISPRa, CRISPR activation) recruits transcriptional machinery to increase gene expression without generating double-strand breaks.

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