{"id":12979,"date":"2021-06-08T20:10:05","date_gmt":"2021-06-08T23:10:05","guid":{"rendered":"https:\/\/mindthegraph.com\/blog\/?p=12979"},"modified":"2023-01-05T14:22:32","modified_gmt":"2023-01-05T17:22:32","slug":"microscopy-how-scientists-use-fluorescence-to-see-inside-cells","status":"publish","type":"post","link":"https:\/\/mindthegraph.com\/blog\/zh\/%e6%98%be%e5%be%ae%e9%95%9c-%e7%a7%91%e5%ad%a6%e5%ae%b6%e5%a6%82%e4%bd%95%e5%88%a9%e7%94%a8%e8%8d%a7%e5%85%89%e8%a7%82%e5%af%9f%e7%bb%86%e8%83%9e%e5%86%85%e9%83%a8%e7%9a%84%e6%83%85%e5%86%b5\/","title":{"rendered":"\u663e\u5fae\u955c\u3002\u79d1\u5b66\u5bb6\u4eec\u5982\u4f55\u5229\u7528\u8367\u5149\u6765\u89c2\u5bdf\u7ec6\u80de\u5185\u90e8"},"content":{"rendered":"<p>\u79d1\u5b66\u5bb6\u7684\u65e5\u5e38\u5de5\u4f5c\u7684\u5f88\u5927\u4e00\u90e8\u5206\u662f\u8bbe\u8ba1\u548c\u8fdb\u884c\u5b9e\u9a8c\u3002<\/p>\n\n\n\n<p>\u5b9e\u9a8c\u5ba4\u6280\u672f\u7684\u7ec4\u5408\u5c06\u56de\u7b54\u79d1\u5b66\u5bb6\u63d0\u51fa\u7684\u5927\u90e8\u5206\u95ee\u9898\uff0c\u800c\u63d0\u51fa\u65b0\u65b9\u6cd5\u7684\u5de5\u4f5c\u6d41\u7a0b\u53d6\u51b3\u4e8e\u79d1\u5b66\u5bb6\u7684\u80cc\u666f\u548c\u7ecf\u9a8c\u3002<\/p>\n\n\n\n<p>\u5bf9\u4e8e\u751f\u7269\u5b66\u5bb6\u6765\u8bf4\uff0c\u7ec6\u80de\u56fe\u50cf\u53ef\u4ee5\u8bf4\u660e\u4ed6\u4eec\u6b63\u5728\u7814\u7a76\u7684\u8fc7\u7a0b\u548c\u673a\u5236\u53d1\u751f\u4e86\u4ec0\u4e48\u3002<\/p>\n\n\n\n<p 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<a href=\"https:\/\/link.springer.com\/article\/10.1007\/s13346-019-00657-8\">\u95e8\u4e1c\u8428\u7684<\/a> 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class=\"wp-block-image\">\n<figure class=\"aligncenter size-large\"><a href=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/06\/preview-347880-7.png\"><img decoding=\"async\" loading=\"lazy\" width=\"788\" height=\"1024\" src=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/06\/preview-347880-7-788x1024.png\" alt=\"Mind the Graph\u4fe1\u606f\u56fe\u5982\u4f55\u8bbe\u8ba1\u514d\u75ab\u8367\u5149\u7684\u9762\u677f\" class=\"wp-image-12980\" srcset=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/06\/preview-347880-7-788x1024.png 788w, https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/06\/preview-347880-7-231x300.png 231w, https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/06\/preview-347880-7-768x998.png 768w, https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/06\/preview-347880-7-1182x1536.png 1182w, https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/06\/preview-347880-7-1575x2048.png 1575w, https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/06\/preview-347880-7-9x12.png 9w, https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/06\/preview-347880-7-77x100.png 77w\" sizes=\"(max-width: 788px) 100vw, 788px\" \/><\/a><\/figure><\/div>\n\n\n<p>\u8ba9\u6211\u4eec\u6765\u4e86\u89e3RECA-1\uff08\u7ea2\u8272\uff09\u7684\u6297\u4f53\u5728\u505a\u4ec0\u4e48\u3002<\/p>\n\n\n\n<p>\u8fd9\u4e9b\u6297\u4f53\u88ab\u8bbe\u8ba1\u4e3a\u7279\u5b9a\u7684\u63a2\u9488\uff0c\u5b83\u4eec\u9488\u5bf9\u7279\u5b9a\u7684\u6297\u539f\uff08\u5728\u6211\u4eec\u7684\u4f8b\u5b50\u4e2d\u662f\u86cb\u767d\u8d28RECA-1\uff09\u3002<\/p>\n\n\n\n<p>\u5b83\u4eec\u53ef\u4ee5\u7528\u8367\u5149\u4f53\u8fdb\u884c\u6807\u8bb0\uff0c\u6216\u5728\u4e4b\u540e\u88ab\u4e0e\u8367\u5149\u4f53\u76f8\u8fde\u7684\u7b2c\u4e8c\u6297\u4f53\u8bc6\u522b\u3002<\/p>\n\n\n\n<p>\u56e0\u6b64\uff0c\u5728\u7528\u5149\u6e90\u523a\u6fc0\u6837\u54c1\u540e\u3002 <a href=\"https:\/\/onlinelibrary.wiley.com\/doi\/abs\/10.1002\/jobm.3620300304\">\u4f60\u8981\u5bfb\u627e\u7684\u7279\u5b9a\u86cb\u767d\u8d28\u5c06\u901a\u8fc7\u53d1\u5c04\u7279\u5b9a\u6ce2\u957f\u7684\u5149\u5728\u4f60\u7684\u6837\u54c1\u4e2d\u88ab\u8bc6\u522b\u3002<\/a>.<\/p>\n\n\n\n<p>\u5c31DAPI\u800c\u8a00\uff0c\u8fd9\u79cd\u67d3\u6599\u662f\u6838\u5b50\u548c\u6838\u7cd6\u4f53\u7684\u53cd\u67d3\u8272\u5242\uff0c\u5b83\u4e0eDNA\u7684AT\u533a\u57df\u7ed3\u5408\u65f6\u53d1\u51fa\u84dd\u8272\u8367\u5149\u3002<\/p>\n\n\n\n<p class=\"has-large-font-size\"><strong>\u5982\u4f55\u8bbe\u8ba1\u514d\u75ab\u8367\u5149\u7684\u9762\u677f\uff1f <\/strong><\/p>\n\n\n\n<p class=\"has-large-font-size\"><strong>\u4ece\u8fd9\u4e9b\u6b65\u9aa4\u5f00\u59cb\u3002<\/strong><\/p>\n\n\n\n<ol>\n<li>\u8d2d\u4e70\uff08\u6216\u501f\u7528\uff01\u79d1\u5b66\u5e94\u8be5\u662f\u975e\u5e38\u5408\u4f5c\u7684\uff01\uff09\u4f60\u7684\u7814\u7a76\u4e2d\u5fc5\u4e0d\u53ef\u5c11\u7684\u6297\u4f53\u548c\u67d3\u6599\u3002\u4f18\u5148\u9009\u62e9\u4e00\u7ea7\u6297\u4f53\uff08\u4e0d\u542b\u63a2\u9488\uff09\uff0c\u5e76\u4ece\u4e00\u7ea7\u6297\u4f53\u4e2d\u8d2d\u4e70\u9488\u5bf9\u5bbf\u4e3b\u7269\u79cd\u7684\u4e8c\u7ea7\u6297\u4f53\u3002\u4f8b\u5982\uff0c\u5982\u679c\u4f7f\u7528\u5728\u5154\u5b50\u8eab\u4e0a\u4ea7\u751f\u7684\u521d\u7ea7\u6297\u4f53\uff0c\u4f7f\u7528\u6297\u5154\u5b50\u7684\u4e8c\u7ea7\u6297\u4f53\u3002\u8fd9\u5c06\u4fdd\u8bc1\u7279\u5f02\u6027\u3002&nbsp;<\/li>\n\n\n\n<li>\u4f7f\u7528\u7528\u8367\u5149\u56e2\u6807\u8bb0\u7684\u4e8c\u7ea7\u6297\u4f53\u5c06\u901a\u8fc7\u68c0\u6d4b\u6bcf\u4e2a\u4e00\u7ea7\u6297\u4f53\u7684\u66f4\u591a\u6297\u539f\u6765\u589e\u5f3a\u4f60\u7684\u4fe1\u53f7\u3002\u53e6\u5916\uff0c\u8fd9\u662f\u4e00\u79cd\u66f4\u6709\u6d3b\u529b\u7684\u65b9\u5f0f\u6765\u9610\u8ff0\u4e0d\u540c\u7684\u68c0\u6d4b\uff0c\u56e0\u4e3a\u5b83\u5141\u8bb8\u7814\u7a76\u4eba\u5458\u6839\u636e\u5979\u7684\u9700\u8981\u6765\u4fee\u6539\u9762\u677f\u4e2d\u7684\u989c\u8272\u3002&nbsp;<\/li>\n\n\n\n<li>\u53e6\u4e00\u4e2a\u91cd\u8981\u6b65\u9aa4\u662f\u5728\u4f60\u7684\u663e\u5fae\u955c\u5185\u68c0\u67e5\u6709\u54ea\u4e9b\u6ee4\u5149\u7247\u53ef\u4f9b\u4f7f\u7528\u3002\u4f60\u5e94\u8be5\u786e\u4fdd\u4f60\u7684\u8367\u5149\u4f53\u6fc0\u53d1\u548c\u53d1\u5c04\u6ce2\u957f\u4f4d\u4e8e\u6fc0\u53d1\u548c\u53d1\u5c04\u6ee4\u5149\u7247\u5185\uff1b\u5426\u5219\uff0c\u4f60\u5c06\u65e0\u6cd5\u6355\u6349\u5230\u63a2\u9488\u7684\u53d1\u5c04\u5149\u3002\u4f60\u53ef\u4ee5\u4f7f\u7528 <a href=\"https:\/\/www.thermofisher.com\/order\/spectra-viewer\">\u8367\u5149\u5149\u8c31\u67e5\u770b\u5668<\/a> \u6765\u68c0\u67e5\u517c\u5bb9\u6027\u3002<\/li>\n\n\n\n<li>\u8981\u786e\u4fdd\u4f60\u6240\u6709\u7684\u8367\u5149\u4f53\u548c\u67d3\u6599\u7684\u6fc0\u53d1\u548c\u53d1\u5c04\u6ce2\u957f\u4e0d\u4f1a\u5728\u540c\u4e00\u4e2a\u5b9e\u9a8c\u91cc\u9762\u91cd\u53e0\u3002 <a href=\"https:\/\/www.thermofisher.com\/order\/spectra-viewer\">\u8367\u5149\u5149\u8c31\u67e5\u770b\u5668<\/a> \u662f\u4e00\u4e2a\u5f88\u597d\u7684\u9009\u62e9\u3002\u5b83\u4eec\u51e0\u4e4e\u6db5\u76d6\u4e86\u6240\u6709\u53ef\u7528\u7684\u8367\u5149\u4f53!<\/li>\n<\/ol>\n\n\n\n<p>\u6700\u540e\uff0c\u68c0\u67e5\u4e00\u4e2a\u5047\u8bbe\u5b9e\u9a8c\u7684\u4f8b\u5b50\uff0c\u6211\u4eec\u7528Hoechst 33258\u8868\u793a\u6838\u9178\uff0c\u7528\u4e8c\u7ea7\u6297\u4f53Alexa Fluor 647\u6807\u8bb0\u7684RECA-1\u7684\u4e00\u7ea7\u6297\u4f53\u3002<\/p>\n\n\n\n<p>\u7406\u60f3\u60c5\u51b5\u4e0b\uff0c\u6211\u4eec\u4f1a\u4f7f\u7528\u4e00\u4e2a\u53ef\u63d0\u4f9bDAPI\u7acb\u65b9\u4f53\uff08\u6fc0\u53d1377\/50\u548c\u53d1\u5c04447\/60\uff09\uff0c\u548cCY5\u7acb\u65b9\u4f53\uff08\u6fc0\u53d1628\/40\u548c\u53d1\u5c04685\/40\uff09\u7684\u663e\u5fae\u955c\u3002\u6240\u6709\u8fd9\u4e9b\u4fe1\u606f\u6211\u4eec\u90fd\u63d2\u5728 <a href=\"https:\/\/www.thermofisher.com\/order\/spectra-viewer\">\u8367\u5149\u5149\u8c31\u67e5\u770b\u5668<\/a> \u5e76\u83b7\u5f97\u4e86\u4e24\u79cd\u67d3\u6599\u7684\u5149\u8c31\uff0c\u4ee5\u53ca\u4e24\u79cd\u7acb\u65b9\u4f53\u7684\u5e26\u5bbd\uff08\u67e5\u770b\u4e0a\u9762\u4fe1\u606f\u56fe\u4e2d\u7684\u5149\u8c31\uff09\u3002<\/p>\n\n\n\n<p>\u8fd9\u7bc7\u5047\u8bbe\u7684\u6587\u7ae0\u662f\u4e00\u4e2a\u5f88\u597d\u7684\u4f8b\u5b50\uff0c\u8367\u5149\u4f53\u7684\u5149\u8c31\u5c5e\u4e8e\u6fc0\u53d1\u548c\u53d1\u5c04\u6ee4\u5149\u7247\u5185\uff0c\u4f7f\u7814\u7a76\u4eba\u5458\u6709\u53ef\u80fd\u4ee5\u6700\u4f73\u65b9\u5f0f\u6355\u6349\u4ed6\u7684\u6837\u672c\u3002<\/p>\n\n\n\n<p>\u73b0\u5728\uff0c\u662f\u65f6\u5019\u53bb\u5b9e\u9a8c\u5ba4\u5c06\u4e00\u5207\u4ed8\u8bf8\u5b9e\u8df5\u4e86\uff01\"\u3002<\/p>\n\n\n\n<p>\u6211\u5e0c\u671b\u8fd9\u4e9b\u63d0\u793a\u5bf9\u4f60\u7684\u4e0b\u4e00\u4e2a\u5b9e\u9a8c\u6709\u6240\u5e2e\u52a9\u3002\u795d\u60a8\u597d\u8fd0!<\/p>\n\n\n\n<p><strong>\u53c2\u8003\u6587\u732e\u3002<\/strong><\/p>\n\n\n\n<ol>\n<li><a href=\"https:\/\/www.zotero.org\/google-docs\/?imatmT\">\u8367\u5149\u663e\u5fae\u955c\u7b80\u4ecb\u3002 <em>\u5c3c\u5eb7\u7684MicroscopyU <\/em><\/a><a href=\"https:\/\/www.microscopyu.com\/techniques\/fluorescence\/introduction-to-fluorescence-microscopy\">https:\/\/www.microscopyu.com\/techniques\/fluorescence\/introduction-to-fluorescence-microscopy<\/a><a href=\"https:\/\/www.zotero.org\/google-docs\/?imatmT\">.2021-04-11 17:20:40\u8bbf\u95ee\u3002<\/a><\/li>\n<\/ol>","protected":false},"excerpt":{"rendered":"<p>\u79d1\u5b66\u5bb6\u7684\u65e5\u5e38\u5de5\u4f5c\u7684\u5f88\u5927\u4e00\u90e8\u5206\u662f\u8bbe\u8ba1\u548c\u6267\u884c\u5b9e\u9a8c\u3002\u5b9e\u9a8c\u6280\u672f\u7684\u7ec4\u5408\u5c06\u56de\u7b54\u79d1\u5b66\u5bb6\u63d0\u51fa\u7684\u5927\u90e8\u5206\u95ee\u9898\uff0c\u800c\u63d0\u51fa\u65b0\u65b9\u6cd5\u7684\u5de5\u4f5c\u6d41\u7a0b\u53d6\u51b3\u4e8e\u79d1\u5b66\u5bb6\u7684\u80cc\u666f\u548c\u7ecf\u9a8c\u3002\u5bf9\u4e8e\u751f\u7269\u5b66\u5bb6\u6765\u8bf4\uff0c\u7ec6\u80de\u56fe\u50cf\u53ef\u4ee5\u8bf4\u660e\u5f88\u591a\u5173\u4e8e[...]\u7684\u60c5\u51b5\u3002<\/p>","protected":false},"author":18,"featured_media":12982,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[66,959,958,28,38],"tags":[51,554,962],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v19.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Microscopy: How Scientists Use Fluorescence To See Inside Cells -<\/title>\n<meta name=\"description\" content=\"Fluorescence microscopy can say a lot about a cell process through image. That is why knowing how to design the experiment is so important.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mindthegraph.com\/blog\/zh\/\u663e\u5fae\u955c-\u79d1\u5b66\u5bb6\u5982\u4f55\u5229\u7528\u8367\u5149\u89c2\u5bdf\u7ec6\u80de\u5185\u90e8\u7684\u60c5\u51b5\/\" \/>\n<meta property=\"og:locale\" content=\"zh_CN\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Microscopy: How Scientists Use Fluorescence To See Inside Cells\" \/>\n<meta property=\"og:description\" content=\"Fluorescence microscopy can say a lot about a cell process through image. That is why knowing how to design the experiment is so important.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/mindthegraph.com\/blog\/zh\/\u663e\u5fae\u955c-\u79d1\u5b66\u5bb6\u5982\u4f55\u5229\u7528\u8367\u5149\u89c2\u5bdf\u7ec6\u80de\u5185\u90e8\u7684\u60c5\u51b5\/\" \/>\n<meta property=\"og:site_name\" content=\"Mind the Graph Blog\" \/>\n<meta property=\"article:published_time\" content=\"2021-06-08T23:10:05+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2023-01-05T17:22:32+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/06\/microscopy-facebook.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1200\" \/>\n\t<meta property=\"og:image:height\" content=\"628\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Bruna Soldera\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:title\" content=\"Microscopy: How Scientists Use Fluorescence To See Inside Cells\" \/>\n<meta name=\"twitter:image\" content=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/06\/microscopy-twitter.jpg\" \/>\n<meta name=\"twitter:label1\" content=\"\u4f5c\u8005\" \/>\n\t<meta name=\"twitter:data1\" content=\"Bruna Soldera\" \/>\n\t<meta name=\"twitter:label2\" content=\"\u9884\u8ba1\u9605\u8bfb\u65f6\u95f4\" \/>\n\t<meta name=\"twitter:data2\" content=\"5\u5206\" \/>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Microscopy: How Scientists Use Fluorescence To See Inside Cells -","description":"Fluorescence microscopy can say a lot about a cell process through image. 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