{"id":11803,"date":"2020-10-28T09:30:35","date_gmt":"2020-10-28T11:30:35","guid":{"rendered":"https:\/\/mindthegraph.com\/blog\/?p=11803"},"modified":"2022-10-18T08:09:32","modified_gmt":"2022-10-18T11:09:32","slug":"rt-pcr-test-for-coronavirus","status":"publish","type":"post","link":"https:\/\/mindthegraph.com\/blog\/zh\/rt-pcr-test-for-coronavirus\/","title":{"rendered":"\u51a0\u72b6\u75c5\u6bd2\u7684RT-PCR\u6d4b\u8bd5"},"content":{"rendered":"<p><span style=\"font-weight: 400;\">\u4ece2020\u5e74\u4f60\u6240\u542c\u5230\u7684\u5173\u4e8eCOVID-19\u7684\u8bb8\u591a\u4e8b\u60c5\u4e2d\uff0c\u6709\u4e00\u4ef6\u4e8b\u662f\u7528\u6765\u68c0\u6d4b\u67d0\u4eba\u662f\u5426\u88ab\u75c5\u6bd2\u6c61\u67d3\u7684\u6d4b\u8bd5\uff0c\u5373\u8457\u540d\u7684RT-PCR\u6d4b\u8bd5\u6216\u4ec5\u662fPCR\u3002<\/span><\/p>\n<p><span style=\"font-weight: 400;\">\u5f53\u7136\uff0c\u4f60\u4ee5\u524d\u5df2\u7ecf\u4ece\u533b\u7597\u4e13\u4e1a\u4eba\u5458\u6216\u65b0\u95fb\u4e2d\u542c\u8bf4\u8fc7\u8fd9\u79cd\u6d4b\u8bd5\u3002PCR\u6d4b\u8bd5\u88ab\u7528\u6765\u68c0\u6d4b\u67d0\u4eba\u4f53\u5185\u662f\u5426\u5b58\u5728\u75c5\u6bd2\u3002<\/span><\/p>\n<p><span style=\"font-weight: 400;\">RT-PCR\u68c0\u6d4b\u4e0d\u662f\u552f\u4e00\u53ef\u4ee5\u68c0\u6d4b\u75c5\u6bd2\u7684\u65b9\u6cd5\uff0c\u4f46\u4e0e\u5176\u4ed6\u65b9\u6cd5\u76f8\u6bd4\uff0c\u5b83\u662f\u6700\u51c6\u786e\u548c\u6700\u53ef\u9760\u7684\u65b9\u6cd5\u3002\u53e6\u4e00\u79cd\u6d4b\u8bd5\u662f\u4f20\u7edf\u7684\u8840\u6e05\u5b66\u6d4b\u8bd5\uff0c\u68c0\u6d4b\u8840\u6db2\u6837\u672c\u4e2d\u7684\u7279\u5b9a\u6297\u4f53\u6c34\u5e73\u3002<\/span><\/p>\n<p><span style=\"font-weight: 400;\">RT-PCR\u4e2d\u7684\u5b57\u6bcd\uff0c\u4ee3\u8868\u4e86 <\/span><b>\u53cd\u8f6c\u5f55\u805a\u5408\u9176\u94fe\u53cd\u5e94<\/b><span style=\"font-weight: 400;\">\u5728\u8fc7\u53bb\u7684\u51e0\u5341\u5e74\u91cc\uff0c\u79d1\u5b66\u5bb6\u4eec\u4e00\u76f4\u5728\u4f7f\u7528\u5b83\u6765\u751f\u4ea7\u591a\u4e2a\u9057\u4f20\u7269\u8d28\u7684\u526f\u672c\u3002<\/span><\/p>\n<p><span style=\"font-weight: 400;\">\u6362\u53e5\u8bdd\u8bf4\uff0c\u5728\u8fd9\u79cd\u60c5\u51b5\u4e0b\uff0c\u66f4\u591a\u6765\u81eaSARS-CoV-2\u7684\u9057\u4f20\u7269\u8d28\uff0c\u5b83\u4eec\u88ab\u7528\u4f5c\u6307\u7eb9\uff0c\u4ee5\u8bc1\u660e\u4ece\u75c5\u4eba\u7684\u9f3b\u5b50\u3001\u8033\u6735\u6216\u553e\u6db2\u6536\u96c6\u7684\u6837\u672c\u4e2d\u5b58\u5728\u8be5\u75c5\u6bd2\u3002\u5982\u679c\u6709\u4efb\u4f55\u75c5\u6bd2\u9690\u85cf\u5728\u6837\u54c1\u4e2d\uff0c\u5373\u4f7f\u662f\u5c11\u91cf\u7684\uff0c\u5b83\u4e5f\u80fd\u68c0\u6d4b\u51fa\u6765\uff0c\u56e0\u4e3a\u5b83\u7684\u590d\u5236-\u7c98\u8d34\u5faa\u73af\u3002\u8fd9\u5c31\u662f\u4e3a\u4ec0\u4e48RT-PCR\u6d4b\u8bd5\u5982\u6b64\u53ef\u9760\u3002<\/span><\/p>\n<p><b>\u6d4b\u8bd5<\/b><span style=\"font-weight: 400;\"> \u662f\u4e00\u79cd\u57fa\u672c\u4e0a\u5728\u6e29\u5ea6\u5468\u671f\u5185\u6709\u4e24\u4e2a\u6b65\u9aa4\u7684\u65b9\u6cd5\uff0c\u5b83\u5bf9\u4e00\u5757\u9057\u4f20\u7269\u8d28\uff0c\u65e0\u8bba\u662fDNA\u8fd8\u662fRNA\uff0c\u8fdb\u884c\u590d\u5236-\u7c98\u8d34\u7684\u8fc7\u7a0b\u3002<\/span><\/p>\n<p><span style=\"font-weight: 400;\">\u8be5\u6d4b\u8bd5\u9700\u8981\u4e00\u4e2a <\/span><b>\u70ed\u5faa\u73af\u4eea<\/b><span style=\"font-weight: 400;\"> \u5176\u4e2d\u6709\u4e00\u4e2a\u7cbe\u786e\u7684 <\/span><b>\u6e29\u5ea6\u548c\u65f6\u95f4\u63a7\u5236<\/b><span style=\"font-weight: 400;\">.\u901a\u8fc7\u591a\u4e2a\u6e29\u5ea6\u5faa\u73af\uff0c\u75c5\u6bd2\u7684\u9057\u4f20\u7269\u8d28\u4f1a\u589e\u52a0\u3002\u4e0b\u56fe\u662f\u4e00\u4e2a\u6e29\u5ea6\u5faa\u73af\u7684\u4f8b\u5b50\u3002<\/span><\/p>\n<p><a href=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2020\/10\/polymerase-chain-reaction-steps.png\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-11804 size-full\" src=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2020\/10\/polymerase-chain-reaction-steps.png\" alt=\"\" width=\"567\" height=\"391\" srcset=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2020\/10\/polymerase-chain-reaction-steps.png 567w, https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2020\/10\/polymerase-chain-reaction-steps-300x207.png 300w\" sizes=\"(max-width: 567px) 100vw, 567px\" \/><\/a><\/p>\n<p><span style=\"font-weight: 400;\">\u8be5\u65b9\u6cd5\u9700\u8981\u4e24\u79cd\u4e3b\u8981\u7684\u9176\uff0c\u5373\u9006\u8f6c\u5f55\u9176\uff08\u8fd9\u5c31\u662f\u6807\u9898\u4e2d \"RT \"\u7684\u539f\u56e0\uff09\u548cDNA\u805a\u5408\u9176\u3002\u5b83\u4eec\u4e2d\u7684\u6bcf\u4e00\u4e2a\u90fd\u6709\u4e00\u4e2a\u4f5c\u7528\u9636\u6bb5\u548c\u4e00\u4e2a\u529f\u80fd\u3002\u5728\u4e0a\u56fe\u4e2d\uff0c\u5468\u671f\u7684\u9ec4\u8272\u9636\u6bb5\u662fRT\u53d1\u6325\u4f5c\u7528\u7684\u65f6\u5019\uff0c\u7ea2\u8272\u9636\u6bb5\u662fDNA\u805a\u5408\u9176\u5b8c\u6210\u5176\u5de5\u4f5c\u7684\u65f6\u5019\u3002<\/span><\/p>\n<p><span style=\"font-weight: 400;\">\u7b2c\u4e00\u79cd\u9176\u4eceRNA\u75c5\u6bd2\u4e2d\u5236\u9020\u51fa\u4e92\u8865\u7684DNA\u94fe\uff0c\u4f7f\u7b2c\u4e8c\u79cd\u9176\u80fd\u591f\u4ece\u65b0\u5236\u9020\u7684DNA\u94fe\u4e0a\u8fdb\u884c\u590d\u5236\u7c98\u8d34\uff0c\u589e\u52a0\u9057\u4f20\u7269\u8d28\u7684\u6570\u91cf\u3002\u8fd9\u79cd\u6269\u589e\u4f7f\u5f97\u63cf\u8ff0\u548c\/\u6216\u64cd\u7eb5\u9057\u4f20\u7269\u8d28\u6210\u4e3a\u53ef\u80fd\uff0c\u800c\u5728\u8f83\u5c0f\u7684\u6570\u91cf\u4e0b\u662f\u4e0d\u53ef\u80fd\u7684\u3002<\/span><\/p>\n<p><a href=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2020\/10\/dna-polyemrase.png\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-11806 size-full\" src=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2020\/10\/dna-polyemrase.png\" alt=\"\" width=\"375\" height=\"154\" srcset=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2020\/10\/dna-polyemrase.png 375w, https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2020\/10\/dna-polyemrase-300x123.png 300w\" sizes=\"(max-width: 375px) 100vw, 375px\" \/><\/a><\/p>\n<p><span style=\"font-weight: 400;\">\u6240\u6709\u8fd9\u4e9b\u8fc7\u7a0b\u7684\u53d1\u751f\u90fd\u9700\u8981\u4e00\u79cd\u53eb\u505a\u5f15\u7269\uff08PRIMER\uff09\u7684\u5c0f\uff08\u6216\u5927\uff09\u5206\u5b50\u7684\u5e2e\u52a9\u3002\u5f15\u7269\u7684\u4f5c\u7528\u662f\u6307\u793a\u9176\u5e94\u8be5\u5f00\u59cb\u5ef6\u4f38\u94fe\u7684\u4f4d\u7f6e\uff0c\u6ca1\u6709\u5b83\uff0cDNA\u805a\u5408\u9176\u7684\u590d\u5236-\u7c98\u8d34\u8fc7\u7a0b\u5c31\u4e0d\u4f1a\u5f00\u59cb\u3002<\/span><\/p>\n<p><span style=\"font-weight: 400;\">\u5f15\u7269\u7684\u9009\u62e9\u53ef\u4ee5\u662f\u968f\u673a\u7684\uff0c\u4e5f\u53ef\u4ee5\u662f\u7279\u5b9a\u7684\u5f62\u72b6\u3002\u5728\u8fd9\u79cd\u60c5\u51b5\u4e0b\uff0c\u4e86\u89e3V\u00edrus\u7684\u9057\u4f20\u56fe\u8c31\u662f\u975e\u5e38\u91cd\u8981\u7684\uff0c\u8fd9\u6837\u53ef\u4ee5\u4f7f\u7528\u7279\u5b9a\u7684\u5f15\u7269\u6765\u63d0\u9ad8\u7ed3\u679c\u7684\u6548\u7387\u548c\u53ef\u9760\u6027\u3002<\/span><\/p>\n<p><span style=\"font-weight: 400;\">\u5982\u679c\u4f60\u50cf\u6211\u4eec\u4e00\u6837\u597d\u5947\uff0c\u60f3\u77e5\u9053\u5173\u4e8eRT-PCR\u7684\u66f4\u591a\u7ec6\u8282\uff0c\u4f60\u53ef\u4ee5\u67e5\u770b\u66f4\u591a\u7ec6\u8282\u00a0<\/span><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/14667534\/\"><span style=\"font-weight: 400;\"> \u8fd9\u7bc7\u79d1\u5b66\u6587\u7ae0 <\/span><span style=\"font-weight: 400;\">\u5c31\u5728\u8fd9\u91cc<\/span><\/a><span style=\"font-weight: 400;\">\u00a0 \u4f46\u5b9e\u8d28\u4e0a\uff0c\u8fd9\u91cc\u6709\u505a\u6d4b\u8bd5\u6240\u9700\u7684\u4e00\u5207\u3002<\/span><\/p>\n<p><span style=\"color: #000000;\"><strong>\u75c5\u6bd2\u7684\u9057\u4f20\u7269\u8d28\uff0c\u5373RNA<\/strong><\/span><\/p>\n<p><a href=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2020\/10\/viral-genetic-material-the-rna.png\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-11808 size-full\" src=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2020\/10\/viral-genetic-material-the-rna.png\" alt=\"\" width=\"370\" height=\"157\" srcset=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2020\/10\/viral-genetic-material-the-rna.png 370w, https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2020\/10\/viral-genetic-material-the-rna-300x127.png 300w\" sizes=\"(max-width: 370px) 100vw, 370px\" \/><\/a><\/p>\n<p><span style=\"color: #000000;\"><b>\u9006\u8f6c\u5f55\u9176\u548cDNA\u805a\u5408\u9176<\/b><\/span><\/p>\n<p><a href=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2020\/10\/reverse-transcriptase-and-dna-polymerase-enzymes.png\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-11809 size-full\" src=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2020\/10\/reverse-transcriptase-and-dna-polymerase-enzymes.png\" alt=\"\" width=\"280\" height=\"143\" \/><\/a><\/p>\n<p><span style=\"color: #000000;\"><b>\u9996\u8981\u4efb\u52a1<\/b><\/span><\/p>\n<p><a href=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2020\/10\/primers.png\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-11810 size-full\" src=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2020\/10\/primers.png\" alt=\"\" width=\"177\" height=\"94\" \/><\/a><\/p>\n<p><span style=\"color: #000000;\"><b>\u6838\u82f7\u4e09\u78f7\u9178\u76d0<\/b><\/span><\/p>\n<p><a href=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2020\/10\/nucleoside-triphosphates.png\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone size-full wp-image-11811\" src=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2020\/10\/nucleoside-triphosphates.png\" alt=\"\" width=\"188\" height=\"70\" \/><\/a><\/p>\n<p><span style=\"color: #000000;\"><b>\u548c\u8367\u5149\u62a5\u544a\u5242\u67d3\u6599\u3002\u00a0<\/b><\/span><\/p>\n<p><span style=\"font-weight: 400;\">\u4e3a\u4e86\u4e86\u89e3\u4e00\u4e2a\u53ef\u80fd\u88abCOVID-19\u75c5\u6bd2\u6c61\u67d3\u6216\u4e0d\u88ab\u6c61\u67d3\u7684\u6837\u54c1\u7684\u7ed3\u679c\uff0c\u8367\u5149\u62a5\u544a\u67d3\u6599\u68c0\u6d4b\u4e0eRT-PCR\u68c0\u6d4b\u7ed3\u5408\u4f7f\u7528\u3002\u8be5\u68c0\u6d4b\u662f\u57fa\u4e8e\u6837\u54c1\u7684\u8367\u5149\u4fe1\u53f7\u7684\u5b58\u5728\u4e0e\u5426\u3002\u5728\u6ca1\u6709\u4fe1\u53f7\u7684\u60c5\u51b5\u4e0b\uff0c\u8be5\u8bd5\u9a8c\u53ef\u88ab\u65ad\u5b9a\u4e3a\u9634\u6027\u6216\u5047\u9634\u6027\u3002\u5728\u6709\u5f3a\u70c8\u4fe1\u53f7\u7684\u60c5\u51b5\u4e0b\uff0c\u4e0d\u4ec5\u53ef\u4ee5\u65ad\u5b9aV\u00edrus\u7684\u5b58\u5728\u4e3a\u9633\u6027\uff0c\u800c\u4e14\u53ef\u4ee5\u65ad\u5b9a\u4e3a\u665a\u671f\u75be\u75c5\u3002<\/span><\/p>\n<p><span style=\"color: #000000;\"><b>\u6df7\u5408\u6240\u6709\u6210\u5206\uff0c\u5e76\u5c06\u5176\u653e\u5165\u70ed\u5faa\u73af\u673a\u3002\u00a0<\/b><\/span><\/p>\n<p><span style=\"font-weight: 400;\">\u63a5\u4e0b\u6765\u7684\u4fe1\u606f\u56fe\u4e2d\u663e\u793a\u4e86\u6d4b\u8bd5\u7684\u7b2c\u4e00\u6b65\u3002RT\u9176\u5bf9\u6837\u54c1\u4e2d\u5b58\u5728\u7684\u75c5\u6bd2\u7684\u9057\u4f20\u7269\u8d28\u8fdb\u884c\u7b2c\u4e00\u6b21\u590d\u5236\u3002\u4e0b\u9762\u4f60\u770b\u5230RT-PCR\u65b9\u6cd5\u7684\u7b2c\u4e8c\u6b65\u3002\u5f15\u7269\u6307\u793a\u8d77\u59cb\u4f4d\u7f6e\uff0cDNA\u805a\u5408\u9176\u901a\u8fc7\u9176\u4fc3\u53cd\u5e94\u5f00\u59cb\u5ef6\u957fDNA\u94fe\u3002<\/span><\/p>\n<p><a href=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2020\/10\/mix-all-the-ingredients-and-put-it-in-the-thermocycle-machine.png\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-11812 size-full\" src=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2020\/10\/mix-all-the-ingredients-and-put-it-in-the-thermocycle-machine.png\" alt=\"\u51a0\u72b6\u75c5\u6bd2\u7684RT-PCR\u6d4b\u8bd5\" width=\"512\" height=\"150\" srcset=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2020\/10\/mix-all-the-ingredients-and-put-it-in-the-thermocycle-machine.png 512w, https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2020\/10\/mix-all-the-ingredients-and-put-it-in-the-thermocycle-machine-300x88.png 300w\" sizes=\"(max-width: 512px) 100vw, 512px\" \/><\/a><\/p>\n<p><a href=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2020\/10\/01-mix-all-the-ingredients-and-put-it-in-the-thermocycle-machine.png\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-11813 size-full\" src=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2020\/10\/01-mix-all-the-ingredients-and-put-it-in-the-thermocycle-machine.png\" alt=\"\" width=\"472\" height=\"384\" srcset=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2020\/10\/01-mix-all-the-ingredients-and-put-it-in-the-thermocycle-machine.png 472w, https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2020\/10\/01-mix-all-the-ingredients-and-put-it-in-the-thermocycle-machine-300x244.png 300w\" sizes=\"(max-width: 472px) 100vw, 472px\" \/><\/a><\/p>\n<p><a href=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2020\/10\/02-mix-all-the-ingredients-and-put-it-in-the-thermocycle-machine.png\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-11814 size-full\" src=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2020\/10\/02-mix-all-the-ingredients-and-put-it-in-the-thermocycle-machine.png\" alt=\"\" width=\"466\" height=\"364\" srcset=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2020\/10\/02-mix-all-the-ingredients-and-put-it-in-the-thermocycle-machine.png 466w, https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2020\/10\/02-mix-all-the-ingredients-and-put-it-in-the-thermocycle-machine-300x234.png 300w\" sizes=\"(max-width: 466px) 100vw, 466px\" \/><\/a><\/p>\n<p><span style=\"font-weight: 400;\">\u6700\u540e\uff0c\u8fd9\u5f20\u56fe\u7247\u662fSARS-CoV-2\u75c5\u6bd2\u7684\u7535\u5b50\u663e\u5fae\u56fe\u50cf\uff0c\u53ef\u4ee5\u5b8c\u7f8e\u5730\u770b\u5230\u75c5\u6bd2\u7684\u7ed3\u6784\u3002\u8fd9\u4e0d\u662f\u5f88\u7f8e\u5417\uff1f\u00a0\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400;\">\u8fd9\u4e2a\u6d4b\u8bd5\u5f88\u590d\u6742\uff0c\u5e76\u4e0d\u50cf\u770b\u8d77\u6765\u90a3\u4e48\u7b80\u5355\uff0c\u4f46\u6211\u6562\u6253\u8d4c\uff0c\u6709\u4e86\u4fe1\u606f\u56fe\u8868\uff0c\u5c31\u66f4\u5bb9\u6613\u7406\u89e3\u5b83\u662f\u5982\u4f55\u8fd0\u4f5c\u7684\u5927\u753b\u9762\u4e86\uff0c\u5bf9\u5417\uff1f<\/span><\/p>\n<p><span style=\"font-weight: 400;\">\u4f60\u53ef\u4ee5\u7528\u4efb\u4f55\u4f60\u60f3\u8981\u7684\u79d1\u5b66\u4fe1\u606f\u6765\u505a\u8fd9\u4ef6\u4e8b\uff0c\u4f7f\u7528\u00a0<\/span><a href=\"https:\/\/mindthegraph.com\/\"> <span style=\"font-weight: 400;\">Mind the Graph<\/span><\/a><span style=\"font-weight: 400;\">.\u8fd9\u4e2a\u5e73\u53f0\u662f\u4e3a\u4e86\u8ba9\u4f60\u6709\u81ea\u4e3b\u6743\uff0c\u66f4\u597d\u5730\u4ea4\u6d41\u79d1\u5b66\u4fe1\u606f\u3002\u4e86\u89e3\u66f4\u591a\u5173\u4e8e\u5982\u4f55\u4f7f\u7528\u8be5\u5e73\u53f0\uff0c\u5982\u679c\u4f60\u9700\u8981\u4efb\u4f55\u5e2e\u52a9\uff0c\u8bf7\u8054\u7cfb\u6211\u4eec<\/span><b>!<\/b><\/p>\n<p><b>\u53c2\u8003\u8d44\u6599<\/b><\/p>\n<p><span style=\"font-weight: 400;\">AI, T. \u7b49. \u4e2d\u56fd2019\u5e74\u51a0\u72b6\u75c5\u6bd2\u75c5\uff08COVID-19\uff09\u80f8\u90e8CT\u548cRT-PCR\u68c0\u6d4b\u7684\u76f8\u5173\u6027\u30021014\u4f8b\u7684\u62a5\u544a\u3002Radiology, v. 296, n. 2, p. E32-E40, 26 fev. 2020.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">GIBELLINI, D. et al.\u7528SYBR\u7eff\u8272\u5b9e\u65f6RT-PCR\u6280\u672f\u5b9a\u91cf\u68c0\u6d4bHIV-1\u8840\u6e05\u9633\u6027\u60a3\u8005\u7684\u4eba\u7c7b\u514d\u75ab\u7f3a\u9677\u75c5\u6bd21\u578b\uff08HIV-1\uff09\u75c5\u6bd2\u91cf\u3002\u75c5\u6bd2\u5b66\u65b9\u6cd5\u6742\u5fd7\u300b\uff0c\u7b2c115\u671f\uff0c\u7b2c2\u671f\uff0c\u7b2c183-189\u9875\uff0c2004\u5e747\u67081\u65e5\u3002<\/span><\/p>\n<p><span style=\"font-weight: 400;\">JAMES, D. \u901a\u8fc7RT-PCR\u548c\u591a\u91cdPCR\u68c0\u6d4b\u82f9\u679c\u830e\u90e8\u5f00\u69fd\u75c5\u6bd2\u7684\u7b80\u5355\u800c\u53ef\u9760\u7684\u65b9\u6848\u3002\u75c5\u6bd2\u5b66\u65b9\u6cd5\u6742\u5fd7\u300b\uff0c\u7b2c83\u671f\uff0c\u7b2c1\u9875\uff0c\u7b2c1-9\u9875\uff0c1999\u5e7412\u67081\u65e5\u3002<\/span><\/p>\n<p><span style=\"font-weight: 400;\">LI, Y. et al. \u5bf9\u4e34\u5e8a\u8bca\u65ad\u4e3aCOVID-19\u7684\u4f4f\u9662\u60a3\u8005\u8fdb\u884cSARS-CoV-2\u7684RT-PCR\u68c0\u6d4b\u7684\u7a33\u5b9a\u6027\u95ee\u9898\u3002Journal of Medical Virology, v. 92, n. 7, p. 903-908, 2020.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">LIAO, C.-S. et al. Miniature RT-PCR system for diagnosis of RNA-based viruses.Nucleic Acids Research, v. 33, n. 18, p. e156-e156, 1 out.2005.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">MOHAN, A. et al. Prevalence of viral infection detected by PCR and RT-PCR in patients with acute exacerbation of COPD: A systematic review.Respirology, v. 15, n. 3, p. 536-542, 2010.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">NOLAN, T.; HANDS, R. E.; BUSTIN, S. A. \u4f7f\u7528\u5b9e\u65f6RT-PCR\u5bf9mRNA\u8fdb\u884c\u91cf\u5316\u3002Nature Protocols, v. 1, n. 3, p. 1559-1582, ago.2006.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">\u65b0\u578b\u51a0\u72b6\u75c5\u6bd2SARS-CoV-2 | \u8fd9\u4e2a\u900f\u5c04\u7535\u955c\u662f\u5728Flickr\u4e0a\u770b\u5230\u7684\u3002\u53ef\u5728\u6b64\u4e0b\u8f7d\u3002.\u8bbf\u95ee\u65f6\u95f4\uff1a2020\u5e747\u670823\u65e5\u3002 <\/span><\/p>","protected":false},"excerpt":{"rendered":"","protected":false},"author":4,"featured_media":11847,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[959,28],"tags":[388,799,554,798,250],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v19.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>The RT-PCR Test for Coronavirus - Mind the Graph Blog<\/title>\n<meta name=\"description\" content=\"Read this blog to know everything related to the popular COVID-19 test, RT-PCR or only PCR. 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He has a Ph.D. and solid scientific background in Psychopharmacology and experience as a Guest Researcher at the Max Planck Institute of Psychiatry (Germany) and Researcher in D'Or Institute for Research and Education (IDOR, Brazil). Fabricio holds over 2500 citations in Google Scholar. He has 10 years of experience in small innovative businesses, with relevant experience in product design and innovation management. Connect with him on LinkedIn - Fabricio Pamplona.","sameAs":["http:\/\/mindthegraph.com","https:\/\/www.linkedin.com\/in\/fabriciopamplona"],"url":"https:\/\/mindthegraph.com\/blog\/zh\/author\/fabricio\/"}]}},"_links":{"self":[{"href":"https:\/\/mindthegraph.com\/blog\/zh\/wp-json\/wp\/v2\/posts\/11803"}],"collection":[{"href":"https:\/\/mindthegraph.com\/blog\/zh\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/mindthegraph.com\/blog\/zh\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/mindthegraph.com\/blog\/zh\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/mindthegraph.com\/blog\/zh\/wp-json\/wp\/v2\/comments?post=11803"}],"version-history":[{"count":3,"href":"https:\/\/mindthegraph.com\/blog\/zh\/wp-json\/wp\/v2\/posts\/11803\/revisions"}],"predecessor-version":[{"id":11850,"href":"https:\/\/mindthegraph.com\/blog\/zh\/wp-json\/wp\/v2\/posts\/11803\/revisions\/11850"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/mindthegraph.com\/blog\/zh\/wp-json\/wp\/v2\/media\/11847"}],"wp:attachment":[{"href":"https:\/\/mindthegraph.com\/blog\/zh\/wp-json\/wp\/v2\/media?parent=11803"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/mindthegraph.com\/blog\/zh\/wp-json\/wp\/v2\/categories?post=11803"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/mindthegraph.com\/blog\/zh\/wp-json\/wp\/v2\/tags?post=11803"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}