{"id":16636,"date":"2022-09-10T18:27:00","date_gmt":"2022-09-10T21:27:00","guid":{"rendered":"https:\/\/mindthegraph.com\/blog\/?p=16636"},"modified":"2023-03-17T14:47:45","modified_gmt":"2023-03-17T17:47:45","slug":"success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids","status":"publish","type":"post","link":"https:\/\/mindthegraph.com\/blog\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/","title":{"rendered":"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review"},"content":{"rendered":"\n

The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells is much easier to understand using Mind the Graph\u2019s illustrations. <\/p>\n\n\n\n

The scheme created by the author shows that the surgical biopsies were enzymatically digested to obtain a suspension of single cells. The na\u00efve unsorted cells were cultured in FGF2 containing CSC-medium or with the FGFR-inhibitor SU-5402.<\/p>\n\n\n\n

The role of fibroblast growth factor 2 (FGF2) in the self-renewal of colorectal cancer stem cells has been investigated<\/strong><\/h2>\n\n\n\n

In \u2018FGF Signalling in the Self-Renewal of Colon Cancer Organoids\u2019, J\u00f6rg Otte and colleagues (2019) reported that when na\u00efve single cells were embedded in Matrigel and cultured in the CSC-medium, a more complex self-organization was observed in all patients analysed. <\/p>\n\n\n\n

Genes directly associated with EMT together with a mesenchymal and self-renewing phenotype (TGFB3, ID1, ID2 and ID4) were found to be overexpressed in organoids. <\/p>\n\n\n\n

While FGFR-inhibition led to an enhanced MAPK signalling, they observed induced differentiation, a loss of most stem cell markers and an epithelial phenotype. <\/p>\n\n\n\n

Cancer stem cells are a subpopulation of malignant cells able to self-renew and to serve as an ongoing source for differentiated tumour cells. In some protocols of CSC culture, Fibroblast growth factor 2 is an important ingredient for maintaining stemness. <\/p>\n\n\n\n

In CSC-medium cultured organoids, on the other hand, they detected induction of TGF-\u03b2 and of all four members of the inhibitor-of-differentiation gene family.<\/p>\n\n\n\n

9 consecutive unselected CRC patients were involved in the research.<\/strong><\/p>\n\n\n\n

Check the original article here<\/a>.<\/p>\n\n\n\n

Graphical Abstract powered by Mind the Graph<\/strong><\/h2>\n\n\n\n
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Made with Mind the Graph<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

Some of their conclusions potentially strengthen what was previously known about this field: \u201cThe pluripotency-associated genes NANOG, LIN28 and SALL1 were exclusively expressed by hESCs. The GO-terms \u201cnegative regulation of BMP signaling pathway\u201d and \u201cSMAD protein signal transduction\u201d were detected in our hESC culture,\u201d Otte suggested.<\/p>\n\n\n\n

The authors admit that \u201cGO-term analysis of this gene-set yielded only a few significant results due to the low number of commonly regulated genes. Most of these GO-terms for the CSC-medium condition were associated with active transcription, cell proliferation or increased glucose uptake. <\/p>\n\n\n\n

After FGFR-inhibition, we found cell-cycle regulators as well as many genes annotated with \u201cepithelial cell differentiation\u201d, \u201cdigestion\u201d or \u201cbile acid and bile salt transport\u201d.\u201d<\/p>\n\n\n\n

Papers Impact and Performance<\/strong><\/h2>\n\n\n\n

We can analyze with Altmetric<\/a> data that this paper’s online attention score is higher than 68%<\/strong><\/strong> of all other articles of a similar age in Scientific Reports.<\/strong><\/p>\n\n\n\n

<\/div>\n\n\n
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Source Altmetrics<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

Improve your article’s accessibility with the power of infographics as well.<\/p>\n\n\n\n

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Try Mind the Graph<\/strong><\/a><\/div>\n<\/div>\n\n\n\n
<\/div>\n","protected":false},"excerpt":{"rendered":"

The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.<\/p>\n","protected":false},"author":29,"featured_media":20014,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[195,958,957,1],"tags":[96,554,775,963],"yoast_head":"\nFGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog<\/title>\n<meta name=\"description\" content=\"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mindthegraph.com\/blog\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - 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