{"id":8728,"date":"2019-04-23T16:35:01","date_gmt":"2019-04-23T19:35:01","guid":{"rendered":"https:\/\/blog.mindthegraph.com\/?p=8728"},"modified":"2022-10-18T08:23:59","modified_gmt":"2022-10-18T11:23:59","slug":"visual-abstract-example","status":"publish","type":"post","link":"https:\/\/mindthegraph.com\/blog\/tr\/gorsel-soyut-ornek\/","title":{"rendered":"Yelena Sterlin'in \"Optimize Edilmi\u015f K\u00fc\u00e7\u00fck Molek\u00fcll\u00fc Pull-Down'lar MLBP1'i Bir Asil-Lipid Ba\u011flay\u0131c\u0131 Protein Olarak Tan\u0131ml\u0131yor\" ba\u015fl\u0131kl\u0131 ara\u015ft\u0131rma makalesini a\u00e7\u0131klamak i\u00e7in kullan\u0131c\u0131 taraf\u0131ndan olu\u015fturulan g\u00f6rsel \u00f6zet"},"content":{"rendered":"<p style=\"text-align: justify;\">G\u00f6rsel bir soyut olu\u015fturmak kolay de\u011fildir, ancak kal\u0131c\u0131d\u0131r. Bu nedenle, kullan\u0131c\u0131lar\u0131m\u0131z\u0131n harika \u00e7al\u0131\u015fmalar\u0131n\u0131 ilham verici bir g\u00f6rsel soyut \u00f6rne\u011fi olarak g\u00f6stermeyi her zaman seviyoruz. Bilim insanlar\u0131n\u0131 daha g\u00f6rsel ve ula\u015f\u0131labilir bir bilimi m\u00fcmk\u00fcn k\u0131lmalar\u0131 i\u00e7in g\u00fc\u00e7lendirmek istiyoruz.<\/p>\n<p style=\"text-align: justify;\">Bu yaz\u0131da, size a\u015fa\u011f\u0131daki orijinal ara\u015ft\u0131rman\u0131n bir k\u0131sm\u0131n\u0131 g\u00f6sterece\u011fim&nbsp;<a href=\"https:\/\/www.researchgate.net\/profile\/Yelena_Sterlin2\" target=\"_blank\" rel=\"noopener\">Yelena Sterlin<\/a> ve meslekta\u015flar\u0131. Kud\u00fcs \u0130brani \u00dcniversitesi Tar\u0131m, G\u0131da ve \u00c7evre Fak\u00fcltesi'nde doktora \u00f6\u011frencisidir.<\/p>\n<p style=\"text-align: justify;\">Yeni makalesi&nbsp;<a href=\"https:\/\/www.researchgate.net\/publication\/330976296_Optimized_Small-Molecule_Pull-Downs_Define_MLBP1_as_an_Acyl-Lipid-Binding_Protein\" target=\"_blank\" rel=\"noopener\">Optimize Edilmi\u015f K\u00fc\u00e7\u00fck Molek\u00fcll\u00fc Pull-Down'lar MLBP1'i Asil-Lipid Ba\u011flay\u0131c\u0131 Protein Olarak Tan\u0131ml\u0131yor<\/a><strong>&nbsp;<\/strong>Absisik asit (ABA) resept\u00f6rlerine odaklanarak bitkilerdeki protein-metabolit etkile\u015fimlerine \u0131\u015f\u0131k tutuyor. Kendisi ayn\u0131 zamanda bir Mind the Graph abonesi, bu y\u00fczden bir infografik olu\u015fturdu <a href=\"https:\/\/mindthegraph.com\">grafi\u011fe dikkat edin<\/a> metodolojilerini a\u00e7\u0131klamak i\u00e7in.<\/p>\n<h2 style=\"text-align: center;\"><strong>START alan\u0131 ile ili\u015fkili proteinlerin \u00f6nemi<\/strong><\/h2>\n<p>&nbsp;<\/p>\n<p style=\"text-align: justify;\">G\u00f6re <a href=\"https:\/\/www.nature.com\/articles\/s41598-018-22538-9\" target=\"_blank\" rel=\"noopener\">Do\u011fa Dergisi<\/a>absisik asit (ABA), abiyotik stres tepkisinde ve adaptasyonunda rol oynayan ana fitohormondur. Ayr\u0131ca, aday bir tar\u0131msal kimyasald\u0131r.  ABA ilk olarak 1960'larda ke\u015ffedilmi\u015ftir. Bundan sonra, dormansi, \u00e7imlenme, tohum geli\u015fimi ve biyotik ve abiyotik strese verilen tepkiler gibi \u00e7e\u015fitli s\u00fcre\u00e7lerin \u00f6nemli bir d\u00fczenleyicisi oldu\u011fu bulunmu\u015ftur.<\/p>\n<p style=\"text-align: justify;\">ABA sinyal yolunu i\u00e7eren en erken olaylar, ABA'n\u0131n PYR\/PYL\/RCARs resept\u00f6rlerine ba\u011flanmas\u0131n\u0131 i\u00e7erir. Resept\u00f6rler START-domain s\u00fcper ailesine aittir.<\/p>\n<p style=\"text-align: justify;\">START protein alanlar\u0131 bakteri, protist, maya, bitki ve hayvan genomlar\u0131nda bulunan lipit ba\u011flay\u0131c\u0131 bir aland\u0131r. Ancak bitkilerde daha yayg\u0131nd\u0131r. Buna g\u00f6re<a href=\"https:\/\/genomebiology.biomedcentral.com\/articles\/10.1186\/gb-2004-5-6-r41\" target=\"_blank\" rel=\"noopener\"> Schrick<\/a>START alanlar\u0131n\u0131n hayvanlar ve bitkiler gibi evrimsel olarak uzak t\u00fcrlerde bulunmas\u0131, proteinlerin lipidler\/steroller ile etkile\u015fimi i\u00e7in korunmu\u015f bir mekanizma oldu\u011funu g\u00f6stermektedir.<\/p>\n<p style=\"text-align: justify;\">Dergide yay\u0131nlanan makale <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/30735592\" target=\"_blank\" rel=\"noopener\">The Plant Journal<\/a> PYR\/PYL\/RCARs ABA resept\u00f6rlerine odaklanmaktad\u0131r.  Ara\u015ft\u0131rmac\u0131lar, bitkilerde START domaini ve START domaini ile ili\u015fkili protein ligandlar\u0131n\u0131n tan\u0131mlanmas\u0131n\u0131 sa\u011flayacak bir y\u00f6ntemi optimize etmeye karar verdiler, \u00e7\u00fcnk\u00fc<em>&nbsp;\"START alan\u0131 protein ligandlar\u0131, hem hayvanlarda hem de bitkilerde proteinlerinin d\u00fczg\u00fcn \u00e7al\u0131\u015fmas\u0131 i\u00e7in vazge\u00e7ilmezdir ve bitki START alan\u0131 proteinlerinin temel fizyolojik s\u00fcre\u00e7lere dahil oldu\u011fu g\u00f6sterilmi\u015ftir\".<\/em><\/p>\n<h2 style=\"text-align: center;\"><strong>Metodolojinin g\u00f6rsel bir \u00f6zeti<\/strong><\/h2>\n<p>&nbsp;<\/p>\n<h4 style=\"text-align: center;\"><strong>Bilim insanlar\u0131, metodolojilerini a\u00e7\u0131klamak i\u00e7in zihin grafi\u011fi ill\u00fcstrasyonlar\u0131yla bir infografik olu\u015fturdular. G\u00f6z al\u0131c\u0131 ve anla\u015f\u0131lmas\u0131 daha kolay.&nbsp;<\/strong><\/h4>\n<p>&nbsp;<\/p>\n<p style=\"text-align: justify;\">A\u015fa\u011f\u0131daki infografik deneysel i\u015f ak\u0131\u015f\u0131n\u0131 g\u00f6stermektedir. G\u00f6rd\u00fc\u011f\u00fcn\u00fcz gibi, d\u00f6rt START proteinini ifade ettiler ve safla\u015ft\u0131rd\u0131lar. Do\u011fal \u00e7\u00f6z\u00fcn\u00fcr fraksiyon i\u00e7eren s\u00fcpernatant\u0131, g\u00fcvenilir ve y\u00fcksek \u00e7\u00f6z\u00fcn\u00fcrl\u00fck i\u00e7in bir afinite re\u00e7inesi olan streptavidin sepharose kullanarak filtrelediler ve ink\u00fcbe ettiler.<wbr>-biyomolek\u00fcllerin \u00e7\u00f6z\u00fcn\u00fcrl\u00fckl\u00fc safla\u015ft\u0131r\u0131lmas\u0131. Ard\u0131ndan, western blot kullanarak alikotlar\u0131 analiz ettiler. Ligand\u0131 tan\u0131mlamak i\u00e7in, varsay\u0131lan ligandlar\u0131 el\u00fce ettiler ve bir LC -MS (s\u0131v\u0131 kromatografisi ve k\u00fctle spektrometresi) sistemi analizine tabi tuttular. \u0130lgilenilen varsay\u0131lan ligandlar\u0131n molek\u00fcler yap\u0131lar\u0131 tandem k\u00fctle spektrometresi ile belirlenmi\u015ftir.<\/p>\n<p>&nbsp;<\/p>\n<p style=\"text-align: justify;\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-8735 size-full\" src=\"https:\/\/blog.mindthegraph.com\/wp-content\/uploads\/2019\/04\/yelena.png\" alt=\"yelena sterlin&#039;den g\u00f6rsel soyut \" width=\"610\" height=\"650\" srcset=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2019\/04\/yelena.png 610w, https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2019\/04\/yelena-282x300.png 282w\" sizes=\"(max-width: 610px) 100vw, 610px\" \/><\/p>\n<p style=\"text-align: justify;\">Yazarlar, bitkilerdeki protein ligandlar\u0131n\u0131n ilk ara\u015ft\u0131rmas\u0131 i\u00e7in iyi bir teknik sunmu\u015flard\u0131r. START ligandlar\u0131n\u0131 ke\u015ffetmek i\u00e7in yakla\u015f\u0131m\u0131 ayarlad\u0131lar. Bununla birlikte, analitik ko\u015fullar\u0131n ayarlanmas\u0131yla y\u00f6ntemi farkl\u0131 kimyasal yap\u0131daki ligandlara uyarlayabilirsiniz.<\/p>\n<p style=\"text-align: justify;\">Ara\u015ft\u0131rman\u0131z\u0131 bilimsel ill\u00fcstrasyonlar ve g\u00f6rsel \u00f6zetler kullanarak g\u00f6stermek istiyorsan\u0131z, Mind the Graph'yi de kullanabilirsiniz. Peki, bilim ileti\u015fiminizi geli\u015ftirmeye haz\u0131r m\u0131s\u0131n\u0131z?<\/p>\n<p style=\"text-align: justify;\"><a href=\"https:\/\/mindthegraph.com\" target=\"_blank\" rel=\"noopener\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-6577 size-full\" src=\"https:\/\/blog.mindthegraph.com\/wp-content\/uploads\/2017\/01\/start_get-mind-the-graph-medical-illustrations.png\" alt=\"preview_250\" width=\"310\" height=\"100\" srcset=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2017\/01\/start_get-mind-the-graph-medical-illustrations.png 310w, https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2017\/01\/start_get-mind-the-graph-medical-illustrations-300x97.png 300w\" sizes=\"(max-width: 310px) 100vw, 310px\" \/><\/a><\/p>","protected":false},"excerpt":{"rendered":"<p>G\u00f6rsel bir soyut olu\u015fturmak kolay de\u011fildir, ancak kal\u0131c\u0131d\u0131r. Bu nedenle, kullan\u0131c\u0131lar\u0131m\u0131z\u0131n harika \u00e7al\u0131\u015fmalar\u0131n\u0131 ilham verici bir g\u00f6rsel soyut \u00f6rne\u011fi olarak g\u00f6stermeyi her zaman seviyoruz. Bilim insanlar\u0131n\u0131 daha g\u00f6rsel ve eri\u015filebilir bir bilimi m\u00fcmk\u00fcn k\u0131lmalar\u0131 i\u00e7in g\u00fc\u00e7lendirmek istiyoruz. Bu yaz\u0131da size biraz [...]<\/p>","protected":false},"author":11,"featured_media":8741,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"image","meta":[],"categories":[193,40],"tags":[291,96,292,245,294,295,554,775,293,382],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v19.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Visual abstract created by Mind the Graph user. 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