{"id":16636,"date":"2022-09-10T18:27:00","date_gmt":"2022-09-10T21:27:00","guid":{"rendered":"https:\/\/mindthegraph.com\/blog\/?p=16636"},"modified":"2023-03-17T14:47:45","modified_gmt":"2023-03-17T17:47:45","slug":"success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids","status":"publish","type":"post","link":"https:\/\/mindthegraph.com\/blog\/sv_se\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/","title":{"rendered":"FGF-signalering vid sj\u00e4lvf\u00f6rnyelse av organoider f\u00f6r tjocktarmscancer Paper Review"},"content":{"rendered":"

Den inaktiverade sf\u00e4roid- och organoidbildningen genom FGFR-h\u00e4mning i patientderiverade CRC-celler \u00e4r mycket l\u00e4ttare att f\u00f6rst\u00e5 med Mind the Graph:s illustrationer. <\/p>\n\n\n\n

Det schema som f\u00f6rfattaren skapat visar att de kirurgiska biopsierna sm\u00e4ltes enzymatiskt f\u00f6r att f\u00e5 en suspension av enskilda celler. De naiva osorterade cellerna odlades i FGF2-inneh\u00e5llande CSC-medium eller med FGFR-inhibitorn SU-5402.<\/p>\n\n\n\n

Fibroblasttillv\u00e4xtfaktor 2:s (FGF2) roll i sj\u00e4lvf\u00f6rnyelsen av stamceller fr\u00e5n kolorektalcancer har unders\u00f6kts<\/strong><\/h2>\n\n\n\n

I artikeln \"FGF Signalling in the Self-Renewal of Colon Cancer Organoids\" rapporterade J\u00f6rg Otte och medarbetare (2019) att n\u00e4r naiva enskilda celler b\u00e4ddades in i Matrigel och odlades i CSC-medium observerades en mer komplex sj\u00e4lvorganisering hos alla patienter som analyserades. <\/p>\n\n\n\n

Gener som \u00e4r direkt associerade med EMT tillsammans med en mesenkymal och sj\u00e4lvf\u00f6rnyande fenotyp (TGFB3, ID1, ID2 och ID4) visade sig vara \u00f6veruttryckta i organoider. <\/p>\n\n\n\n

Medan FGFR-h\u00e4mning ledde till en \u00f6kad MAPK-signalering, observerade de inducerad differentiering, en f\u00f6rlust av de flesta stamcellsmark\u00f6rer och en epitelial fenotyp. <\/p>\n\n\n\n

Cancerstamceller \u00e4r en subpopulation av maligna celler som kan f\u00f6rnya sig sj\u00e4lva och fungera som en kontinuerlig k\u00e4lla f\u00f6r differentierade tum\u00f6rceller. I vissa protokoll f\u00f6r odling av CSC \u00e4r fibroblasttillv\u00e4xtfaktor 2 en viktig ingrediens f\u00f6r att bibeh\u00e5lla stamcellen. <\/p>\n\n\n\n

I organoider som odlats med CSC-medium fann de d\u00e4remot induktion av TGF-\u03b2 och av alla fyra medlemmarna i genfamiljen inhibitor-of-differentiation.<\/p>\n\n\n\n

9 konsekutiva, oselekterade CRC-patienter deltog i unders\u00f6kningen.<\/strong><\/p>\n\n\n\n

Kontrollera originalartikel h\u00e4r<\/a>.<\/p>\n\n\n\n

Grafisk sammanfattning baserad p\u00e5 Mind the Graph<\/strong><\/h2>\n\n\n\n
<\/div>\n\n\n
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Tillverkad med Mind the Graph<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

Vissa av deras slutsatser st\u00e4rker potentiellt det som tidigare var k\u00e4nt inom detta omr\u00e5de: \"De pluripotensassocierade generna NANOG, LIN28 och SALL1 uttrycktes uteslutande av hESC. GO-termerna \"negativ reglering av BMP-signalv\u00e4gen\" och \"SMAD-proteinsignaltransduktion\" uppt\u00e4cktes i v\u00e5r hESC-kultur\", f\u00f6reslog Otte.<\/p>\n\n\n\n

F\u00f6rfattarna medger att \"GO-termanalys av denna genupps\u00e4ttning gav endast ett f\u00e5tal signifikanta resultat p\u00e5 grund av det l\u00e5ga antalet vanligt reglerade gener. De flesta av dessa GO-termer f\u00f6r CSC-medium-tillst\u00e5ndet var associerade med aktiv transkription, cellproliferation eller \u00f6kat glukosupptag. <\/p>\n\n\n\n

Efter FGFR-h\u00e4mning fann vi cellcykelregulatorer samt m\u00e5nga gener som kommenterats med \"differentiering av epitelceller\", \"matsm\u00e4ltning\" eller \"transport av gallsyra och gallsalter\".\"<\/p>\n\n\n\n

Pappers p\u00e5verkan och prestanda<\/strong><\/h2>\n\n\n\n

Vi kan analysera med Altmetrisk<\/a> uppgifter om att denna tidnings uppm\u00e4rksamhetspo\u00e4ng online \u00e4r h\u00f6gre \u00e4n 68%<\/strong><\/strong> av alla andra artiklar av liknande \u00e5lder i Scientific Reports.<\/strong><\/p>\n\n\n\n

<\/div>\n\n\n
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K\u00e4lla Altmetri<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

F\u00f6rb\u00e4ttra din artikels tillg\u00e4nglighet med hj\u00e4lp av infografik.<\/p>\n\n\n\n

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F\u00f6rs\u00f6k Mind the Graph<\/strong><\/a><\/div>\n<\/div>\n\n\n\n
<\/div>","protected":false},"excerpt":{"rendered":"

Den inaktiverade sf\u00e4roid- och organoidbildningen genom FGFR-h\u00e4mning i patientderiverade CRC-celler \u00e4r l\u00e4ttare att f\u00f6rst\u00e5 med v\u00e5ra illustrationer.<\/p>","protected":false},"author":29,"featured_media":20014,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[195,958,957,1],"tags":[96,554,775,963],"yoast_head":"\nFGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog<\/title>\n<meta name=\"description\" content=\"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mindthegraph.com\/blog\/sv_se\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/\" \/>\n<meta property=\"og:locale\" content=\"sv_SE\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - 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