{"id":8728,"date":"2019-04-23T16:35:01","date_gmt":"2019-04-23T19:35:01","guid":{"rendered":"https:\/\/blog.mindthegraph.com\/?p=8728"},"modified":"2022-10-18T08:23:59","modified_gmt":"2022-10-18T11:23:59","slug":"visual-abstract-example","status":"publish","type":"post","link":"https:\/\/mindthegraph.com\/blog\/sl\/visual-abstract-example\/","title":{"rendered":"Vizualni povzetek, ki ga je ustvaril uporabnik za razlago raziskovalnega \u010dlanka \"Optimized Small-Molecule Pull-Downs Define MLBP1 as an Acyl-Lipid-Binding Protein\" avtorice Yelene Sterlin"},"content":{"rendered":"

Vizualne abstrakcije ni lahko ustvariti, vendar je tu, da ostane. Zato vedno radi poka\u017eemo neverjetna dela na\u0161ih uporabnikov kot navdihujo\u010d primer vizualne abstrakcije. Znanstvenike \u017eelimo opolnomo\u010diti, da bi omogo\u010dili bolj vizualno in dosegljivo znanost.<\/p>\n

V prispevku vam bom predstavil del\u010dek izvirne raziskave, ki jo je Yelena Sterlin<\/a> in sodelavci. Je doktorska \u0161tudentka na Fakulteti za kmetijstvo, prehrano in okolje na Hebrejski univerzi v Jeruzalemu.<\/p>\n

Njen novi \u010dlanek Optimizirani izvle\u010dki majhnih molekul opredeljujejo MLBP1 kot beljakovino, ki ve\u017ee acil-lipide<\/a> <\/strong>osvetljuje interakcije med proteini in metaboliti v rastlinah, s poudarkom na receptorjih za abscisi\u010dno kislino (ABA). Je tudi naro\u010dnica programa mind the graph, zato je ustvarila infografiko v upo\u0161tevajte graf<\/a> pojasniti svojo metodologijo.<\/p>\n

Pomen proteinov, povezanih z domeno START<\/strong><\/h2>\n

 <\/p>\n

Po podatkih Revija Nature<\/a>abscisi\u010dna kislina (ABA) je glavni fitohormon, ki sodeluje pri odzivanju na abiotski stres in prilagajanju nanj. Prav tako je kandidat za agrokemikalije. ABA je bila prvi\u010d odkrita v \u0161estdesetih letih prej\u0161njega stoletja. Nato je bilo ugotovljeno, da je klju\u010dni regulator razli\u010dnih procesov, kot so mirovanje, kalitev, razvoj semen ter odzivi na biotski in abiotski stres.<\/p>\n

Najzgodnej\u0161i dogodki, ki vklju\u010dujejo signalno pot ABA, vklju\u010dujejo vezavo ABA na receptorje PYR\/PYL\/RCARs. Receptorji spadajo v naddru\u017eino z domeno START.<\/p>\n

Proteinske domene START so domene, ki ve\u017eejo lipide in so prisotne v genomih bakterij, protistov, kvasovk, rastlin in \u017eivali. Vendar so pogostej\u0161e pri rastlinah. Po podatkih Schrick<\/a>, prisotnost domen START pri evolucijsko oddaljenih vrstah, kot so \u017eivali in rastline, ka\u017ee na ohranjen mehanizem interakcije proteinov z lipidi\/steroli.<\/p>\n

Dokument, objavljen v \u010casopis za rastline<\/a> se osredoto\u010da na receptorje ABA PYR\/PYL\/RCAR. Raziskovalci so se odlo\u010dili optimizirati metodo, ki bi omogo\u010dila identifikacijo ligandov domene START in z domeno START povezanih proteinov v rastlinah, ker \"ligandi proteinov z domeno START so nepogre\u0161ljivi za pravilno delovanje njihovih proteinov tako pri \u017eivalih kot pri rastlinah, za rastlinske proteine z domeno START pa je bilo dokazano, da so vpleteni v bistvene fiziolo\u0161ke procese\".<\/em><\/p>\n

Vizualni povzetek metodologije<\/strong><\/h2>\n

 <\/p>\n

Da bi pojasnili svojo metodologijo, so znanstveniki pripravili infografiko z ilustracijami grafov. Ta je privla\u010dna za o\u010di in la\u017eje razumljiva. <\/strong><\/h4>\n

 <\/p>\n

Spodnja infografika prikazuje eksperimentalni potek dela. Kot lahko vidite, so izrazili in o\u010distili \u0161tiri beljakovine START. Supernatant, ki je vseboval nativno topno frakcijo, so filtrirali in inkubirali s streptavidinsko sefarozo, afinitetno smolo za zanesljivo in visoko-\u010di\u0161\u010denje biomolekul z visoko lo\u010dljivostjo. Nato so alikvote analizirali z metodo western blot. Za identifikacijo liganda so eluirali domnevne ligande in jih analizirali s sistemom LC-MS (teko\u010dinska kromatografija in masna spektrometrija). S tandemsko masno spektrometrijo so dolo\u010dili molekulsko strukturo domnevnih ligandov, ki so jih zanimali.<\/p>\n

 <\/p>\n

\"vizualna<\/p>\n

Avtorji so predstavili dobro tehniko za za\u010detno sondiranje beljakovinskih ligandov v rastlinah. Prilagodili so pristop za odkrivanje ligandov START. S prilagoditvijo analiti\u010dnih pogojev pa lahko metodo uporabimo za ligande razli\u010dne kemijske narave.<\/p>\n

\u010ce \u017eelite svoje raziskave prikazati z znanstvenimi ilustracijami in vizualnimi povzetki, lahko uporabite tudi Mind the Graph. Ste pripravljeni izbolj\u0161ati svoje sporazumevanje v znanosti?<\/p>\n

\"predogled_250\"<\/a><\/p>","protected":false},"excerpt":{"rendered":"

Vizualne abstrakcije ni lahko ustvariti, vendar je tu, da ostane. Zato vedno radi poka\u017eemo neverjetna dela na\u0161ih uporabnikov kot navdihujo\u010d primer vizualne abstrakcije. Znanstvenike \u017eelimo opolnomo\u010diti, da bi omogo\u010dili bolj vizualno in dosegljivo znanost. V tem prispevku vam bom prikazal del\u010dek [...]<\/p>","protected":false},"author":11,"featured_media":8741,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"image","meta":[],"categories":[193,40],"tags":[291,96,292,245,294,295,554,775,293,382],"yoast_head":"\nVisual abstract created by Mind the Graph user. Attractive scientific figure!<\/title>\n<meta name=\"description\" content=\"See the visual abstract created by the MindtheGraph subscriber Yelena Sterling to explain her research in an attractive and instructive way. 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