{"id":16636,"date":"2022-09-10T18:27:00","date_gmt":"2022-09-10T21:27:00","guid":{"rendered":"https:\/\/mindthegraph.com\/blog\/?p=16636"},"modified":"2023-03-17T14:47:45","modified_gmt":"2023-03-17T17:47:45","slug":"success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids","status":"publish","type":"post","link":"https:\/\/mindthegraph.com\/blog\/sl\/uspeh-primer-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/","title":{"rendered":"Signalizacija FGF pri samoobnovi organoidov raka debelega \u010drevesa"},"content":{"rendered":"

Oblikovanje invalidnih sferoidov in organoidov zaradi inhibicije FGFR v celicah CRC, ki izvirajo od bolnikov, je veliko la\u017eje razumeti z uporabo ilustracij Mind the Graph. <\/p>\n\n\n\n

Shema, ki jo je pripravil avtor, ka\u017ee, da so kirur\u0161ko biopsijo encimsko razgradili, da bi dobili suspenzijo posameznih celic. Naivne nesortirane celice so bile gojene v mediju CSC, ki vsebuje FGF2, ali z zaviralcem FGFR SU-5402.<\/p>\n\n\n\n

Vloga fibroblastnega rastnega dejavnika 2 (FGF2) pri samoobnovi mati\u010dnih celic raka debelega \u010drevesa in danke je bila raziskana<\/strong><\/h2>\n\n\n\n

J\u00f6rg Otte in sodelavci (2019) so v \u010dlanku \"FGF Signalling in the Self-Renewal of Colon Cancer Organoids\" poro\u010dali, da je bila pri naivnih posameznih celicah, vstavljenih v Matrigel in gojenih v CSC-mediju, pri vseh analiziranih bolnikih opa\u017eena kompleksnej\u0161a samoorganizacija. <\/p>\n\n\n\n

Ugotovljeno je bilo, da so v organoidih prekomerno izra\u017eeni geni, ki so neposredno povezani z EMT ter mezenhimskim in samoobnovitvenim fenotipom (TGFB3, ID1, ID2 in ID4). <\/p>\n\n\n\n

Medtem ko je inhibicija FGFR povzro\u010dila okrepljeno signalizacijo MAPK, so opazili inducirano diferenciacijo, izgubo ve\u010dine ozna\u010devalcev mati\u010dnih celic in epitelijski fenotip. <\/p>\n\n\n\n

Rakove mati\u010dne celice so subpopulacija malignih celic, ki se lahko samoobnavljajo in slu\u017eijo kot stalni vir diferenciranih tumorskih celic. V nekaterih protokolih gojenja CSC je pomemben dejavnik rasti fibroblastov 2, ki ohranja mati\u010dnost. <\/p>\n\n\n\n

V organoidih, gojenih v CSC-mediju, pa so zaznali indukcijo TGF-\u03b2 in vseh \u0161tirih \u010dlanov dru\u017eine genov za zaviranje diferenciacije.<\/p>\n\n\n\n

V raziskavo je bilo vklju\u010denih 9 zaporednih neizbranih bolnikov s CRC.<\/strong><\/p>\n\n\n\n

Preverite izvirni \u010dlanek tukaj<\/a>.<\/p>\n\n\n\n

Grafi\u010dni povzetek, ki ga poganja Mind the Graph<\/strong><\/h2>\n\n\n\n
<\/div>\n\n\n
\n
\"\"<\/a>
Izdelano z Mind the Graph<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

Nekatere njihove ugotovitve bi lahko okrepile dosedanje znanje o tem podro\u010dju: \"NANOG, LIN28 in SALL1 so izrazili izklju\u010dno hESC. V na\u0161i kulturi hESC sta bila zaznana GO-termina \"negativna regulacija signalne poti BMP\" in \"prenos signala proteinov SMAD\",\" predlaga Otte.<\/p>\n\n\n\n

Avtorji priznavajo, da je \"analiza GO-term tega nabora genov dala le nekaj pomembnih rezultatov zaradi majhnega \u0161tevila pogosto reguliranih genov. Ve\u010dina teh GO-termov za stanje CSC-medij je bila povezana z aktivno transkripcijo, proliferacijo celic ali pove\u010danim privzemom glukoze. <\/p>\n\n\n\n

Po inhibiciji FGFR smo na\u0161li regulatorje celi\u010dnega cikla in \u0161tevilne gene, anotirane z \"diferenciacijo epitelijskih celic\", \"prebavo\" ali \"transportom \u017eol\u010dnih kislin in \u017eol\u010dnih soli\".\"<\/p>\n\n\n\n

U\u010dinek in uspe\u0161nost dokumentov<\/strong><\/h2>\n\n\n\n

Analiziramo lahko z Altmetric<\/a> podatkov, da je ocena spletne pozornosti tega papirja vi\u0161ja. kot . 68%<\/strong><\/strong> vseh drugih \u010dlankov podobne starosti v reviji Scientific Reports.<\/strong><\/p>\n\n\n\n

<\/div>\n\n\n
\n
\"\"<\/a>
Vir: Altmetrika<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

Izbolj\u0161ajte dostopnost \u010dlanka tudi z infografikami.<\/p>\n\n\n\n

\n
Poskusite Mind the Graph<\/strong><\/a><\/div>\n<\/div>\n\n\n\n
<\/div>","protected":false},"excerpt":{"rendered":"

Ovirano tvorbo sferoidov in organoidov zaradi inhibicije FGFR v celicah CRC, ki izvirajo od bolnikov, je la\u017eje razumeti z na\u0161imi ilustracijami.<\/p>","protected":false},"author":29,"featured_media":20014,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[195,958,957,1],"tags":[96,554,775,963],"yoast_head":"\nFGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog<\/title>\n<meta name=\"description\" content=\"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mindthegraph.com\/blog\/sl\/uspeh-primer-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/\" \/>\n<meta property=\"og:locale\" content=\"sl_SI\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog\" \/>\n<meta property=\"og:description\" content=\"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/mindthegraph.com\/blog\/sl\/uspeh-primer-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/\" \/>\n<meta property=\"og:site_name\" content=\"Mind the Graph Blog\" \/>\n<meta property=\"article:published_time\" content=\"2022-09-10T21:27:00+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2023-03-17T17:47:45+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2022\/09\/blog_4.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1125\" \/>\n\t<meta property=\"og:image:height\" content=\"614\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Artur Zignani\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:image\" content=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2022\/09\/blog_4.jpg\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Artur Zignani\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"3 minutes\" \/>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - 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