{"id":8728,"date":"2019-04-23T16:35:01","date_gmt":"2019-04-23T19:35:01","guid":{"rendered":"https:\/\/blog.mindthegraph.com\/?p=8728"},"modified":"2022-10-18T08:23:59","modified_gmt":"2022-10-18T11:23:59","slug":"visual-abstract-example","status":"publish","type":"post","link":"https:\/\/mindthegraph.com\/blog\/sk\/visual-abstract-example\/","title":{"rendered":"Vizu\u00e1lny abstrakt vytvoren\u00fd pou\u017e\u00edvate\u013eom na vysvetlenie v\u00fdskumnej pr\u00e1ce \"Optimized Small-Molecule Pull-Downs Define MLBP1 as an Acyl-Lipid-Binding Protein\" od Yeleny Sterlin"},"content":{"rendered":"

Vizu\u00e1lnu abstrakciu nie je \u013eahk\u00e9 vytvori\u0165, ale je tu, aby zostala. Preto v\u017edy radi uk\u00e1\u017eeme \u00fa\u017easn\u00e9 pr\u00e1ce na\u0161ich pou\u017e\u00edvate\u013eov ako in\u0161pirat\u00edvny pr\u00edklad vizu\u00e1lnej abstrakcie. Chceme umo\u017eni\u0165 vedcom, aby umo\u017enili vizu\u00e1lnej\u0161iu a dosiahnute\u013enej\u0161iu vedu.<\/p>\n

V pr\u00edspevku v\u00e1m uk\u00e1\u017eem \u010das\u0165 p\u00f4vodn\u00e9ho v\u00fdskumu Yelena Sterlin<\/a> a kolegami. Je doktorandkou na Fakulte po\u013enohospod\u00e1rstva, potravin\u00e1rstva a \u017eivotn\u00e9ho prostredia Hebrejskej univerzity v Jeruzaleme.<\/p>\n

Jej nov\u00fd dokument Optimalizovan\u00e9 Pull-Downs s mal\u00fdmi molekulami definuj\u00fa MLBP1 ako prote\u00edn via\u017euci acyl-lypidy<\/a> <\/strong>vn\u00e1\u0161a svetlo do interakci\u00ed medzi prote\u00ednmi a metabolitmi v rastlin\u00e1ch so zameran\u00edm na receptory kyseliny abscisovej (ABA). Je tie\u017e predplatite\u013eom mind the graph, tak\u017ee vytvorila infografiku v Pozor na graf<\/a> vysvetli\u0165 svoju metodiku.<\/p>\n

V\u00fdznam prote\u00ednov s\u00favisiacich s dom\u00e9nou START<\/strong><\/h2>\n

 <\/p>\n

Pod\u013ea \u010casopis Nature<\/a>, kyselina abscisov\u00e1 (ABA) je hlavn\u00fdm fytohorm\u00f3nom, ktor\u00fd sa podie\u013ea na reakcii na abiotick\u00fd stres a na adapt\u00e1cii na\u0148. Je tie\u017e kandid\u00e1tom na agrochemik\u00e1lie. ABA bola prv\u00fdkr\u00e1t objaven\u00e1 v 60. rokoch 20. storo\u010dia. Potom sa zistilo, \u017ee odvtedy je k\u013e\u00fa\u010dov\u00fdm regul\u00e1torom tak\u00fdch rozmanit\u00fdch procesov, ako je dormancia, kl\u00ed\u010denie, v\u00fdvoj semien a reakcie na biotick\u00fd a abiotick\u00fd stres.<\/p>\n

Najstar\u0161ie udalosti zah\u0155\u0148aj\u00face sign\u00e1lnu dr\u00e1hu ABA zah\u0155\u0148aj\u00fa v\u00e4zbu ABA na receptory PYR\/PYL\/RCARs. Tieto receptory patria do superrodiny s dom\u00e9nou START.<\/p>\n

Prote\u00ednov\u00e9 dom\u00e9ny START s\u00fa dom\u00e9ny via\u017euce lipidy, ktor\u00e9 sa nach\u00e1dzaj\u00fa v gen\u00f3moch bakt\u00e9ri\u00ed, protistov, kvasiniek, rastl\u00edn a \u017eivo\u010d\u00edchov. \u010castej\u0161ie sa v\u0161ak vyskytuj\u00fa v rastlin\u00e1ch. Pod\u013ea Schrick<\/a>, pr\u00edtomnos\u0165 dom\u00e9n START u evolu\u010dne vzdialen\u00fdch druhov, ako s\u00fa \u017eivo\u010d\u00edchy a rastliny, nazna\u010duje konzervovan\u00fd mechanizmus interakcie prote\u00ednov s lipidmi\/sterolmi.<\/p>\n

Dokument uverejnen\u00fd v \u010dasopise Rastlinn\u00fd \u010dasopis<\/a> sa zameriava na receptory ABA PYR\/PYL\/RCAR. V\u00fdskumn\u00edci sa rozhodli optimalizova\u0165 met\u00f3du, ktor\u00e1 by umo\u017enila identifikova\u0165 ligandy START dom\u00e9ny a prote\u00ednov pr\u00edbuzn\u00fdch START dom\u00e9ne v rastlin\u00e1ch, preto\u017ee \"ligandy prote\u00ednov s dom\u00e9nou START s\u00fa nevyhnutn\u00e9 pre spr\u00e1vne fungovanie ich prote\u00ednov u \u017eivo\u010d\u00edchov aj rastl\u00edn a bolo preuk\u00e1zan\u00e9, \u017ee rastlinn\u00e9 prote\u00edny s dom\u00e9nou START sa podie\u013eaj\u00fa na z\u00e1kladn\u00fdch fyziologick\u00fdch procesoch\".<\/em><\/p>\n

Vizu\u00e1lna Abstrakt metodiky<\/strong><\/h2>\n

 <\/p>\n

Na vysvetlenie svojej metodiky vedci vytvorili infografiku s ilustr\u00e1ciami grafov. Je p\u00fatav\u00e1 a \u013eah\u0161ie pochopite\u013en\u00e1. <\/strong><\/h4>\n

 <\/p>\n

Na infografike ni\u017e\u0161ie je zn\u00e1zornen\u00fd experiment\u00e1lny pracovn\u00fd postup. Ako m\u00f4\u017eete vidie\u0165, exprimovali a purifikovali \u0161tyri prote\u00edny START. Supernatant obsahuj\u00faci nat\u00edvnu rozpustn\u00fa frakciu prefiltrovali a inkubovali pomocou streptavid\u00ednovej sefar\u00f3zy, afinitnej \u017eivice na spo\u013eahliv\u00e9 a vysoko-purifik\u00e1cia biomolek\u00fal s vysok\u00fdm rozl\u00ed\u0161en\u00edm. Potom analyzovali alikvoty pomocou western blotu. Na identifik\u00e1ciu ligandu eluovali predpokladan\u00e9 ligandy a podrobili ich anal\u00fdze syst\u00e9mom LC -MS (kvapalinov\u00e1 chromatografia a hmotnostn\u00e1 spektrometria). Molekulov\u00e9 \u0161trukt\u00fary predpokladan\u00fdch ligandov, ktor\u00e9 ich zauj\u00edmali, sa ur\u010dili pomocou tandemovej hmotnostnej spektrometrie.<\/p>\n

 <\/p>\n

\"vizu\u00e1lna<\/p>\n

Autori predstavili dobr\u00fa techniku na po\u010diato\u010dn\u00e9 sk\u00famanie prote\u00ednov\u00fdch ligandov v rastlin\u00e1ch. Pr\u00edstup upravili tak, aby bolo mo\u017en\u00e9 objavi\u0165 START ligandy. Met\u00f3du v\u0161ak mo\u017eno prisp\u00f4sobi\u0165 ligandom r\u00f4znej chemickej povahy \u00fapravou analytick\u00fdch podmienok.<\/p>\n

Ak chcete svoj v\u00fdskum prezentova\u0165 pomocou vedeck\u00fdch ilustr\u00e1ci\u00ed a vizu\u00e1lnych abstraktov, m\u00f4\u017eete pou\u017ei\u0165 aj Mind the Graph. Tak\u017ee ste pripraven\u00ed zlep\u0161i\u0165 svoju komunik\u00e1ciu vo vede?<\/p>\n

\"n\u00e1h\u013ead_250\"<\/a><\/p>","protected":false},"excerpt":{"rendered":"

Vizu\u00e1lnu abstrakciu nie je \u013eahk\u00e9 vytvori\u0165, ale je tu, aby zostala. Preto v\u017edy radi uk\u00e1\u017eeme \u00fa\u017easn\u00e9 pr\u00e1ce na\u0161ich pou\u017e\u00edvate\u013eov ako in\u0161pirat\u00edvny pr\u00edklad vizu\u00e1lnej abstrakcie. Chceme umo\u017eni\u0165 vedcom, aby umo\u017enili vizu\u00e1lnej\u0161iu a dosiahnute\u013enej\u0161iu vedu. V tomto pr\u00edspevku v\u00e1m uk\u00e1\u017eem nie\u010do m\u00e1lo z [...]<\/p>","protected":false},"author":11,"featured_media":8741,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"image","meta":[],"categories":[193,40],"tags":[291,96,292,245,294,295,554,775,293,382],"yoast_head":"\nVisual abstract created by Mind the Graph user. Attractive scientific figure!<\/title>\n<meta name=\"description\" content=\"See the visual abstract created by the MindtheGraph subscriber Yelena Sterling to explain her research in an attractive and instructive way. Awesome job!\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mindthegraph.com\/blog\/sk\/vizualno-abstraktne\/visual-abstract-example\/\" \/>\n<meta property=\"og:locale\" content=\"sk_SK\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Visual abstract created by Mind the Graph user. Attractive scientific figure!\" \/>\n<meta property=\"og:description\" content=\"See the visual abstract created by the MindtheGraph subscriber Yelena Sterling to explain her research in an attractive and instructive way. Awesome job!\" \/>\n<meta property=\"og:url\" content=\"https:\/\/mindthegraph.com\/blog\/sk\/vizualno-abstraktne\/visual-abstract-example\/\" \/>\n<meta property=\"og:site_name\" content=\"Mind the Graph Blog\" \/>\n<meta property=\"article:published_time\" content=\"2019-04-23T19:35:01+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2022-10-18T11:23:59+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2019\/04\/cover-yelena.png\" \/>\n\t<meta property=\"og:image:width\" content=\"622\" \/>\n\t<meta property=\"og:image:height\" content=\"379\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"author\" content=\"Fabiola Soares\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Fabiola Soares\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"3 minutes\" \/>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Visual abstract created by Mind the Graph user. 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