{"id":16636,"date":"2022-09-10T18:27:00","date_gmt":"2022-09-10T21:27:00","guid":{"rendered":"https:\/\/mindthegraph.com\/blog\/?p=16636"},"modified":"2023-03-17T14:47:45","modified_gmt":"2023-03-17T17:47:45","slug":"success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids","status":"publish","type":"post","link":"https:\/\/mindthegraph.com\/blog\/sk\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/","title":{"rendered":"Signaliz\u00e1cia FGF v samoobnove organoidov rakoviny hrub\u00e9ho \u010dreva"},"content":{"rendered":"

Tvorba postihnut\u00fdch sf\u00e9roidov a organoidov inhib\u00edciou FGFR v bunk\u00e1ch CRC odvoden\u00fdch od pacienta je ove\u013ea \u013eah\u0161ie pochopite\u013en\u00e1 pomocou ilustr\u00e1ci\u00ed Mind the Graph. <\/p>\n\n\n\n

Sch\u00e9ma vytvoren\u00e1 autorom ukazuje, \u017ee chirurgick\u00e9 biopsie boli enzymaticky rozlo\u017een\u00e9, aby sa z\u00edskala suspenzia jednotliv\u00fdch buniek. Naivn\u00e9 netrieden\u00e9 bunky sa kultivovali v m\u00e9diu CSC obsahuj\u00facom FGF2 alebo s inhib\u00edtorom FGFR SU-5402.<\/p>\n\n\n\n

Sk\u00famala sa \u00faloha fibroblastov\u00e9ho rastov\u00e9ho faktora 2 (FGF2) pri samoobnove kme\u0148ov\u00fdch buniek kolorekt\u00e1lneho karcin\u00f3mu<\/strong><\/h2>\n\n\n\n

V pr\u00e1ci \"FGF Signalling in the Self-Renewal of Colon Cancer Organoids\" (Signaliz\u00e1cia FGF v samoobnove organoidov rakoviny hrub\u00e9ho \u010dreva) J\u00f6rg Otte a jeho kolegovia (2019) uviedli, \u017ee ke\u010f boli naivn\u00e9 jednotliv\u00e9 bunky vlo\u017een\u00e9 do Matrigelu a kultivovan\u00e9 v CSC-m\u00e9diu, u v\u0161etk\u00fdch analyzovan\u00fdch pacientov sa pozorovala komplexnej\u0161ia samoorganiz\u00e1cia. <\/p>\n\n\n\n

Zistilo sa, \u017ee g\u00e9ny priamo spojen\u00e9 s EMT spolu s mezench\u00fdmov\u00fdm a samoobnovuj\u00facim sa fenotypom (TGFB3, ID1, ID2 a ID4) s\u00fa v organoidoch nadmerne exprimovan\u00e9. <\/p>\n\n\n\n

Zatia\u013e \u010do inhib\u00edcia FGFR viedla k zv\u00fd\u0161enej signaliz\u00e1cii MAPK, pozorovali indukovan\u00fa diferenci\u00e1ciu, stratu v\u00e4\u010d\u0161iny markerov kme\u0148ov\u00fdch buniek a epitelov\u00fd fenotyp. <\/p>\n\n\n\n

N\u00e1dorov\u00e9 kme\u0148ov\u00e9 bunky s\u00fa subpopul\u00e1ciou mal\u00edgnych buniek schopn\u00fdch samoobnovy a sl\u00fa\u017eia ako trval\u00fd zdroj diferencovan\u00fdch n\u00e1dorov\u00fdch buniek. V niektor\u00fdch protokoloch kultiv\u00e1cie CSC je fibroblastov\u00fd rastov\u00fd faktor 2 d\u00f4le\u017eitou zlo\u017ekou na udr\u017eanie kme\u0148ovitosti. <\/p>\n\n\n\n

Na druhej strane v organoidoch kultivovan\u00fdch na CSC m\u00e9diu zistili indukciu TGF-\u03b2 a v\u0161etk\u00fdch \u0161tyroch \u010dlenov rodiny g\u00e9nov inhib\u00edtora diferenci\u00e1cie.<\/p>\n\n\n\n

Do v\u00fdskumu bolo zapojen\u00fdch 9 po sebe nasleduj\u00facich nevybran\u00fdch pacientov s CRC.<\/strong><\/p>\n\n\n\n

Skontrolujte p\u00f4vodn\u00fd \u010dl\u00e1nok tu<\/a>.<\/p>\n\n\n\n

Grafick\u00e9 abstraktum poh\u00e1\u0148an\u00e9 Mind the Graph<\/strong><\/h2>\n\n\n\n
<\/div>\n\n\n
\n
\"\"<\/a>
Vyroben\u00e9 s Mind the Graph<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

Niektor\u00e9 z ich z\u00e1verov potenci\u00e1lne posil\u0148uj\u00fa doteraj\u0161ie poznatky o tejto oblasti: \"G\u00e9ny s\u00favisiace s pluripotenciou NANOG, LIN28 a SALL1 boli exprimovan\u00e9 v\u00fdlu\u010dne hESC. V na\u0161ej kult\u00fare hESC boli zisten\u00e9 GO-term\u00edny \"negat\u00edvna regul\u00e1cia sign\u00e1lnej dr\u00e1hy BMP\" a \"prenos sign\u00e1lu prote\u00ednu SMAD\",\" nazna\u010dil Otte.<\/p>\n\n\n\n

Autori prizn\u00e1vaj\u00fa, \u017ee \"GO-term anal\u00fdza tohto s\u00faboru g\u00e9nov priniesla len nieko\u013eko v\u00fdznamn\u00fdch v\u00fdsledkov kv\u00f4li n\u00edzkemu po\u010dtu be\u017ene regulovan\u00fdch g\u00e9nov. V\u00e4\u010d\u0161ina t\u00fdchto GO-termov pre stav CSC-medium bola spojen\u00e1 s akt\u00edvnou transkripciou, prolifer\u00e1ciou buniek alebo zv\u00fd\u0161en\u00fdm pr\u00edjmom gluk\u00f3zy. <\/p>\n\n\n\n

Po inhib\u00edcii FGFR sme na\u0161li regul\u00e1tory bunkov\u00e9ho cyklu, ako aj mnoh\u00e9 g\u00e9ny anotovan\u00e9 ako \"diferenci\u00e1cia epitelov\u00fdch buniek\", \"tr\u00e1venie\" alebo \"transport \u017el\u010dov\u00fdch kysel\u00edn a \u017el\u010dov\u00fdch sol\u00ed\".\"<\/p>\n\n\n\n

Dokumenty Vplyv a v\u00fdkon<\/strong><\/h2>\n\n\n\n

M\u00f4\u017eeme analyzova\u0165 pomocou Altmetric<\/a> \u00fadaje, \u017ee sk\u00f3re online pozornosti tohto dokumentu je vy\u0161\u0161ie ako . 68%<\/strong><\/strong> v\u0161etk\u00fdch ostatn\u00fdch \u010dl\u00e1nkov podobn\u00e9ho veku v \u010dasopise Scientific Reports.<\/strong><\/p>\n\n\n\n

<\/div>\n\n\n
\n
\"\"<\/a>
Zdroj Altmetrika<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

Zlep\u0161ite pr\u00edstupnos\u0165 svojho \u010dl\u00e1nku aj pomocou infografiky.<\/p>\n\n\n\n

\n
Vysk\u00fa\u0161ajte Mind the Graph<\/strong><\/a><\/div>\n<\/div>\n\n\n\n
<\/div>","protected":false},"excerpt":{"rendered":"

Inhib\u00edciou FGFR v bunk\u00e1ch CRC odvoden\u00fdch od pacienta je mo\u017en\u00e9 \u013eah\u0161ie pochopi\u0165 tvorbu postihnut\u00fdch sf\u00e9roidov a organoidov pomocou na\u0161ich ilustr\u00e1ci\u00ed.<\/p>","protected":false},"author":29,"featured_media":20014,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[195,958,957,1],"tags":[96,554,775,963],"yoast_head":"\nFGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog<\/title>\n<meta name=\"description\" content=\"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mindthegraph.com\/blog\/sk\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/\" \/>\n<meta property=\"og:locale\" content=\"sk_SK\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog\" \/>\n<meta property=\"og:description\" content=\"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/mindthegraph.com\/blog\/sk\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/\" \/>\n<meta property=\"og:site_name\" content=\"Mind the Graph Blog\" \/>\n<meta property=\"article:published_time\" content=\"2022-09-10T21:27:00+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2023-03-17T17:47:45+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2022\/09\/blog_4.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1125\" \/>\n\t<meta property=\"og:image:height\" content=\"614\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Artur Zignani\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:image\" content=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2022\/09\/blog_4.jpg\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Artur Zignani\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"3 minutes\" \/>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog","description":"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/mindthegraph.com\/blog\/sk\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/","og_locale":"sk_SK","og_type":"article","og_title":"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog","og_description":"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.","og_url":"https:\/\/mindthegraph.com\/blog\/sk\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/","og_site_name":"Mind the Graph Blog","article_published_time":"2022-09-10T21:27:00+00:00","article_modified_time":"2023-03-17T17:47:45+00:00","og_image":[{"width":1125,"height":614,"url":"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2022\/09\/blog_4.jpg","type":"image\/jpeg"}],"author":"Artur Zignani","twitter_card":"summary_large_image","twitter_image":"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2022\/09\/blog_4.jpg","twitter_misc":{"Written by":"Artur Zignani","Est. reading time":"3 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/mindthegraph.com\/blog\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/","url":"https:\/\/mindthegraph.com\/blog\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/","name":"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog","isPartOf":{"@id":"https:\/\/mindthegraph.com\/blog\/#website"},"datePublished":"2022-09-10T21:27:00+00:00","dateModified":"2023-03-17T17:47:45+00:00","author":{"@id":"https:\/\/mindthegraph.com\/blog\/#\/schema\/person\/b44995c1d77b52a63b6d11262b740b02"},"description":"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.","breadcrumb":{"@id":"https:\/\/mindthegraph.com\/blog\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/#breadcrumb"},"inLanguage":"sk-SK","potentialAction":[{"@type":"ReadAction","target":["https:\/\/mindthegraph.com\/blog\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/mindthegraph.com\/blog\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/mindthegraph.com\/blog\/"},{"@type":"ListItem","position":2,"name":"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review"}]},{"@type":"WebSite","@id":"https:\/\/mindthegraph.com\/blog\/#website","url":"https:\/\/mindthegraph.com\/blog\/","name":"Mind the Graph Blog","description":"Your science can be beautiful!","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/mindthegraph.com\/blog\/?s={search_term_string}"},"query-input":"required name=search_term_string"}],"inLanguage":"sk-SK"},{"@type":"Person","@id":"https:\/\/mindthegraph.com\/blog\/#\/schema\/person\/b44995c1d77b52a63b6d11262b740b02","name":"Artur Zignani","image":{"@type":"ImageObject","inLanguage":"sk-SK","@id":"https:\/\/mindthegraph.com\/blog\/#\/schema\/person\/image\/","url":"https:\/\/secure.gravatar.com\/avatar\/a211ec97011fe24f7a8e63a7ca799342?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/a211ec97011fe24f7a8e63a7ca799342?s=96&d=mm&r=g","caption":"Artur Zignani"},"url":"https:\/\/mindthegraph.com\/blog\/sk\/author\/artur\/"}]}},"_links":{"self":[{"href":"https:\/\/mindthegraph.com\/blog\/sk\/wp-json\/wp\/v2\/posts\/16636"}],"collection":[{"href":"https:\/\/mindthegraph.com\/blog\/sk\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/mindthegraph.com\/blog\/sk\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/mindthegraph.com\/blog\/sk\/wp-json\/wp\/v2\/users\/29"}],"replies":[{"embeddable":true,"href":"https:\/\/mindthegraph.com\/blog\/sk\/wp-json\/wp\/v2\/comments?post=16636"}],"version-history":[{"count":5,"href":"https:\/\/mindthegraph.com\/blog\/sk\/wp-json\/wp\/v2\/posts\/16636\/revisions"}],"predecessor-version":[{"id":27203,"href":"https:\/\/mindthegraph.com\/blog\/sk\/wp-json\/wp\/v2\/posts\/16636\/revisions\/27203"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/mindthegraph.com\/blog\/sk\/wp-json\/wp\/v2\/media\/20014"}],"wp:attachment":[{"href":"https:\/\/mindthegraph.com\/blog\/sk\/wp-json\/wp\/v2\/media?parent=16636"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/mindthegraph.com\/blog\/sk\/wp-json\/wp\/v2\/categories?post=16636"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/mindthegraph.com\/blog\/sk\/wp-json\/wp\/v2\/tags?post=16636"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}