{"id":8728,"date":"2019-04-23T16:35:01","date_gmt":"2019-04-23T19:35:01","guid":{"rendered":"https:\/\/blog.mindthegraph.com\/?p=8728"},"modified":"2022-10-18T08:23:59","modified_gmt":"2022-10-18T11:23:59","slug":"visual-abstract-example","status":"publish","type":"post","link":"https:\/\/mindthegraph.com\/blog\/ro\/vizual-abstract-exemplu\/","title":{"rendered":"Rezumat vizual creat de utilizator pentru a explica lucrarea de cercetare \"Optimized Small-Molecule Pull-Downs Define MLBP1 as an Acyl-Lipid-Binding Protein\" de Yelena Sterlin"},"content":{"rendered":"

Un abstract vizual nu este u\u0219or de creat, dar este aici pentru a r\u0103m\u00e2ne. A\u0219a c\u0103 ne place \u00eentotdeauna s\u0103 prezent\u0103m munca uimitoare a utilizatorilor no\u0219tri ca exemplu inspirat de abstract vizual. Dorim s\u0103 le d\u0103m oamenilor de \u0219tiin\u021b\u0103 posibilitatea de a permite o \u0219tiin\u021b\u0103 mai vizual\u0103 \u0219i mai accesibil\u0103.<\/p>\n

\u00cen postare, v\u0103 voi ar\u0103ta un pic din cercetarea original\u0103 de c\u0103tre Yelena Sterlin<\/a> \u0219i colegii. Este doctorand\u0103 la Facultatea de Agricultur\u0103, Alimenta\u021bie \u0219i Mediu din cadrul Universit\u0103\u021bii Ebraice din Ierusalim.<\/p>\n

Noua ei lucrare Optimizarea extrac\u021biilor cu molecule mici define\u0219te MLBP1 ca o protein\u0103 de legare a acilo-lipidelor<\/a> <\/strong>pune \u00een lumin\u0103 interac\u021biunile dintre proteine \u0219i metaboli\u021bi \u00een plante, cu accent pe receptorii acidului abscisic (ABA). Ea este, de asemenea, abonat\u0103 la mind the graph, a\u0219a c\u0103 a creat un infografic \u00een aten\u021bie la grafic<\/a> pentru a-\u0219i explica metodologia.<\/p>\n

Importan\u021ba proteinelor legate de domeniul START<\/strong><\/h2>\n

 <\/p>\n

Potrivit Revista Nature<\/a>, acidul abscisic (ABA) este principalul fitohormon implicat \u00een r\u0103spunsul la stresul abiotic \u0219i \u00een adaptarea acestuia. De asemenea, este un produs agricol candidat. ABA a fost descoperit pentru prima dat\u0103 \u00een anii 1960. Dup\u0103 aceea, s-a constatat c\u0103 este un regulator cheie al unor procese diverse, cum ar fi dorman\u021ba, germina\u021bia, dezvoltarea semin\u021belor \u0219i r\u0103spunsurile la stresul biotic \u0219i abiotic.<\/p>\n

Cele mai timpurii evenimente care implic\u0103 calea de semnalizare ABA implic\u0103 legarea ABA de receptorii PYR\/PYL\/RCARs. Ace\u0219ti receptori apar\u021bin superfamiliei START-domain.<\/p>\n

Domeniile proteice START sunt un domeniu de legare a lipidelor prezent \u00een genomurile de bacterii, proti\u0219ti, drojdii, plante \u0219i animale. Cu toate acestea, sunt mai frecvente \u00een cazul plantelor. Conform Schrick<\/a>, prezen\u021ba domeniilor START \u00een specii \u00eendep\u0103rtate din punct de vedere evolutiv, cum ar fi animalele \u0219i plantele, sugereaz\u0103 un mecanism conservat de interac\u021biune a proteinelor cu lipidele\/sterolii.<\/p>\n

Lucrarea publicat\u0103 \u00een Jurnalul de plante<\/a> se concentreaz\u0103 asupra receptorilor ABA PYR\/PYL\/RCARs. Cercet\u0103torii au decis s\u0103 optimizeze o metod\u0103 care s\u0103 permit\u0103 identificarea liganzilor proteinelor cu domeniu START \u0219i a celor legate de domeniul START \u00een plante, deoarece \"liganzii proteinei de domeniu START sunt indispensabili pentru buna func\u021bionare a proteinei lor at\u00e2t la animale, c\u00e2t \u0219i la plante, iar proteinele de domeniu START din plante s-a demonstrat c\u0103 sunt implicate \u00een procese fiziologice esen\u021biale\".<\/em><\/p>\n

Un rezumat vizual al metodologiei<\/strong><\/h2>\n

 <\/p>\n

Pentru a-\u0219i explica metodologia, cercet\u0103torii au creat un infografic cu ilustra\u021bii grafice. Este atr\u0103g\u0103tor \u0219i mai u\u0219or de \u00een\u021beles. <\/strong><\/h4>\n

 <\/p>\n

Infograficul de mai jos prezint\u0103 fluxul de lucru experimental. Dup\u0103 cum pute\u021bi vedea, au exprimat \u0219i purificat patru proteine START. Au filtrat \u0219i incubat supernatantul care con\u021binea frac\u021biunea solubil\u0103 nativ\u0103 folosind streptavidin\u0103 sefarazei, o r\u0103\u0219in\u0103 de afinitate pentru o reac\u021bie fiabil\u0103 \u0219i de \u00eenalt\u0103 calitate.-purificarea cu rezolu\u021bie a biomoleculelor. Apoi, ei au analizat alicoturile cu ajutorul Western Blot. Pentru a identifica ligandul, ei au eluat liganzi putativi \u0219i au supus o analiz\u0103 de sistem LC -MS (cromatografie lichid\u0103 \u0219i spectrometrie de mas\u0103). Structurile moleculare ale liganzilor putativi de interes au fost determinate prin spectrometrie de mas\u0103 \u00een tandem.<\/p>\n

 <\/p>\n

\"abstract<\/p>\n

Autorii au prezentat o tehnic\u0103 bun\u0103 pentru sondarea ini\u021bial\u0103 a liganzilor proteici \u00een plante. Ei au ajustat abordarea pentru a descoperi liganzi START. Cu toate acestea, pute\u021bi adopta metoda la liganzi de natur\u0103 chimic\u0103 diferit\u0103 prin ajustarea condi\u021biilor analitice.<\/p>\n

Dac\u0103 dori\u021bi s\u0103 v\u0103 prezenta\u021bi cercet\u0103rile folosind ilustra\u021bii \u0219tiin\u021bifice \u0219i rezumate vizuale, pute\u021bi utiliza \u0219i Mind the Graph. A\u0219adar, sunte\u021bi gata s\u0103 v\u0103 \u00eembun\u0103t\u0103\u021bi\u021bi comunicarea \u00een domeniul \u0219tiin\u021bei?<\/p>\n

\"previzualizare_250\"<\/a><\/p>","protected":false},"excerpt":{"rendered":"

Un abstract vizual nu este u\u0219or de creat, dar este aici pentru a r\u0103m\u00e2ne. A\u0219a c\u0103 ne place \u00eentotdeauna s\u0103 prezent\u0103m munca uimitoare a utilizatorilor no\u0219tri ca exemplu inspirat de abstract vizual. Dorim s\u0103 le d\u0103m oamenilor de \u0219tiin\u021b\u0103 posibilitatea de a permite o \u0219tiin\u021b\u0103 mai vizual\u0103 \u0219i mai accesibil\u0103. \u00cen aceast\u0103 postare, v\u0103 voi ar\u0103ta un pic din [...]<\/p>","protected":false},"author":11,"featured_media":8741,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"image","meta":[],"categories":[193,40],"tags":[291,96,292,245,294,295,554,775,293,382],"yoast_head":"\nVisual abstract created by Mind the Graph user. Attractive scientific figure!<\/title>\n<meta name=\"description\" content=\"See the visual abstract created by the MindtheGraph subscriber Yelena Sterling to explain her research in an attractive and instructive way. 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