{"id":16636,"date":"2022-09-10T18:27:00","date_gmt":"2022-09-10T21:27:00","guid":{"rendered":"https:\/\/mindthegraph.com\/blog\/?p=16636"},"modified":"2023-03-17T14:47:45","modified_gmt":"2023-03-17T17:47:45","slug":"success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids","status":"publish","type":"post","link":"https:\/\/mindthegraph.com\/blog\/ro\/succes-caz-fgf-semnalizare-fgf-in-innoirea-intregii-organoizi-canceroase-de-colon\/","title":{"rendered":"Semnalizarea FGF \u00een auto-re\u00eennoirea organoizilor de cancer de colon Recenzie de h\u00e2rtie"},"content":{"rendered":"

Formarea de sferoizi \u0219i organoizi dezactiva\u021bi prin inhibarea FGFR \u00een celulele de CCR provenite de la pacien\u021bi este mult mai u\u0219or de \u00een\u021beles cu ajutorul ilustra\u021biilor Mind the Graph. <\/p>\n\n\n\n

Schema creat\u0103 de autor arat\u0103 c\u0103 biopsiile chirurgicale au fost digerate enzimatic pentru a ob\u021bine o suspensie de celule unice. Celulele naive nesortate au fost cultivate \u00een mediu CSC-mediu con\u021bin\u00e2nd FGF2 sau cu inhibitorul FGFR SU-5402.<\/p>\n\n\n\n

Rolul factorului de cre\u0219tere a fibroblastului 2 (FGF2) \u00een auto-re\u00eennoirea celulelor stem ale cancerului colorectal a fost investigat.<\/strong><\/h2>\n\n\n\n

\u00cen \"FGF Signalling in the Self-Renewal of Colon Cancer Organoids\", J\u00f6rg Otte \u0219i colegii s\u0103i (2019) au raportat c\u0103, atunci c\u00e2nd celulele unice naive au fost \u00eencorporate \u00een Matrigel \u0219i cultivate \u00een mediul CSC, s-a observat o auto-organizare mai complex\u0103 la to\u021bi pacien\u021bii analiza\u021bi. <\/p>\n\n\n\n

S-a constatat c\u0103 genele direct asociate cu EMT \u00eempreun\u0103 cu un fenotip mezenchimal \u0219i de auto-re\u00eennoire (TGFB3, ID1, ID2 \u0219i ID4) au fost supraexprimate \u00een organoizi. <\/p>\n\n\n\n

\u00cen timp ce inhibarea FGFR a dus la o semnalizare MAPK \u00eembun\u0103t\u0103\u021bit\u0103, au observat o diferen\u021biere indus\u0103, o pierdere a majorit\u0103\u021bii markerilor celulelor stem \u0219i un fenotip epitelial. <\/p>\n\n\n\n

Celulele stem canceroase sunt o subpopula\u021bie de celule maligne capabile s\u0103 se auto-re\u00eennoiasc\u0103 \u0219i s\u0103 serveasc\u0103 drept surs\u0103 continu\u0103 de celule tumorale diferen\u021biate. \u00cen unele protocoale de cultivare a CSC, factorul de cre\u0219tere a fibroblastelor 2 este un ingredient important pentru men\u021binerea caracterului stem. <\/p>\n\n\n\n

Pe de alt\u0103 parte, \u00een organoidele cultivate cu mediu CSC, au detectat inducerea TGF-\u03b2 \u0219i a tuturor celor patru membri ai familiei de gene inhibitoare de diferen\u021biere.<\/p>\n\n\n\n

\u00cen cercetare au fost implica\u021bi 9 pacien\u021bi consecutivi, neselecta\u021bi, cu CCR.<\/strong><\/p>\n\n\n\n

Verifica\u021bi articolul original aici<\/a>.<\/p>\n\n\n\n

Rezumat grafic powered by Mind the Graph<\/strong><\/h2>\n\n\n\n
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Fabricat cu Mind the Graph<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

Unele dintre concluziile lor ar putea consolida ceea ce se \u0219tia anterior despre acest domeniu: \"Genele asociate pluripoten\u021bei NANOG, LIN28 \u0219i SALL1 au fost exprimate exclusiv de hESC. Termenii GO \"reglarea negativ\u0103 a c\u0103ii de semnalizare BMP\" \u0219i \"transduc\u021bia semnalului proteinei SMAD\" au fost detecta\u021bi \u00een cultura noastr\u0103 de hESC\", a sugerat Otte.<\/p>\n\n\n\n

Autorii recunosc c\u0103 \"analiza GO-term a acestui set de gene a dat doar c\u00e2teva rezultate semnificative din cauza num\u0103rului redus de gene reglementate \u00een mod obi\u0219nuit. Majoritatea acestor GO-termeni pentru starea CSC-mediu au fost asociate cu transcrip\u021bia activ\u0103, proliferarea celular\u0103 sau cre\u0219terea absorb\u021biei de glucoz\u0103. <\/p>\n\n\n\n

Dup\u0103 inhibarea FGFR, am g\u0103sit regulatori ai ciclului celular, precum \u0219i multe gene adnotate cu \"diferen\u021bierea celulelor epiteliale\", \"digestia\" sau \"transportul acizilor biliari \u0219i al s\u0103rurilor biliare\".\"<\/p>\n\n\n\n

Lucr\u0103ri Impact \u0219i performan\u021b\u0103<\/strong><\/h2>\n\n\n\n

Putem analiza cu Altmetric<\/a> date c\u0103 scorul de aten\u021bie online al acestei lucr\u0103ri este mai mare dec\u00e2t 68%<\/strong><\/strong> din toate celelalte articole de o v\u00e2rst\u0103 similar\u0103 din Scientific Reports.<\/strong><\/p>\n\n\n\n

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Sursa Altmetrics<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

\u00cembun\u0103t\u0103\u021bi\u021bi accesibilitatea articolului dvs. \u0219i cu ajutorul infograficelor.<\/p>\n\n\n\n

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\u00cencerca\u021bi Mind the Graph<\/strong><\/a><\/div>\n<\/div>\n\n\n\n
<\/div>","protected":false},"excerpt":{"rendered":"

Formarea sferoidelor \u0219i a organoizilor dezactivate prin inhibarea FGFR \u00een celulele CRC provenite de la pacien\u021bi sunt mai u\u0219or de \u00een\u021beles cu ajutorul ilustra\u021biilor noastre.<\/p>","protected":false},"author":29,"featured_media":20014,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[195,958,957,1],"tags":[96,554,775,963],"yoast_head":"\nFGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog<\/title>\n<meta name=\"description\" content=\"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mindthegraph.com\/blog\/ro\/succes-caz-fgf-semnalizare-fgf-in-innoirea-intregii-organoizi-canceroase-de-colon\/\" \/>\n<meta property=\"og:locale\" content=\"ro_RO\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - 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