{"id":12130,"date":"2021-01-13T09:30:00","date_gmt":"2021-01-13T11:30:00","guid":{"rendered":"https:\/\/mindthegraph.com\/blog\/?p=12130"},"modified":"2022-10-18T08:09:26","modified_gmt":"2022-10-18T11:09:26","slug":"pyscratch-spatial-and-temporal-information-from-scratch-assays","status":"publish","type":"post","link":"https:\/\/mindthegraph.com\/blog\/ro\/pyscratch-spatial-and-temporal-information-from-scratch-assays\/","title":{"rendered":"PyScratch: Un nou instrument pentru procesarea informa\u021biilor spa\u021biale \u0219i temporale din testele Scratch"},"content":{"rendered":"

PyScratch este un nou software open-source implementat \u00een Python pentru analiza analizelor de date de migrare, cu o interfa\u021b\u0103 prietenoas\u0103 care permite utilizarea sa de c\u0103tre oamenii de \u0219tiin\u021b\u0103 cu pu\u021bine sau deloc cuno\u0219tin\u021be de programare. <\/p>\n\n\n\n

Software-ul a fost conceput \u00een cadrul unui parteneriat \u00eentre trei oameni de \u0219tiin\u021b\u0103 brazilieni din laboratorul NanoCell Interactions de la Universitatea din Campinas \u00een 2017. <\/p>\n\n\n\n

Acesta s-a n\u0103scut din necesitatea acestor oameni de \u0219tiin\u021b\u0103, iar ast\u0103zi scopul software-ului este de a facilita practica zilnic\u0103 a cercet\u0103torilor, de a exclude analiza manual\u0103, de a cre\u0219te reproductibilitatea \u0219i de a minimiza erorile umane.<\/p>\n\n\n\n

\"\"<\/a><\/figure><\/div>\n\n\n\n

Analiza imaginilor a fost una dintre cele mai importante metode utilizate de oamenii de \u0219tiin\u021b\u0103 \u00een diferite metodologii pentru a analiza rezultatele. <\/p>\n\n\n\n

Nu numai utilizarea microscoapelor automate a crescut \u00een ultimii ani, ci \u0219i complexitatea datelor ob\u021binute. <\/p>\n\n\n\n

O parte din munca unui om de \u0219tiin\u021b\u0103 const\u0103 \u00een a descoperi cum s\u0103 se ocupe de un nou tip de informa\u021bii, \u00een afar\u0103 de a analiza \u0219i procesa datele. <\/p>\n\n\n\n

Pentru ca acest lucru s\u0103 se \u00eent\u00e2mple, oamenii de \u0219tiin\u021b\u0103 au nevoie de instrumente bune \u0219i specializate pentru a extrage \u0219i interpreta corect toate datele.<\/p>\n\n\n\n

PyScratch a fost creat ini\u021bial pentru a o ajuta pe Fernanda Garcia-Fossa, un cercet\u0103tor biolog, s\u0103 analizeze o cantitate imens\u0103 de date din testele sale de migrare. <\/p>\n\n\n\n

\"Am realizat scratch-ul celulelor canceroase \u0219i am incubat \u00een echipament timp de 48 de ore, achizi\u021bion\u00e2nd imagini la fiecare 15 minute, iar la finalul unui singur experiment, aveam aproximativ o mie de imagini pe care s\u0103 le privesc \u0219i s\u0103 le analizez! <\/p>\n\n\n\n

Era imposibil s\u0103 o facem manual\", spune Garcia-Fossa. Pentru a rezolva aceast\u0103 problem\u0103, Garcia-Fossa a apelat la ajutorul partenerului ei, fizicianul Vladimir Gaal, care \u00een acea perioad\u0103 \u00eenv\u0103\u021ba Python, ceea ce a fost o ocazie excelent\u0103 de a pune \u00een practic\u0103 aceste cuno\u0219tin\u021be.<\/p>\n\n\n\n

Astfel, ambii au lucrat \u00een solu\u021bie printr-o rutin\u0103 Python, care a recunoscut zonele zg\u00e2riate \u0219i a exportat \u00eentr-un fi\u0219ier csv. <\/p>\n\n\n\n

\"Cu timpul, am sim\u021bit necesitatea de a dezvolta o interfa\u021b\u0103 de utilizator care s\u0103 fie \u0219i mai u\u0219or de utilizat, \u00een acest fel am putut publica software-ul pentru orice cercet\u0103tor care avea nevoie s\u0103 \u00eel foloseasc\u0103\", spune Garcia-Fossa despre articolul despre software pe care \u00eel pute\u021bi verifica. f\u0103c\u00e2nd clic aici<\/a>.<\/p>\n\n\n\n

Garcia-Fossa spune, de asemenea, c\u0103 a fost nevoie de ceva timp pentru a recunoa\u0219te \u0219i a defini zona de migra\u021bie din imagini, deoarece imaginile pot fi foarte diferite una de alta din cauza luminii, a focaliz\u0103rii \u0219i a contrastului, iar versiunea folosit\u0103 ast\u0103zi poate deja s\u0103 analizeze destul de bine. <\/p>\n\n\n\n

Cu toate acestea, \u00eenc\u0103 se lucreaz\u0103 la lansarea unor versiuni noi \u0219i mai bune ale software-ului, deoarece articolul publicat a scos la iveal\u0103 unele nevoi de \u00eembun\u0103t\u0103\u021bire din cauza cererii utilizatorilor.<\/p>\n\n\n\n

Testul utilizat pentru a valida performan\u021ba software-ului, testul de migrare, testul de zg\u00e2riere sau de vindecare a r\u0103nilor, este un test utilizat \u00een mod obi\u0219nuit \u00een biologie, deoarece permite analiza mecanismului care st\u0103 la baza evenimentelor celulare fiziologice \u0219i patologice. <\/p>\n\n\n\n

Studiul vindec\u0103rii r\u0103nilor este o modalitate important\u0103 de a \u00een\u021belege dezvoltarea \u0219i modelarea \u021besuturilor, pe l\u00e2ng\u0103 angiogenez\u0103 \u0219i dezvoltarea tumorilor. <\/p>\n\n\n\n

Atunci c\u00e2nd testul este efectuat \u00een dou\u0103 dimensiuni, este posibil s\u0103 se m\u0103soare c\u00e2t de repede reac\u021bioneaz\u0103 celulele la ran\u0103, acoperind zona definit\u0103.<\/p>\n\n\n\n

Cu alte cuvinte, experimentul const\u0103, \u00een esen\u021b\u0103, \u00een crearea unui gol \u00een monostrat de celule de confluen\u021b\u0103 \u00eentr-o plac\u0103. <\/p>\n\n\n\n

\u00cen timp, pentru a umple golul, celulele \u00eencep s\u0103 migreze, iar viteza de migrare a celulelor poate fi m\u0103surat\u0103. <\/p>\n\n\n\n

Apoi, pentru a m\u0103sura viteza de migrare a celulelor, trebuie s\u0103 achizi\u021bioneze o mul\u021bime de imagini, ceea ce, la r\u00e2ndul s\u0103u, reprezint\u0103 o etap\u0103 problematic\u0103 a analizei, deoarece necesit\u0103 m\u0103sur\u0103tori manuale. <\/p>\n\n\n\n

Din fericire, ast\u0103zi avem la dispozi\u021bie o mul\u021bime de tehnologii pentru a ne \u00eembun\u0103t\u0103\u021bi \u0219i moderniza procesul de analiz\u0103, permi\u021b\u00e2ndu-le oamenilor de \u0219tiin\u021b\u0103 s\u0103 adopte modalit\u0103\u021bi mai bune \u0219i mai personalizate de a ob\u021bine un rezultat. <\/p>\n\n\n\n

A\u0219a cum au f\u0103cut-o Garcia-Fossa \u0219i Gaal.<\/p>\n\n\n\n

Ast\u0103zi este posibil s\u0103 se g\u0103seasc\u0103 \u0219i alte instrumente comerciale \u0219i necomerciale disponibile pentru a prelucra zona r\u0103nilor. <\/p>\n\n\n\n

Dar ele nu sunt at\u00e2t de simple ca PyScratch \u0219i necesit\u0103 din partea utilizatorului un anumit nivel de programare \u0219i, de asemenea, necesit\u0103 o aten\u021bie permanent\u0103 din partea utilizatorului, ceea ce face ca analiza s\u0103 fie mai susceptibil\u0103 la erori umane, la care se adaug\u0103 timpul de care are nevoie cercet\u0103torul pentru a analiza toate imaginile \u0219i datele.<\/p>\n\n\n\n

\u00cen articol, autorii explic\u0103 modul \u00een care func\u021bioneaz\u0103 acest software. Din toate imaginile realizate \u00een cadrul experimentului, utilizatorul ob\u021bine un fi\u0219ier cu valori separate prin virgul\u0103 (.cvs), un output care stocheaz\u0103 date tabelare \u00een text simplu. <\/p>\n\n\n\n

Apoi, utilizatorul poate prelucra datele \u00een cadrul rutinei sale obi\u0219nuite. Garcia-Fossa spune c\u0103 programul a fost esen\u021bial pentru teza ei de masterat: \"Software-ul transform\u0103 datele de intrare \u00een valori care au sens biologic, cum ar fi viteza de migrare a celulelor. <\/p>\n\n\n\n

Am putut s\u0103 analizez mai bine efectul nanoparticulei mele asupra celulelor canceroase de prostat\u0103 \u0219i s\u0103 m\u0103sor viteza de migrare a celulelor, precum \u0219i timpul exact pentru \u00eenchiderea bun\u0103, toate acestea datorit\u0103 PyScratch.\"<\/p>\n\n\n\n

\"\"<\/a><\/figure><\/div>\n\n\n\n

Dac\u0103 dori\u021bi s\u0103 \u00eencerca\u021bi PyScratch pentru cercet\u0103rile dumneavoastr\u0103, software-ul este disponibil \u00een mod gratuit \u0219i oricine din comunitatea \u0219tiin\u021bific\u0103 are voie s\u0103 \u00eel foloseasc\u0103, ajut\u00e2ndu-i pe Garcia-Fossa \u0219i Gaal s\u0103 \u00eembun\u0103t\u0103\u021beasc\u0103 \u0219i s\u0103 perfec\u021bioneze programul.<\/p>\n\n\n\n

 ____<\/p>\n\n\n\n

Cum decurge experien\u021ba ta Mind the Graph p\u00e2n\u0103 acum? Ajuta\u021bi-ne s\u0103 \u00eembun\u0103t\u0103\u021bim platforma noastr\u0103 pentru dvs. \u0219i pentru mul\u021bi al\u021bi cercet\u0103tori post\u00e2nd o recenzie despre Mind the Graph. Spune\u021bi-ne despre experien\u021ba dvs., doar face\u021bi clic aici<\/a>. <\/p>","protected":false},"excerpt":{"rendered":"

PyScratch este un nou software open-source implementat \u00een Python pentru analiza analizelor de date de migrare, cu o interfa\u021b\u0103 prietenoas\u0103 care permite utilizarea sa de c\u0103tre oamenii de \u0219tiin\u021b\u0103 cu pu\u021bine sau deloc cuno\u0219tin\u021be de programare. Software-ul a fost conceput \u00een cadrul unui parteneriat \u00eentre trei oameni de \u0219tiin\u021b\u0103 brazilieni din laboratorul NanoCell Interactions de la Universitatea din Campinas \u00een 2017. Acesta a fost [...]<\/p>","protected":false},"author":4,"featured_media":12133,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[959,28],"tags":[817,554,964],"yoast_head":"\nA new tool for processing spatial and temporal information<\/title>\n<meta name=\"description\" content=\"Read this blog to know what PyScratch is and how that new tool processes spatial and temporal information from scratch assays.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mindthegraph.com\/blog\/ro\/saggi-pyscratch-spatial-and-temporal-information-from-scratch\/\" \/>\n<meta property=\"og:locale\" content=\"ro_RO\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"A new tool for processing spatial and temporal information\" \/>\n<meta property=\"og:description\" content=\"Read 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He has a Ph.D. and solid scientific background in Psychopharmacology and experience as a Guest Researcher at the Max Planck Institute of Psychiatry (Germany) and Researcher in D'Or Institute for Research and Education (IDOR, Brazil). Fabricio holds over 2500 citations in Google Scholar. He has 10 years of experience in small innovative businesses, with relevant experience in product design and innovation management. 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