{"id":16636,"date":"2022-09-10T18:27:00","date_gmt":"2022-09-10T21:27:00","guid":{"rendered":"https:\/\/mindthegraph.com\/blog\/?p=16636"},"modified":"2023-03-17T14:47:45","modified_gmt":"2023-03-17T17:47:45","slug":"success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids","status":"publish","type":"post","link":"https:\/\/mindthegraph.com\/blog\/pt\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/","title":{"rendered":"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review"},"content":{"rendered":"

A forma\u00e7\u00e3o de esfer\u00f3ides e organ\u00f3ides deficientes pela inibi\u00e7\u00e3o FGFR em c\u00e9lulas CRC derivadas de pacientes \u00e9 muito mais f\u00e1cil de entender usando as ilustra\u00e7\u00f5es do Mind the Graph. <\/p>\n\n\n\n

O esquema criado pelo autor mostra que as bi\u00f3psias cir\u00fargicas foram digeridas enzimaticamente para obter uma suspens\u00e3o de c\u00e9lulas \u00fanicas. As c\u00e9lulas n\u00e3o classificadas foram cultivadas em FGF2 contendo CSC-medium ou com o inibidor FGFR- SU-5402.<\/p>\n\n\n\n

O papel do fator de crescimento fibroblasto 2 (FGF2) na auto-renova\u00e7\u00e3o das c\u00e9lulas-tronco do c\u00e2ncer colorretal tem sido investigado<\/strong><\/h2>\n\n\n\n

Em 'FGF Signalling in the Self-Renewal of Colon Cancer Organoids', J\u00f6rg Otte e colegas (2019) relataram que quando c\u00e9lulas \u00fanicas ing\u00eanuas foram incorporadas no Matrigel e cultivadas no CSC-m\u00e9dio, uma auto-organiza\u00e7\u00e3o mais complexa foi observada em todos os pacientes analisados. <\/p>\n\n\n\n

Genes diretamente associados ao EMT, juntamente com um fen\u00f3tipo mesenquimal e auto-renov\u00e1vel (TGFB3, ID1, ID2 e ID4) foram encontrados superexpressos em organ\u00f3ides. <\/p>\n\n\n\n

Enquanto a inibi\u00e7\u00e3o do FGFR levou a uma sinaliza\u00e7\u00e3o MAPK aprimorada, eles observaram uma diferencia\u00e7\u00e3o induzida, uma perda da maioria dos marcadores de c\u00e9lulas-tronco e um fen\u00f3tipo epitelial. <\/p>\n\n\n\n

As c\u00e9lulas-tronco cancer\u00edgenas s\u00e3o uma subpopula\u00e7\u00e3o de c\u00e9lulas malignas capazes de se auto-renovar e de servir como uma fonte cont\u00ednua de c\u00e9lulas tumorais diferenciadas. Em alguns protocolos de cultura CSC, o fator de crescimento Fibroblast 2 \u00e9 um ingrediente importante para a manuten\u00e7\u00e3o da estaminais. <\/p>\n\n\n\n

No CSC-medium cultive organoides, por outro lado, detectaram indu\u00e7\u00e3o de TGF-\u03b2 e de todos os quatro membros da fam\u00edlia do gene inibidor da diferencia\u00e7\u00e3o.<\/p>\n\n\n\n

9 pacientes consecutivos n\u00e3o selecionados do CRC estiveram envolvidos na pesquisa.<\/strong><\/p>\n\n\n\n

Verifique o artigo original aqui<\/a>.<\/p>\n\n\n\n

Resumo gr\u00e1fico alimentado por Mind the Graph<\/strong><\/h2>\n\n\n\n
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Feito com Mind the Graph<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

Algumas de suas conclus\u00f5es potencialmente refor\u00e7am o que se sabia anteriormente sobre este campo: \"Os genes associados \u00e0 pluripot\u00eancia NANOG, LIN28 e SALL1 foram expressos exclusivamente pelos hESCs. Os GO-terms \"regula\u00e7\u00e3o negativa do caminho de sinaliza\u00e7\u00e3o BMP\" e \"transdu\u00e7\u00e3o de sinal de prote\u00edna SMAD\" foram detectados em nossa cultura hESC\", sugeriu Otte.<\/p>\n\n\n\n

Os autores admitem que \"a an\u00e1lise a termo deste conjunto de genes produziu apenas alguns resultados significativos devido ao baixo n\u00famero de genes comumente regulados\". A maioria destes GO-terms para a condi\u00e7\u00e3o CSC-medium foram associados com transcri\u00e7\u00e3o ativa, prolifera\u00e7\u00e3o celular ou aumento da absor\u00e7\u00e3o de glicose. <\/p>\n\n\n\n

Ap\u00f3s a inibi\u00e7\u00e3o do FGFR, encontramos reguladores do ciclo celular, assim como muitos genes anotados com \"diferencia\u00e7\u00e3o de c\u00e9lulas epiteliais\", \"digest\u00e3o\" ou \"transporte de \u00e1cido biliar e sal biliar\".<\/p>\n\n\n\n

Impacto e desempenho dos trabalhos<\/strong><\/h2>\n\n\n\n

Podemos analisar com Altmetric<\/a> dados de que a pontua\u00e7\u00e3o de aten\u00e7\u00e3o online deste artigo \u00e9 maior do que 68%<\/strong><\/strong> de todos os outros artigos de idade semelhante nos Relat\u00f3rios Cient\u00edficos.<\/strong><\/p>\n\n\n\n

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Fonte Altmetrics<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

Melhore a acessibilidade de seu artigo tamb\u00e9m com o poder dos infogr\u00e1ficos.<\/p>\n\n\n\n

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Experimente Mind the Graph<\/strong><\/a><\/div>\n<\/div>\n\n\n\n
<\/div>","protected":false},"excerpt":{"rendered":"

A forma\u00e7\u00e3o de esfer\u00f3ides e organ\u00f3ides deficientes pela inibi\u00e7\u00e3o FGFR em c\u00e9lulas CRC derivadas de pacientes \u00e9 mais f\u00e1cil de entender com nossas ilustra\u00e7\u00f5es.<\/p>","protected":false},"author":29,"featured_media":20014,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[195,958,957,1],"tags":[96,554,775,963],"yoast_head":"\nFGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog<\/title>\n<meta name=\"description\" content=\"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mindthegraph.com\/blog\/pt\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/\" \/>\n<meta property=\"og:locale\" content=\"pt_BR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - 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