{"id":16636,"date":"2022-09-10T18:27:00","date_gmt":"2022-09-10T21:27:00","guid":{"rendered":"https:\/\/mindthegraph.com\/blog\/?p=16636"},"modified":"2023-03-17T14:47:45","modified_gmt":"2023-03-17T17:47:45","slug":"success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids","status":"publish","type":"post","link":"https:\/\/mindthegraph.com\/blog\/pl\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/","title":{"rendered":"Sygnalizacja FGF w samoodnowie organoid\u00f3w raka okr\u0119\u017cnicy - przegl\u0105d artyku\u0142\u00f3w"},"content":{"rendered":"

Niepe\u0142nosprawne tworzenie sferoid\u00f3w i organoid\u00f3w przez inhibicj\u0119 FGFR w kom\u00f3rkach CRC pochodz\u0105cych od pacjent\u00f3w jest znacznie \u0142atwiejsze do zrozumienia dzi\u0119ki ilustracjom Mind the Graph. <\/p>\n\n\n\n

Schemat stworzony przez autora pokazuje, \u017ce biopsje chirurgiczne by\u0142y enzymatycznie trawione w celu uzyskania zawiesiny pojedynczych kom\u00f3rek. Naiwne, niesortowane kom\u00f3rki hodowano w pod\u0142o\u017cu CSC zawieraj\u0105cym FGF2 lub z inhibitorem FGFR SU-5402.<\/p>\n\n\n\n

Zbadano rol\u0119 czynnika wzrostu fibroblast\u00f3w 2 (FGF2) w samoodnowie kom\u00f3rek macierzystych raka jelita grubego<\/strong><\/h2>\n\n\n\n

W publikacji \"FGF Signalling in the Self-Renewal of Colon Cancer Organoids\" J\u00f6rg Otte i wsp\u00f3\u0142pracownicy (2019) donosz\u0105, \u017ce gdy naiwne pojedyncze kom\u00f3rki osadzono w Matrigelu i hodowano w pod\u0142o\u017cu CSC, u wszystkich analizowanych pacjent\u00f3w zaobserwowano bardziej z\u0142o\u017con\u0105 samoorganizacj\u0119. <\/p>\n\n\n\n

Stwierdzono, \u017ce geny bezpo\u015brednio zwi\u0105zane z EMT wraz z fenotypem mezenchymalnym i samoodnawiaj\u0105cym si\u0119 (TGFB3, ID1, ID2 i ID4) ulega\u0142y nadekspresji w organoidach. <\/p>\n\n\n\n

Podczas gdy inhibicja FGFR prowadzi\u0142a do wzmocnionej sygnalizacji MAPK, zaobserwowano indukowane r\u00f3\u017cnicowanie, utrat\u0119 wi\u0119kszo\u015bci marker\u00f3w kom\u00f3rek macierzystych i fenotyp nab\u0142onkowy. <\/p>\n\n\n\n

Nowotworowe kom\u00f3rki macierzyste s\u0105 subpopulacj\u0105 z\u0142o\u015bliwych kom\u00f3rek zdolnych do samoodnawiania i s\u0142u\u017cenia jako sta\u0142e \u017ar\u00f3d\u0142o dla zr\u00f3\u017cnicowanych kom\u00f3rek nowotworowych. W niekt\u00f3rych protoko\u0142ach hodowli CSC czynnik wzrostu fibroblast\u00f3w 2 jest wa\u017cnym sk\u0142adnikiem utrzymuj\u0105cym macierzysto\u015b\u0107. <\/p>\n\n\n\n

Z drugiej strony, w organoidach hodowanych w CSC wykryto indukcj\u0119 TGF-\u03b2 i wszystkich czterech cz\u0142onk\u00f3w rodziny gen\u00f3w inhibitor\u00f3w r\u00f3\u017cnicowania.<\/p>\n\n\n\n

W badaniu wzi\u0119\u0142o udzia\u0142 9 kolejnych niewyselekcjonowanych pacjent\u00f3w z CRC.<\/strong><\/p>\n\n\n\n

Sprawd\u017a oryginalny artyku\u0142 tutaj<\/a>.<\/p>\n\n\n\n

Abstrakcja graficzna obs\u0142ugiwana przez Mind the Graph<\/strong><\/h2>\n\n\n\n
<\/div>\n\n\n
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Wykonane z Mind the Graph<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

Niekt\u00f3re z ich wniosk\u00f3w potencjalnie wzmacniaj\u0105 to, co wcze\u015bniej by\u0142o wiadomo na ten temat: \"Geny zwi\u0105zane z pluripotencj\u0105 NANOG, LIN28 i SALL1 by\u0142y wyra\u017cane wy\u0142\u0105cznie przez hESC. Terminy GO \"negatywna regulacja szlaku sygna\u0142owego BMP\" i \"transdukcja sygna\u0142u bia\u0142ka SMAD\" zosta\u0142y wykryte w naszej kulturze hESC\" - zasugerowa\u0142 Otte.<\/p>\n\n\n\n

Autorzy przyznaj\u0105, \u017ce \"analiza GO-term tego zestawu gen\u00f3w przynios\u0142a tylko kilka znacz\u0105cych wynik\u00f3w ze wzgl\u0119du na ma\u0142\u0105 liczb\u0119 powszechnie regulowanych gen\u00f3w. Wi\u0119kszo\u015b\u0107 z tych GO-termin\u00f3w dla stanu CSC-\u015bredniego by\u0142a zwi\u0105zana z aktywn\u0105 transkrypcj\u0105, proliferacj\u0105 kom\u00f3rek lub zwi\u0119kszonym wychwytem glukozy. <\/p>\n\n\n\n

Po zahamowaniu FGFR znale\u017ali\u015bmy regulatory cyklu kom\u00f3rkowego, a tak\u017ce wiele gen\u00f3w z adnotacj\u0105 \"r\u00f3\u017cnicowanie kom\u00f3rek nab\u0142onkowych\", \"trawienie\" lub \"transport kwas\u00f3w \u017c\u00f3\u0142ciowych i soli \u017c\u00f3\u0142ciowych\"\".<\/p>\n\n\n\n

Dokumenty Wp\u0142yw i wydajno\u015b\u0107<\/strong><\/h2>\n\n\n\n

Mo\u017cemy analizowa\u0107 za pomoc\u0105 Altmetric<\/a> dane, \u017ce wynik uwagi online tego artyku\u0142u jest wy\u017cszy ni\u017c 68%<\/strong><\/strong> wszystkich innych artyku\u0142\u00f3w w podobnym wieku w czasopi\u015bmie Scientific Reports.<\/strong><\/p>\n\n\n\n

<\/div>\n\n\n
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\u0179r\u00f3d\u0142o Altmetrics<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

Popraw tak\u017ce dost\u0119pno\u015b\u0107 swojego artyku\u0142u dzi\u0119ki infografikom.<\/p>\n\n\n\n

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Wypr\u00f3buj Mind the Graph<\/strong><\/a><\/div>\n<\/div>\n\n\n\n
<\/div>","protected":false},"excerpt":{"rendered":"

Niepe\u0142nosprawne tworzenie sferoid\u00f3w i organoid\u00f3w przez inhibicj\u0119 FGFR w kom\u00f3rkach CRC pochodz\u0105cych od pacjent\u00f3w jest \u0142atwiejsze do zrozumienia dzi\u0119ki naszym ilustracjom.<\/p>","protected":false},"author":29,"featured_media":20014,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[195,958,957,1],"tags":[96,554,775,963],"yoast_head":"\nFGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog<\/title>\n<meta name=\"description\" content=\"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mindthegraph.com\/blog\/pl\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/\" \/>\n<meta property=\"og:locale\" content=\"pl_PL\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - 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