{"id":16636,"date":"2022-09-10T18:27:00","date_gmt":"2022-09-10T21:27:00","guid":{"rendered":"https:\/\/mindthegraph.com\/blog\/?p=16636"},"modified":"2023-03-17T14:47:45","modified_gmt":"2023-03-17T17:47:45","slug":"success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids","status":"publish","type":"post","link":"https:\/\/mindthegraph.com\/blog\/nl\/succes-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/","title":{"rendered":"FGF signalering in de zelfvernieuwing van colonkanker organo\u00efden Paper Review"},"content":{"rendered":"

De uitgeschakelde sfero\u00efde- en organo\u00efdevorming door FGFR-remming in van pati\u00ebnten afkomstige CRC-cellen is veel gemakkelijker te begrijpen aan de hand van de illustraties van Mind the Graph. <\/p>\n\n\n\n

Volgens het door de auteur opgestelde schema werden de chirurgische biopten enzymatisch verteerd om een suspensie van losse cellen te verkrijgen. De na\u00efeve ongesorteerde cellen werden gekweekt in FGF2-bevattend CSC-medium of met de FGFR-remmer SU-5402.<\/p>\n\n\n\n

De rol van fibroblast groeifactor 2 (FGF2) in de zelfvernieuwing van colorectale kankerstamcellen is onderzocht<\/strong><\/h2>\n\n\n\n

In 'FGF Signalling in the Self-Renewal of Colon Cancer Organoids', meldden J\u00f6rg Otte en collega's (2019) dat wanneer na\u00efeve enkelvoudige cellen werden ingebed in Matrigel en gekweekt in het CSC-medium, een meer complexe zelforganisatie werd waargenomen in alle geanalyseerde pati\u00ebnten. <\/p>\n\n\n\n

Genen die rechtstreeks verband houden met EMT in combinatie met een mesenchymaal en zelfvernieuwend fenotype (TGFB3, ID1, ID2 en ID4) bleken overge\u00ebxpresseerd te zijn in organo\u00efden. <\/p>\n\n\n\n

Terwijl FGFR-remming leidde tot een versterkte MAPK-signalering, constateerden zij ge\u00efnduceerde differentiatie, een verlies van de meeste stamcelmarkers en een epitheliaal fenotype. <\/p>\n\n\n\n

Kankerstamcellen zijn een subpopulatie van kwaadaardige cellen die zichzelf kunnen vernieuwen en kunnen dienen als een permanente bron voor gedifferentieerde tumorcellen. In sommige protocollen van CSC-kweek is Fibroblastgroeifactor 2 een belangrijk ingredi\u00ebnt voor het behoud van stamcellen. <\/p>\n\n\n\n

In CSC-medium gekweekte organo\u00efden ontdekten zij daarentegen inductie van TGF-\u03b2 en van alle vier de leden van de familie van genen die de differentiatie remmen.<\/p>\n\n\n\n

9 opeenvolgende niet-geselecteerde CRC-pati\u00ebnten werden bij het onderzoek betrokken.<\/strong><\/p>\n\n\n\n

Controleer de origineel artikel hier<\/a>.<\/p>\n\n\n\n

Grafisch Abstract aangedreven door Mind the Graph<\/strong><\/h2>\n\n\n\n
<\/div>\n\n\n
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Gemaakt met Mind the Graph<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

Sommige van hun conclusies versterken mogelijk wat eerder over dit gebied bekend was: \"De pluripotentie-geassocieerde genen NANOG, LIN28 en SALL1 werden uitsluitend door hESC's tot expressie gebracht. De GO-termen \"negatieve regulering van BMP-signaalroute\" en \"SMAD-eiwitsignaaltransductie\" werden gedetecteerd in onze hESC-kweek,\" stelde Otte.<\/p>\n\n\n\n

De auteurs geven toe dat \"GO-term-analyse van deze genenset slechts enkele significante resultaten opleverde vanwege het geringe aantal gemeenschappelijk gereguleerde genen. De meeste van deze GO-termen voor de CSC-medium conditie werden geassocieerd met actieve transcriptie, celproliferatie of verhoogde glucose-opname. <\/p>\n\n\n\n

Na FGFR-remming vonden we celcyclusregulatoren en veel genen die geannoteerd zijn met \"epitheliale celdifferentiatie\", \"spijsvertering\" of \"galzuur- en galzouttransport\".\"<\/p>\n\n\n\n

Documenten Impact en prestaties<\/strong><\/h2>\n\n\n\n

We kunnen analyseren met Altmetric<\/a> gegevens dat de online aandachtsscore van deze krant hoger is... dan 68%<\/strong><\/strong> van alle andere artikelen van dezelfde leeftijd in Scientific Reports.<\/strong><\/p>\n\n\n\n

<\/div>\n\n\n
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Bron Altmetrics<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

Verbeter ook de toegankelijkheid van uw artikel met de kracht van infographics.<\/p>\n\n\n\n

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Probeer Mind the Graph<\/strong><\/a><\/div>\n<\/div>\n\n\n\n
<\/div>","protected":false},"excerpt":{"rendered":"

De uitgeschakelde sfero\u00efde- en organo\u00efdevorming door FGFR-remming in van pati\u00ebnten afkomstige CRC-cellen is gemakkelijker te begrijpen met onze illustraties.<\/p>","protected":false},"author":29,"featured_media":20014,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[195,958,957,1],"tags":[96,554,775,963],"yoast_head":"\nFGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog<\/title>\n<meta name=\"description\" content=\"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mindthegraph.com\/blog\/nl\/succes-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/\" \/>\n<meta property=\"og:locale\" content=\"nl_NL\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - 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