{"id":16636,"date":"2022-09-10T18:27:00","date_gmt":"2022-09-10T21:27:00","guid":{"rendered":"https:\/\/mindthegraph.com\/blog\/?p=16636"},"modified":"2023-03-17T14:47:45","modified_gmt":"2023-03-17T17:47:45","slug":"success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids","status":"publish","type":"post","link":"https:\/\/mindthegraph.com\/blog\/nb\/suksess-case-fgf-signalering-i-selvfornyelsen-av-tarmkreft-organoider\/","title":{"rendered":"FGF-signalering i selvfornyelse av organoider for tykktarmskreft Paper Review"},"content":{"rendered":"

The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells is much easier to understand using Mind the Graph\u2019s illustrations. <\/p>\n\n\n\n

Skjemaet som forfatteren har laget, viser at de kirurgiske biopsiene ble ford\u00f8yd enzymatisk for \u00e5 f\u00e5 en suspensjon av enkeltceller. De naive, usorterte cellene ble dyrket i FGF2-holdig CSC-medium eller med FGFR-hemmeren SU-5402.<\/p>\n\n\n\n

Man har unders\u00f8kt hvilken rolle fibroblastvekstfaktor 2 (FGF2) spiller i selvfornyelsen av stamceller fra kolorektal kreft.<\/strong><\/h2>\n\n\n\n

In \u2018FGF Signalling in the Self-Renewal of Colon Cancer Organoids\u2019, J\u00f6rg Otte and colleagues (2019) reported that when na\u00efve single cells were embedded in Matrigel and cultured in the CSC-medium, a more complex self-organization was observed in all patients analysed. <\/p>\n\n\n\n

Gener som er direkte assosiert med EMT sammen med en mesenkymal og selvfornyende fenotype (TGFB3, ID1, ID2 og ID4) ble funnet \u00e5 v\u00e6re overuttrykt i organoider. <\/p>\n\n\n\n

Mens FGFR-hemming f\u00f8rte til \u00f8kt MAPK-signalering, observerte de indusert differensiering, tap av de fleste stamcellemark\u00f8rer og en epitelial fenotype. <\/p>\n\n\n\n

Kreftstamceller er en subpopulasjon av ondartede celler som er i stand til \u00e5 fornye seg selv og fungere som en kontinuerlig kilde for differensierte tumorceller. I noen protokoller for CSC-dyrking er fibroblastvekstfaktor 2 en viktig ingrediens for \u00e5 opprettholde stamcellene. <\/p>\n\n\n\n

I organoider dyrket i CSC-medium p\u00e5viste de derimot induksjon av TGF-\u03b2 og av alle de fire medlemmene av genfamilien inhibitor-of-differentiation.<\/p>\n\n\n\n

9 konsekutive, uselekterte CRC-pasienter deltok i unders\u00f8kelsen.<\/strong><\/p>\n\n\n\n

Kontroller originalartikkel her<\/a>.<\/p>\n\n\n\n

Grafisk sammendrag drevet av Mind the Graph<\/strong><\/h2>\n\n\n\n
<\/div>\n\n\n
\n
\"\"<\/a>
Laget med Mind the Graph<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

Some of their conclusions potentially strengthen what was previously known about this field: \u201cThe pluripotency-associated genes NANOG, LIN28 and SALL1 were exclusively expressed by hESCs. The GO-terms \u201cnegative regulation of BMP signaling pathway\u201d and \u201cSMAD protein signal transduction\u201d were detected in our hESC culture,\u201d Otte suggested.<\/p>\n\n\n\n

Forfatterne innr\u00f8mmer at \"GO-term-analyse av dette gensettet ga bare noen f\u00e5 signifikante resultater p\u00e5 grunn av det lave antallet vanlig regulerte gener. De fleste av disse GO-termene for CSC-medium-tilstanden var assosiert med aktiv transkripsjon, celleproliferasjon eller \u00f8kt glukoseopptak. <\/p>\n\n\n\n

Etter FGFR-inhibering fant vi cellesyklusregulatorer samt mange gener som er knyttet til \"epitelcelledifferensiering\", \"ford\u00f8yelse\" eller \"gallesyre- og gallesalttransport\".\"<\/p>\n\n\n\n

Avisenes innvirkning og resultater<\/strong><\/h2>\n\n\n\n

Vi kan analysere med Altmetric<\/a> data at denne avisens oppmerksomhetsscore p\u00e5 nett er h\u00f8yere enn 68%<\/strong><\/strong> av alle andre artikler av tilsvarende alder i Scientific Reports.<\/strong><\/p>\n\n\n\n

<\/div>\n\n\n
\n
\"\"<\/a>
Kilde Altmetrikk<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

Forbedre artikkelens tilgjengelighet ved hjelp av infografikk.<\/p>\n\n\n\n

\n
Pr\u00f8v Mind the Graph<\/strong><\/a><\/div>\n<\/div>\n\n\n\n
<\/div>","protected":false},"excerpt":{"rendered":"

V\u00e5re illustrasjoner gj\u00f8r det lettere \u00e5 forst\u00e5 hvordan FGFR-hemming i pasientavledede CRC-celler f\u00f8rer til dannelse av sf\u00e6roider og organoider.<\/p>","protected":false},"author":29,"featured_media":20014,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[195,958,957,1],"tags":[96,554,775,963],"yoast_head":"\nFGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog<\/title>\n<meta name=\"description\" content=\"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mindthegraph.com\/blog\/nb\/suksess-case-fgf-signalering-i-selvfornyelsen-av-tarmkreft-organoider\/\" \/>\n<meta property=\"og:locale\" content=\"nb_NO\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog\" \/>\n<meta property=\"og:description\" content=\"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/mindthegraph.com\/blog\/nb\/suksess-case-fgf-signalering-i-selvfornyelsen-av-tarmkreft-organoider\/\" \/>\n<meta property=\"og:site_name\" content=\"Mind the Graph Blog\" \/>\n<meta property=\"article:published_time\" content=\"2022-09-10T21:27:00+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2023-03-17T17:47:45+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2022\/09\/blog_4.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1125\" \/>\n\t<meta property=\"og:image:height\" content=\"614\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Artur Zignani\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:image\" content=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2022\/09\/blog_4.jpg\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Artur Zignani\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"3 minutes\" \/>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog","description":"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/mindthegraph.com\/blog\/nb\/suksess-case-fgf-signalering-i-selvfornyelsen-av-tarmkreft-organoider\/","og_locale":"nb_NO","og_type":"article","og_title":"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog","og_description":"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.","og_url":"https:\/\/mindthegraph.com\/blog\/nb\/suksess-case-fgf-signalering-i-selvfornyelsen-av-tarmkreft-organoider\/","og_site_name":"Mind the Graph Blog","article_published_time":"2022-09-10T21:27:00+00:00","article_modified_time":"2023-03-17T17:47:45+00:00","og_image":[{"width":1125,"height":614,"url":"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2022\/09\/blog_4.jpg","type":"image\/jpeg"}],"author":"Artur Zignani","twitter_card":"summary_large_image","twitter_image":"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2022\/09\/blog_4.jpg","twitter_misc":{"Written by":"Artur Zignani","Est. reading time":"3 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/mindthegraph.com\/blog\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/","url":"https:\/\/mindthegraph.com\/blog\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/","name":"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog","isPartOf":{"@id":"https:\/\/mindthegraph.com\/blog\/#website"},"datePublished":"2022-09-10T21:27:00+00:00","dateModified":"2023-03-17T17:47:45+00:00","author":{"@id":"https:\/\/mindthegraph.com\/blog\/#\/schema\/person\/b44995c1d77b52a63b6d11262b740b02"},"description":"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.","breadcrumb":{"@id":"https:\/\/mindthegraph.com\/blog\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/#breadcrumb"},"inLanguage":"nb-NO","potentialAction":[{"@type":"ReadAction","target":["https:\/\/mindthegraph.com\/blog\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/mindthegraph.com\/blog\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/mindthegraph.com\/blog\/"},{"@type":"ListItem","position":2,"name":"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review"}]},{"@type":"WebSite","@id":"https:\/\/mindthegraph.com\/blog\/#website","url":"https:\/\/mindthegraph.com\/blog\/","name":"Mind the Graph Blog","description":"Your science can be beautiful!","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/mindthegraph.com\/blog\/?s={search_term_string}"},"query-input":"required name=search_term_string"}],"inLanguage":"nb-NO"},{"@type":"Person","@id":"https:\/\/mindthegraph.com\/blog\/#\/schema\/person\/b44995c1d77b52a63b6d11262b740b02","name":"Artur Zignani","image":{"@type":"ImageObject","inLanguage":"nb-NO","@id":"https:\/\/mindthegraph.com\/blog\/#\/schema\/person\/image\/","url":"https:\/\/secure.gravatar.com\/avatar\/a211ec97011fe24f7a8e63a7ca799342?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/a211ec97011fe24f7a8e63a7ca799342?s=96&d=mm&r=g","caption":"Artur Zignani"},"url":"https:\/\/mindthegraph.com\/blog\/nb\/author\/artur\/"}]}},"_links":{"self":[{"href":"https:\/\/mindthegraph.com\/blog\/nb\/wp-json\/wp\/v2\/posts\/16636"}],"collection":[{"href":"https:\/\/mindthegraph.com\/blog\/nb\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/mindthegraph.com\/blog\/nb\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/mindthegraph.com\/blog\/nb\/wp-json\/wp\/v2\/users\/29"}],"replies":[{"embeddable":true,"href":"https:\/\/mindthegraph.com\/blog\/nb\/wp-json\/wp\/v2\/comments?post=16636"}],"version-history":[{"count":5,"href":"https:\/\/mindthegraph.com\/blog\/nb\/wp-json\/wp\/v2\/posts\/16636\/revisions"}],"predecessor-version":[{"id":27203,"href":"https:\/\/mindthegraph.com\/blog\/nb\/wp-json\/wp\/v2\/posts\/16636\/revisions\/27203"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/mindthegraph.com\/blog\/nb\/wp-json\/wp\/v2\/media\/20014"}],"wp:attachment":[{"href":"https:\/\/mindthegraph.com\/blog\/nb\/wp-json\/wp\/v2\/media?parent=16636"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/mindthegraph.com\/blog\/nb\/wp-json\/wp\/v2\/categories?post=16636"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/mindthegraph.com\/blog\/nb\/wp-json\/wp\/v2\/tags?post=16636"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}