{"id":16636,"date":"2022-09-10T18:27:00","date_gmt":"2022-09-10T21:27:00","guid":{"rendered":"https:\/\/mindthegraph.com\/blog\/?p=16636"},"modified":"2023-03-17T14:47:45","modified_gmt":"2023-03-17T17:47:45","slug":"success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids","status":"publish","type":"post","link":"https:\/\/mindthegraph.com\/blog\/lv\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/","title":{"rendered":"FGF signaliz\u0101cija resn\u0101s zarnas v\u0113\u017ea organo\u012bdu pa\u0161atjauno\u0161an\u0101s proces\u0101 Pap\u012bra apskats"},"content":{"rendered":"

Inval\u012bdu sfero\u012bdu un organo\u012bdu veido\u0161anos ar FGFR inhib\u012bciju no pacientiem ieg\u016bt\u0101s CRC \u0161\u016bn\u0101s ir daudz viegl\u0101k saprast, izmantojot Mind the Graph ilustr\u0101cijas. <\/p>\n\n\n\n

Autora izveidot\u0101 sh\u0113ma par\u0101da, ka \u0137irur\u0123isk\u0101s biopsijas tika fermentat\u012bvi sa\u0161\u0137eltas, lai ieg\u016btu atsevi\u0161\u0137u \u0161\u016bnu suspensiju. Naiv\u0101s ne\u0161\u0137irot\u0101s \u0161\u016bnas kultiv\u0113ja FGF2 saturo\u0161\u0101 CSC vid\u0113 vai ar FGFR inhibitoru SU-5402.<\/p>\n\n\n\n

Izp\u0113t\u012bta 2. fibroblastu aug\u0161anas faktora (FGF2) loma kolorekt\u0101l\u0101 v\u0113\u017ea cilmes \u0161\u016bnu pa\u0161atjauno\u0161an\u0101s proces\u0101.<\/strong><\/h2>\n\n\n\n

Darb\u0101 \"FGF signaliz\u0101cija resn\u0101s zarnas v\u0113\u017ea organo\u012bdu pa\u0161atjauno\u0161an\u0101s proces\u0101\" J\u00f6rg Otte un kol\u0113\u0123i (2019) zi\u0146oja, ka, ja naiv\u0101s atsevi\u0161\u0137\u0101s \u0161\u016bnas tika iestr\u0101d\u0101tas Matrigel\u0101 un kultiv\u0113tas CSC vid\u0113, visiem analiz\u0113tajiem pacientiem tika nov\u0113rota sare\u017e\u0123\u012bt\u0101ka pa\u0161organiz\u0101cija. <\/p>\n\n\n\n

Tika konstat\u0113ts, ka g\u0113ni, kas tie\u0161i saist\u012bti ar EMT un mezenh\u012bma un pa\u0161atjauno\u0161an\u0101s fenotipu (TGFB3, ID1, ID2 un ID4), organo\u012bdos ir p\u0101rm\u0113r\u012bgi ekspres\u0113ti. <\/p>\n\n\n\n

Lai gan FGFR inhib\u012bcija izrais\u012bja pastiprin\u0101tu MAPK signaliz\u0101ciju, tika nov\u0113rota induc\u0113ta diferenci\u0101cija, vairuma cilmes \u0161\u016bnu mar\u0137ieru zudums un epit\u0113lija fenotips. <\/p>\n\n\n\n

V\u0113\u017ea cilmes \u0161\u016bnas ir \u013caundab\u012bgo \u0161\u016bnu subpopul\u0101cija, kas sp\u0113j pati atjaunoties un kalpot par past\u0101v\u012bgu diferenc\u0113tu audz\u0113ja \u0161\u016bnu avotu. Da\u017eos CSC kult\u016bras protokolos fibroblastu aug\u0161anas faktors 2 ir svar\u012bga sast\u0101vda\u013ca cilmes saglab\u0101\u0161anai. <\/p>\n\n\n\n

No otras puses, CSC vid\u0113 kultiv\u0113tos organo\u012bdos tika konstat\u0113ta TGF-\u03b2 un visu \u010detru diferenci\u0101cijas inhibitoru g\u0113nu saimes locek\u013cu indukcija.<\/p>\n\n\n\n

P\u0113t\u012bjum\u0101 tika iesaist\u012bti 9 p\u0113c k\u0101rtas atlas\u012bti CRC pacienti.<\/strong><\/p>\n\n\n\n

P\u0101rbaudiet ori\u0123in\u0101lais raksts \u0161eit<\/a>.<\/p>\n\n\n\n

Grafiskais abstrakts, ko nodro\u0161ina Mind the Graph<\/strong><\/h2>\n\n\n\n
<\/div>\n\n\n
\n
\"\"<\/a>
Izgatavots ar Mind the Graph<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

Da\u017ei no secin\u0101jumiem, iesp\u0113jams, nostiprina iepriek\u0161 zin\u0101m\u0101s atzi\u0146as par \u0161o jomu: \"Ar pluripotenci saist\u012btie g\u0113ni NANOG, LIN28 un SALL1 tika izteikti tikai hESCs. M\u016bsu hESC kult\u016br\u0101 tika konstat\u0113ti GO termini \"BMP sign\u0101lu ce\u013ca negat\u012bva regul\u0101cija\" un \"SMAD prote\u012bnu sign\u0101lu transdukcija\",\" ierosin\u0101ja Otte.<\/p>\n\n\n\n

Autori atz\u012bst, ka \"\u0161\u012b g\u0113nu kopuma GO-term anal\u012bze deva tikai da\u017eus noz\u012bm\u012bgus rezult\u0101tus, jo parasti regul\u0113to g\u0113nu skaits ir neliels. Liel\u0101k\u0101 da\u013ca no \u0161iem GO-termiem CSC-vid\u0113j\u0101 st\u0101vokl\u012b bija saist\u012bti ar akt\u012bvu transkripciju, \u0161\u016bnu prolifer\u0101ciju vai palielin\u0101tu glikozes uz\u0146em\u0161anu. <\/p>\n\n\n\n

P\u0113c FGFR inhib\u012bcijas m\u0113s atrad\u0101m \u0161\u016bnu cikla regulatorus, k\u0101 ar\u012b daudzus g\u0113nus, kas anot\u0113ti ar \"epit\u0113lija \u0161\u016bnu diferenci\u0101ciju\", \"gremo\u0161anu\" vai \"\u017eultssk\u0101bju un \u017eults s\u0101\u013cu transportu\".\"\"\"<\/p>\n\n\n\n

Dokumenti Ietekme un veiktsp\u0113ja<\/strong><\/h2>\n\n\n\n

M\u0113s varam analiz\u0113t ar Altmetric<\/a> dati, ka \u0161\u012b dokumenta tie\u0161saistes uzman\u012bbas r\u0101d\u012bt\u0101js ir augst\u0101ks. nek\u0101 68%<\/strong><\/strong> no visiem citiem l\u012bdz\u012bga vecuma rakstiem izdevum\u0101 Scientific Reports.<\/strong><\/p>\n\n\n\n

<\/div>\n\n\n
\n
\"\"<\/a>
Avots: Altmetrika<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

Uzlabojiet raksta pieejam\u012bbu, izmantojot ar\u012b infografikas iesp\u0113jas.<\/p>\n\n\n\n

\n
Izm\u0113\u0123iniet Mind the Graph<\/strong><\/a><\/div>\n<\/div>\n\n\n\n
<\/div>","protected":false},"excerpt":{"rendered":"

Inval\u012bdu sfero\u012bdu un organo\u012bdu veido\u0161anos ar FGFR inhib\u012bciju no pacientiem ieg\u016bt\u0101s CRC \u0161\u016bn\u0101s ir viegl\u0101k saprast, izmantojot m\u016bsu ilustr\u0101cijas.<\/p>","protected":false},"author":29,"featured_media":20014,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[195,958,957,1],"tags":[96,554,775,963],"yoast_head":"\nFGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog<\/title>\n<meta name=\"description\" content=\"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mindthegraph.com\/blog\/lv\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/\" \/>\n<meta property=\"og:locale\" content=\"lv_LV\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - 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