{"id":16636,"date":"2022-09-10T18:27:00","date_gmt":"2022-09-10T21:27:00","guid":{"rendered":"https:\/\/mindthegraph.com\/blog\/?p=16636"},"modified":"2023-03-17T14:47:45","modified_gmt":"2023-03-17T17:47:45","slug":"success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids","status":"publish","type":"post","link":"https:\/\/mindthegraph.com\/blog\/lt\/sekmes-atvejis-fgf-signalizavimas-storosios-zarnos-vezio-organoidu-saves-atnaujinime\/","title":{"rendered":"FGF signalai storosios \u017earnos v\u0117\u017eio organoid\u0173 savaiminio atsinaujinimo procese Straipsni\u0173 ap\u017evalga"},"content":{"rendered":"

Ne\u012fgali\u0173j\u0173 sferoid\u0173 ir organoid\u0173 formavim\u0105si slopinant FGFR pacient\u0173 CRC l\u0105stel\u0117se daug lengviau suprasti naudojant Mind the Graph iliustracijas. <\/p>\n\n\n\n

Autoriaus sukurtoje schemoje matyti, kad chirurgin\u0117s biopsijos buvo fermenti\u0161kai skaidomos, kad b\u016bt\u0173 gauta pavieni\u0173 l\u0105steli\u0173 suspensija. Naivios ner\u016b\u0161iuotos l\u0105stel\u0117s buvo auginamos FGF2 turin\u010dioje CSC terp\u0117je arba su FGFR inhibitoriumi SU-5402.<\/p>\n\n\n\n

I\u0161tirtas 2 fibroblast\u0173 augimo faktoriaus (FGF2) vaidmuo kolorektalinio v\u0117\u017eio kamienini\u0173 l\u0105steli\u0173 savitam atsinaujinimui<\/strong><\/h2>\n\n\n\n

Straipsnyje \"FGF signalai storosios \u017earnos v\u0117\u017eio organoid\u0173 sav\u0119s atsinaujinime\" J\u00f6rg Otte su kolegomis (2019) prane\u0161\u0117, kad, kai naivios pavien\u0117s l\u0105stel\u0117s buvo \u012fterptos \u012f Matrigel\u012f ir kultivuojamos CSC terp\u0117je, visiems tiriamiems pacientams buvo pasteb\u0117ta sud\u0117tingesn\u0117 saviorganizacija. <\/p>\n\n\n\n

Buvo nustatyta, kad organoiduose yra per daug i\u0161reik\u0161ti genai, tiesiogiai susij\u0119 su EMT ir mezenchiminiu bei savaime atsinaujinan\u010diu fenotipu (TGFB3, ID1, ID2 ir ID4). <\/p>\n\n\n\n

Nors slopinant FGFR suaktyv\u0117jo MAPK signalizacija, jie pasteb\u0117jo indukuot\u0105 diferenciacij\u0105, daugumos kamienini\u0173 l\u0105steli\u0173 \u017eymen\u0173 praradim\u0105 ir epitelio fenotip\u0105. <\/p>\n\n\n\n

V\u0117\u017eio kamienin\u0117s l\u0105stel\u0117s - tai piktybini\u0173 l\u0105steli\u0173 subpopuliacija, galinti savaime atsinaujinti ir b\u016bti nuolatiniu diferencijuot\u0173 naviko l\u0105steli\u0173 \u0161altiniu. Kai kuriuose CSC kultivavimo protokoluose Fibroblast\u0173 augimo faktorius 2 yra svarbi sudedamoji kamieni\u0161kumo palaikymo dalis. <\/p>\n\n\n\n

Kita vertus, CSC terp\u0117je kultivuotuose organoiduose jie aptiko TGF-\u03b2 ir vis\u0173 keturi\u0173 diferenciacijos inhibitori\u0173 \u0161eimos gen\u0173 indukcij\u0105.<\/p>\n\n\n\n

Tyrime dalyvavo 9 i\u0161 eil\u0117s neatrinkti KRK pacientai.<\/strong><\/p>\n\n\n\n

Patikrinkite originalus straipsnis \u010dia<\/a>.<\/p>\n\n\n\n

Grafinis abstraktas, paremtas Mind the Graph<\/strong><\/h2>\n\n\n\n
<\/div>\n\n\n
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\"\"<\/a>
Pagaminta i\u0161 Mind the Graph<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

Kai kurios j\u0173 i\u0161vados gali sustiprinti tai, kas anks\u010diau buvo \u017einoma apie \u0161i\u0105 srit\u012f: \"Su pluripotencija susij\u0119 genai NANOG, LIN28 ir SALL1 buvo i\u0161skirtinai i\u0161reik\u0161ti hESC. M\u016bs\u0173 hESC kult\u016broje buvo aptikti GO terminai \"neigiamas BMP signalinio kelio reguliavimas\" ir \"SMAD baltymo signalo perdavimas\", - si\u016bl\u0117 Otte.<\/p>\n\n\n\n

Autoriai pripa\u017e\u012fsta, kad \"\u0161io gen\u0173 rinkinio GO termin\u0173 analiz\u0117 dav\u0117 tik kelet\u0105 reik\u0161ming\u0173 rezultat\u0173 d\u0117l ma\u017eo bendrai reguliuojam\u0173 gen\u0173 skai\u010diaus. Dauguma \u0161i\u0173 GO-termin\u0173, skirt\u0173 CSC-vidutinei b\u016bklei, buvo susij\u0119 su aktyvia transkripcija, l\u0105steli\u0173 proliferacija arba padid\u0117jusiu gliukoz\u0117s pasisavinimu. <\/p>\n\n\n\n

Po FGFR slopinimo radome l\u0105stel\u0117s ciklo reguliatori\u0173 ir daug gen\u0173, priskiriam\u0173 epitelio l\u0105steli\u0173 diferenciacijos, vir\u0161kinimo arba tul\u017eies r\u016bg\u0161\u010di\u0173 ir tul\u017eies drusk\u0173 perna\u0161os sritims.\"\"<\/p>\n\n\n\n

Dokument\u0173 poveikis ir na\u0161umas<\/strong><\/h2>\n\n\n\n

Galime analizuoti su Altmetric<\/a> duomen\u0173, kad \u0161io dokumento d\u0117mesio internete balas yra didesnis nei 68%<\/strong><\/strong> vis\u0173 kit\u0173 pana\u0161aus am\u017eiaus straipsni\u0173 \u017eurnale \"Scientific Reports\".<\/strong><\/p>\n\n\n\n

<\/div>\n\n\n
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\"\"<\/a>
\u0160altinis Altmetrika<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

Pagerinkite savo straipsnio prieinamum\u0105 naudodami infografik\u0105.<\/p>\n\n\n\n

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I\u0161bandykite Mind the Graph<\/strong><\/a><\/div>\n<\/div>\n\n\n\n
<\/div>","protected":false},"excerpt":{"rendered":"

Ne\u012fgali\u0173j\u0173 sferoid\u0173 ir organoid\u0173 formavim\u0105si slopinant FGFR i\u0161 pacient\u0173 gautose CRC l\u0105stel\u0117se lengviau suprasti i\u0161 m\u016bs\u0173 iliustracij\u0173.<\/p>","protected":false},"author":29,"featured_media":20014,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[195,958,957,1],"tags":[96,554,775,963],"yoast_head":"\nFGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog<\/title>\n<meta name=\"description\" content=\"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mindthegraph.com\/blog\/lt\/sekmes-atvejis-fgf-signalizavimas-storosios-zarnos-vezio-organoidu-saves-atnaujinime\/\" \/>\n<meta property=\"og:locale\" content=\"lt_LT\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog\" \/>\n<meta property=\"og:description\" content=\"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/mindthegraph.com\/blog\/lt\/sekmes-atvejis-fgf-signalizavimas-storosios-zarnos-vezio-organoidu-saves-atnaujinime\/\" \/>\n<meta property=\"og:site_name\" content=\"Mind the Graph Blog\" \/>\n<meta property=\"article:published_time\" content=\"2022-09-10T21:27:00+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2023-03-17T17:47:45+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2022\/09\/blog_4.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1125\" \/>\n\t<meta property=\"og:image:height\" content=\"614\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Artur Zignani\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:image\" content=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2022\/09\/blog_4.jpg\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Artur Zignani\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"3 minutes\" \/>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - 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