{"id":12979,"date":"2021-06-08T20:10:05","date_gmt":"2021-06-08T23:10:05","guid":{"rendered":"https:\/\/mindthegraph.com\/blog\/?p=12979"},"modified":"2023-01-05T14:22:32","modified_gmt":"2023-01-05T17:22:32","slug":"microscopy-how-scientists-use-fluorescence-to-see-inside-cells","status":"publish","type":"post","link":"https:\/\/mindthegraph.com\/blog\/ko\/%ed%98%84%eb%af%b8%ea%b2%bd-%ea%b3%bc%ed%95%99%ec%9e%90%eb%93%a4%ec%9d%b4-%ed%98%95%ea%b4%91%ec%9d%84-%ec%82%ac%ec%9a%a9%ed%95%98%ec%97%ac-%ec%84%b8%ed%8f%ac-%eb%82%b4%eb%b6%80%eb%a5%bc-%eb%b3%b4\/","title":{"rendered":"\ud604\ubbf8\uacbd: \uacfc\ud559\uc790\ub4e4\uc774 \ud615\uad11\uc744 \uc0ac\uc6a9\ud558\uc5ec \uc138\ud3ec \ub0b4\ubd80\ub97c \ubcf4\ub294 \ubc29\ubc95"},"content":{"rendered":"

\uacfc\ud559\uc790\uc758 \uc77c\uacfc\uc5d0\uc11c \uac00\uc7a5 \ud070 \ubd80\ubd84\uc740 \uc2e4\ud5d8\uc744 \uc124\uacc4\ud558\uace0 \uc218\ud589\ud558\ub294 \uac83\uc785\ub2c8\ub2e4.<\/p>\n\n\n\n

\uc2e4\ud5d8\uc2e4 \uae30\uc220\uc758 \uc870\ud569\uc740 \uacfc\ud559\uc790\uac00 \uc81c\uc548\ud55c \ub300\ubd80\ubd84\uc758 \uc9c8\ubb38\uc5d0 \ub300\ud55c \ub2f5\uc744 \uc81c\uacf5\ud558\uba70, \uc0c8\ub85c\uc6b4 \ubc29\ubc95\uc744 \uc81c\uc548\ud558\ub294 \uc6cc\ud06c\ud50c\ub85c\ub294 \uacfc\ud559\uc790\uc758 \ubc30\uacbd\uacfc \uacbd\ud5d8\uc5d0 \ub530\ub77c \ub2ec\ub77c\uc9d1\ub2c8\ub2e4.<\/p>\n\n\n\n

\uc0dd\ubb3c\ud559\uc790\uc5d0\uac8c \uc138\ud3ec \uc774\ubbf8\uc9c0\ub294 \uc5f0\uad6c \uc911\uc778 \uacfc\uc815\uacfc \uba54\ucee4\ub2c8\uc998\uc5d0 \ub300\ud574 \ub9ce\uc740 \uac83\uc744 \uc54c\ub824\uc904 \uc218 \uc788\uc2b5\ub2c8\ub2e4.<\/p>\n\n\n\n

\uad11\ud559 \ud604\ubbf8\uacbd<\/strong> \ub294 \uc0dd\ubb3c \uacfc\ud559 \ubd84\uc57c\uc5d0\uc11c \ub9e4\uc6b0 \ub110\ub9ac \uc0ac\uc6a9\ub418\ub294 \uae30\uc220\uc785\ub2c8\ub2e4.<\/p>\n\n\n\n

\uacfc\ud559\uc790\ub4e4\uc740 \uc5fc\ub8cc, \ud56d\uccb4, \ud615\uad11 \ud504\ub85c\ube0c\ub97c \uc0ac\uc6a9\ud558\uc5ec \ub108\ubb34 \uc791\uc544\uc11c \ubcfc \uc218\ub3c4 \uc5c6\uace0 \uc774\ud574\ud560 \uc218\ub3c4 \uc5c6\uc5c8\ub358 \uc138\ud3ec\uc758 \uc774\ubbf8\uc9c0\ub97c \ud604\ubbf8\uacbd\uc73c\ub85c \ubcfc \uc218 \uc788\uc2b5\ub2c8\ub2e4.<\/p>\n\n\n\n

\ud615\uad11 \ud604\ubbf8\uacbd<\/strong> \ud615\uad11\ub4f1 \uc0ac\uc6a9 1930\ub144\uc5d0 \uac00\ub2a5\ud574\uc84c\uc2b5\ub2c8\ub2e4.1<\/sup><\/a>\uc624\ub298\ub0a0\uc5d0\ub294 \uc138\ud3ec\uc640 \uc870\uc9c1 \ub0b4\uc758 \ub2e8\ubc31\uc9c8, \uc18c\uae30\uad00 \ubc0f \uad6c\uc870\ub97c \uc5fc\uc0c9\ud558\uae30 \uc704\ud574 \ub2e4\uc591\ud55c \ud615\uad11 \uc5fc\ub8cc \uc870\ud569\uc744 \uc0ac\uc6a9\ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4.<\/p>\n\n\n\n

\ud615\uad11\ub4f1(\ub610\ub294 \ud615\uad11\uccb4)<\/strong> \ub294 \ud2b9\uc815 \ud30c\uc7a5\uc758 \ube5b\uc73c\ub85c \uc5ec\uae30\ub418\uba74 \ud604\ubbf8\uacbd\uc758 \ub80c\uc988\uc5d0 \ud3ec\ucc29\ub418\uc5b4 \uc2e4\uc81c \uc774\ubbf8\uc9c0\ub85c \ubcc0\ud658\ub418\ub294 \uc815\ud574\uc9c4 \ud30c\uc7a5\uc758 \ube5b\uc744 \ubc29\ucd9c\ud558\ub294 \ubd84\uc790\uc785\ub2c8\ub2e4.<\/p>\n\n\n\n

\ud615\uad11, \ub80c\uc988, \uce74\uba54\ub77c\uc758 \uc870\ud569\uc744 \ud1b5\ud574 \uc138\ud3ec \ub0b4\ubd80\uc758 \ud504\ub85c\uc138\uc2a4\ub97c \ub2e4\uc591\ud55c \uad00\uc810\uacfc \uce21\uba74\uc5d0\uc11c \uc774\ubbf8\uc9c0\ub85c \ucd2c\uc601\ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4.<\/p>\n\n\n\n

\uc608\ub97c \ub4e4\uc5b4 \ud604\ubbf8\uacbd\uc744 \uc0ac\uc6a9\ud558\uba74 2,5\ubc30 \ub610\ub294 4\ubc30 \ub300\ubb3c\ub80c\uc988\ub85c \ub9c8\uc6b0\uc2a4 \ub1cc \uc2ac\ub77c\uc774\uc2a4\ub97c \ub354 \ub113\uac8c \ubcfc \uc218 \uc788\uace0, 63\ubc30 \ub300\ubb3c\ub80c\uc988\ub97c \uc0ac\uc6a9\ud558\uba74 \uac19\uc740 \uc0d8\ud50c\uc5d0\uc11c \ud504\ub85c\ube0c\ub41c \uc561\ud2f4 \uc138\ud3ec\uace8\uaca9\uc758 \uc791\uc740 \ub514\ud14c\uc77c\uc744 \ubcfc \uc218 \uc788\uc2b5\ub2c8\ub2e4.<\/p>\n\n\n\n

\uc774\ub7ec\ud55c \ubd84\uc11d\uc744 \uac00\ub2a5\ud558\uac8c \ud558\uae30 \uc704\ud574 \uc138\ud3ec\ub098 \uc870\uc9c1\uc5d0 \uc874\uc7ac\ud558\ub294 \ud2b9\uc815 \ub2e8\ubc31\uc9c8\uc5d0 \ub300\ud55c \ud56d\uccb4\ub098 \uc5fc\ub8cc\ub97c \uc0ac\uc6a9\ud560 \uc218 \uc788\uc73c\uba70, \ud56d\uccb4\uc5d0\ub294 \uc77c\ubc18\uc801\uc73c\ub85c \ud615\uad11\ubb3c\uc9c8\uc774 \ud568\uaed8 \uc81c\uacf5\ub429\ub2c8\ub2e4.<\/p>\n\n\n\n

\uc2a4\ud1a0\ud06c\uc2a4\uc758 \uc774\ub3d9\uc740 \uc774 \ud604\uc0c1\uc744 \uc124\uba85\ud569\ub2c8\ub2e4. \ud615\uad11\uccb4\ub294 \uc5ec\uae30 \uc0c1\ud0dc\uc5d0\uc11c \uae30\uc800 \uc0c1\ud0dc\ub85c \ubc14\ub014 \ub54c \ubc29\ucd9c\ub41c \ube5b\uc758 \ud615\ud0dc\ub85c \uc9c4\ub3d9 \uc5d0\ub108\uc9c0\ub97c \uc783\uc2b5\ub2c8\ub2e4. \ud615\uad11 \ud604\ubbf8\uacbd\uc740 \ud615\uad11\uccb4\ub97c \uc5ec\uae30\uc2dc\ud0a4\ub294 \ube5b\uc744 \uc81c\uacf5\ud558\uace0 \ubc29\ucd9c\ub41c \ube5b\uc744 \uc218\uc2e0\ud569\ub2c8\ub2e4. \ubc29\ucd9c\ub41c \ube5b\uc740 \ub80c\uc988\ub85c \ud3ec\ucc29\ub418\uc5b4 CCD \uce74\uba54\ub77c \ub0b4\ubd80\uc5d0\uc11c \ucc98\ub9ac\ub41c \ud6c4 \ub514\uc9c0\ud138 \uc774\ubbf8\uc9c0\ub85c \ubcc0\ud658\ub420 \uc218 \uc788\uc2b5\ub2c8\ub2e4.<\/p>\n\n\n\n

\ud558\uc9c0\ub9cc \uc138\ud3ec \uc774\ubbf8\uc9c0 \ud68d\ub4dd\uc5d0 \ub300\ud574\uc11c\ub294 \ub098\uc911\uc5d0 \uc774\uc57c\uae30\ud558\uaca0\uc2b5\ub2c8\ub2e4. \uc774\uc81c \uc774\ubbf8\uc9c0\ub97c \uc5bb\uae30 \uc804 \uc8fc\uc694 \ub2e8\uacc4\uc5d0 \ub300\ud55c \uc608\uc2dc\uc640 \ud301\uc744 \uc18c\uac1c\ud558\uaca0\uc2b5\ub2c8\ub2e4.<\/p>\n\n\n\n

\uc138\ud3ec\ub098 \uc870\uc9c1 \ub0b4\ubd80\uc758 \uc18c\uae30\uad00\uacfc \ub2e8\ubc31\uc9c8 \uac04\uc758 \uad00\uacc4\ub97c \ubcf4\uace0 \uc774\ud574\ud558\uae30 \uc704\ud574 \ub2e4\uc591\ud55c \uc720\ud615\uc758 \uc5fc\ub8cc\uc640 \ud56d\uccb4\ub97c \uc5b4\ub5bb\uac8c \uc120\ud0dd\ud558\uace0 \uc870\ud569\ud560\uae4c\uc694?<\/p>\n\n\n\n

\uba3c\uc800 \uacfc\ud559\uc790\ub4e4\uc740 \uc5f0\uad6c \uacb0\uacfc\ub97c \ubc14\ud0d5\uc73c\ub85c \uc5b4\ub5a4 \ud56d\uccb4\uc640 \uc5fc\ub8cc\ub97c \uc0ac\uc6a9\ud560\uc9c0 \uacb0\uc815\ud574\uc57c \ud569\ub2c8\ub2e4.<\/p>\n\n\n\n

\uc608\ub97c \ub4e4\uc5b4 \uc774 \ubb38\uc11c\uc5d0\uc11c<\/a>\uba58\ub3c8\uc0ac\ub294 \uc950\ub97c \ub300\uc0c1\uc73c\ub85c \uc591\uc774\uc628\uc131 \uace0\uccb4 \uc9c0\uc9c8 \ub098\ub178\uc785\uc790(cSLN)\uc758 \ud6a8\uacfc\uc640 \uc7a0\uc7ac\uc801 \uc704\ud5d8\uc131\uc744 \ud3c9\uac00\ud558\ub824\uace0 \ud588\uc2b5\ub2c8\ub2e4. \ub9ce\uc740 \uc9c8\ubcd1\uc744 \uce58\ub8cc\ud558\uae30 \uc704\ud574 \uc57d\ubb3c\uc774\ub098 \uc720\uc804\uc790\uc758 \uc804\ub2ec\uc744 \ud5a5\uc0c1\uc2dc\ud0a4\ub294 \uac83\uc744 \ubaa9\ud45c\ub85c \ub9e4\ub144 \ub9ce\uc740 \ub098\ub178 \uc785\uc790\uac00 \uac1c\ubc1c\ub418\uace0 \uc5f0\uad6c\ub418\uace0 \uc788\uc2b5\ub2c8\ub2e4. \uc774 \uc5f0\uad6c\uc5d0\uc11c \ud765\ubbf8\ub85c\uc6b4 \uc9c8\ubb38 \uc911 \ud558\ub098\ub294 \ub098\ub178 \uc785\uc790\uac00 \ud608\uc561-\ub1cc \uc7a5\ubcbd\uc744 \ud1b5\uacfc\ud558\uc5ec \ub1cc\uc5d0 \ub3c4\ub2ec\ud560 \uc218 \uc788\ub294\uc9c0 \uc5ec\ubd80\uc600\uc2b5\ub2c8\ub2e4. \uc774 \uc7a5\ubcbd\uc740 \uc21c\ud658\ud558\ub294 \ub3c5\uc18c\ub098 \ubcd1\uc6d0\uade0\uc73c\ub85c\ubd80\ud130 \ub1cc\ub97c \ubcf4\ud638\ud558\uba70, \uc77c\ubc18\uc801\uc73c\ub85c \ubd84\uc790\uac00 \uc7a5\ubcbd\uc744 \ud1b5\uacfc\ud558\ub294 \uac83\uc740 \ubc14\ub78c\uc9c1\ud558\uc9c0 \uc54a\uc2b5\ub2c8\ub2e4. \ud558\uc9c0\ub9cc \uc774 \ud2b9\ubcc4\ud55c \uacbd\uc6b0\uc5d0\ub294 \uadf8\ub807\uc9c0 \uc54a\uc2b5\ub2c8\ub2e4, \uba58\ub3c8\uc0ac<\/a> \uc758 \ubaa9\ud45c\ub294 \ub098\ub178 \uc785\uc790\uac00 \uc7a5\ubcbd\uc744 \ud1b5\uacfc\ud558\uc5ec \ub1cc\uc5d0 \ub3c4\ub2ec\ud558\uc5ec \ud5a5\ud6c4 \uc751\uc6a9 \ubd84\uc57c\uc5d0\uc11c \uc57d\ubb3c\uc774\ub098 \uc720\uc804\uc790\ub97c \uc804\ub2ec\ud558\ub294 \uac83\uc774\uc5c8\uc2b5\ub2c8\ub2e4.<\/p>\n\n\n\n

\uc5f0\uad6c\uc9c4\uc740 \ub098\ub178 \uc785\uc790\uac00 \ub1cc \uc2e4\uc9c8\uc5d0 \uc874\uc7ac\ud558\ub294\uc9c0 \ud655\uc778\ud558\uae30 \uc704\ud574 RECA-1(\ube68\uac04\uc0c9\uc73c\ub85c \ud45c\uc2dc)\uc774\ub77c\ub294 \ud608\uad00 \ub0b4\ud53c \uc138\ud3ec \ub9c8\ucee4\ub97c \uc0ac\uc6a9\ud588\uace0, \uc138\ud3ec \ud575\uc740 \ud30c\ub780\uc0c9\uc778 DAPI(4\u2032,6-\ub514\uc544\ubbf8\ub514\ub178-2-\ud398\ub2d0\uc778\ub3cc)\ub77c\ub294 \uc5fc\ub8cc\ub85c \uc5fc\uc0c9\ud588\uc2b5\ub2c8\ub2e4. \ub610\ud55c \ud608\uad00 \uc678\ubd80\uc5d0 \uc791\uc740 \ub179\uc0c9 \uc810\uc774 \uad00\ucc30\ub418\ub294\ub370, \uc774\ub294 \ub098\ub178 \uc785\uc790\uac00 \ub1cc\uc2e4\uc9c8\uc5d0 \ub3c4\ub2ec\ud588\uc74c\uc744 \uc758\ubbf8\ud569\ub2c8\ub2e4.<\/p>\n\n\n\n

\uc544\ub798 \uc778\ud3ec\uadf8\ub798\ud53d\uc5d0\uc11c \ud45c\ud604 \uc774\ubbf8\uc9c0\uc640 \ud568\uaed8 \ud655\uc778\ud558\uc138\uc694.<\/p>\n\n\n

\n
\"Mind<\/a><\/figure><\/div>\n\n\n

RECA-1(\ube68\uac04\uc0c9)\uc5d0 \ub300\ud55c \ud56d\uccb4\uac00 \uc5b4\ub5a4 \uc5ed\ud560\uc744 \ud558\ub294\uc9c0 \uc774\ud574\ud574 \ubd05\uc2dc\ub2e4.<\/p>\n\n\n\n

\uc774\ub7ec\ud55c \ud56d\uccb4\ub294 \ud2b9\uc815 \ud504\ub85c\ube0c \uc5ed\ud560\uc744 \ud558\ub3c4\ub85d \uc124\uacc4\ub418\uc5c8\uc73c\uba70, \ud2b9\uc815 \ud56d\uc6d0(\uc774 \uacbd\uc6b0 \ub2e8\ubc31\uc9c8 RECA-1)\uc744 \ud45c\uc801\uc73c\ub85c \ud569\ub2c8\ub2e4.<\/p>\n\n\n\n

\ud615\uad11\ubb3c\uc9c8\ub85c \ub77c\ubca8\uc744 \ubd99\uc774\uac70\ub098 \ud615\uad11\ubb3c\uc9c8\uc5d0 \uc5f0\uacb0\ub41c 2\ucc28 \ud56d\uccb4\ub85c \ub098\uc911\uc5d0 \uc778\uc2dd\ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4.<\/p>\n\n\n\n

\ub530\ub77c\uc11c \uad11\uc6d0\uc73c\ub85c \uc0d8\ud50c\uc744 \uc5ec\uae30\uc2dc\ud0a8 \ud6c4, \ucc3e\uace0 \uc788\ub294 \ud2b9\uc815 \ub2e8\ubc31\uc9c8\uc740 \ud2b9\uc815 \ud30c\uc7a5\uc758 \ube5b \ubc29\ucd9c\uc744 \ud1b5\ud574 \uc0d8\ud50c\uc5d0\uc11c \uc778\uc2dd\ub429\ub2c8\ub2e4.<\/a>.<\/p>\n\n\n\n

DAPI\uc758 \uacbd\uc6b0, \uc774 \uc5fc\ub8cc\ub294 \ud575 \ubc0f \ub274\ud074\ub808\uc624\uc19c \uce74\uc6b4\ud130 \uc2a4\ud14c\uc778\uc73c\ub85c DNA\uc758 AT \uc601\uc5ed\uc5d0 \uacb0\ud569\ud560 \ub54c \ud30c\ub780\uc0c9 \ud615\uad11\uc744 \ubc1c\uc0b0\ud569\ub2c8\ub2e4.<\/p>\n\n\n\n

\uba74\uc5ed \ud615\uad11\uc744 \uc704\ud55c \ud328\ub110\uc744 \ub514\uc790\uc778\ud558\ub294 \ubc29\ubc95\uc740? <\/strong><\/p>\n\n\n\n

\ub2e4\uc74c \ub2e8\uacc4\ubd80\ud130 \uc2dc\uc791\ud558\uc138\uc694:<\/strong><\/p>\n\n\n\n

    \n
  1. \uc5f0\uad6c\uc5d0 \ud544\uc218\uc801\uc778 \ud56d\uccb4\uc640 \uc5fc\ub8cc\ub97c \uad6c\uc785(\ub610\ub294 \ube4c\ub9ac\uc138\uc694! \uacfc\ud559\uc740 \ub9e4\uc6b0 \ud611\ub825\uc801\uc774\uc5b4\uc57c \ud569\ub2c8\ub2e4!)\ud558\uc138\uc694. \ud504\ub85c\ube0c\uac00 \uc5c6\ub294 1\ucc28 \ud56d\uccb4\ub97c \uc6b0\uc120\uc801\uc73c\ub85c \uc0ac\uc6a9\ud558\uace0, 1\ucc28 \ud56d\uccb4\uc5d0\uc11c \uc219\uc8fc \uc885\uc5d0 \ud2b9\uc774\uc801\uc778 2\ucc28 \ud56d\uccb4\ub97c \uad6c\uc785\ud558\uc138\uc694. \uc608\ub97c \ub4e4\uc5b4 \ud1a0\ub07c\uc5d0\uc11c \uc0dd\uc0b0\ub41c 1\ucc28 \ud56d\uccb4\ub97c \uc0ac\uc6a9\ud558\ub294 \uacbd\uc6b0, \ud56d\ud1a0\ub07c\uc6a9 2\ucc28 \ud56d\uccb4\ub97c \uc0ac\uc6a9\ud558\uc138\uc694. \uc774\ub807\uac8c \ud558\uba74 \ud2b9\uc774\uc131\uc774 \ubcf4\uc7a5\ub429\ub2c8\ub2e4. <\/li>\n\n\n\n
  2. \ud615\uad11\ubb3c\uc9c8\ub85c \ud45c\uc9c0\ub41c 2\ucc28 \ud56d\uccb4\ub97c \uc0ac\uc6a9\ud558\uba74 1\ucc28 \ud56d\uccb4\ub2f9 \ub354 \ub9ce\uc740 \ud56d\uc6d0\uc744 \uac80\ucd9c\ud558\uc5ec \uc2e0\ud638\ub97c \ud5a5\uc0c1\uc2dc\ud0ac \uc218 \uc788\uc2b5\ub2c8\ub2e4. \ub610\ud55c \uc5f0\uad6c\uc790\uac00 \ud544\uc694\uc5d0 \ub530\ub77c \ud328\ub110\uc758 \uc0c9\uc0c1\uc744 \uc218\uc815\ud560 \uc218 \uc788\uae30 \ub54c\ubb38\uc5d0 \ub2e4\uc591\ud55c \ubd84\uc11d\uc744 \ubcf4\ub2e4 \ub3d9\uc801\uc73c\ub85c \uc815\uad50\ud558\uac8c \uc218\ud589\ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4. <\/li>\n\n\n\n
  3. \ub610 \ub2e4\ub978 \uc911\uc694\ud55c \ub2e8\uacc4\ub294 \ud604\ubbf8\uacbd \ub0b4\uc5d0\uc11c \uc5b4\ub5a4 \ud544\ud130\ub97c \uc0ac\uc6a9\ud560 \uc218 \uc788\ub294\uc9c0 \ud655\uc778\ud558\ub294 \uac83\uc785\ub2c8\ub2e4. \ud615\uad11\uccb4 \uc5ec\uae30 \ubc0f \ubc29\ucd9c \ud30c\uc7a5\uc774 \uc5ec\uae30 \ubc0f \ubc29\ucd9c \ud544\ud130 \ub0b4\ubd80\uc5d0 \uc788\ub294\uc9c0 \ud655\uc778\ud574\uc57c \ud569\ub2c8\ub2e4. \uadf8\ub807\uc9c0 \uc54a\uc73c\uba74 \ud504\ub85c\ube0c\uc5d0\uc11c \ubc29\ucd9c\uad11\uc744 \ucea1\ucc98\ud560 \uc218 \uc5c6\uc2b5\ub2c8\ub2e4. \ub2e4\uc74c\uc744 \uc0ac\uc6a9\ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4. \ud615\uad11 \uc2a4\ud399\ud2b8\ub7fc \ubdf0\uc5b4<\/a> \ub97c \ud074\ub9ad\ud558\uc5ec \ud638\ud658\uc131\uc744 \ud655\uc778\ud558\uc138\uc694.<\/li>\n\n\n\n
  4. \ubaa8\ub4e0 \ud615\uad11\uccb4\uc640 \uc5fc\ub8cc\uc758 \uc5ec\uae30 \ubc0f \ubc29\ucd9c \ud30c\uc7a5\uc774 \ub3d9\uc77c\ud55c \ubd84\uc11d \ub0b4\uc5d0\uc11c \uacb9\uce58\uc9c0 \uc54a\ub294\uc9c0 \ud655\uc778\ud569\ub2c8\ub2e4, \ud615\uad11 \uc2a4\ud399\ud2b8\ub7fc \ubdf0\uc5b4<\/a> \ub294 \ud6cc\ub96d\ud55c \uc120\ud0dd\uc785\ub2c8\ub2e4. \uc0ac\uc6a9 \uac00\ub2a5\ud55c \uac70\uc758 \ubaa8\ub4e0 \ud615\uad11\uccb4\ub97c \ucee4\ubc84\ud569\ub2c8\ub2e4!<\/li>\n<\/ol>\n\n\n\n

    \ub9c8\uc9c0\ub9c9\uc73c\ub85c, \ud575\uc0b0\uc5d0 Hoechst 33258\uc744 \uc0ac\uc6a9\ud558\uace0 RECA-1\uc5d0 \ub300\ud55c 1\ucc28 \ud56d\uccb4\uc5d0 2\ucc28 \ud56d\uccb4 Alexa Fluor 647\uc744 \ud45c\uc9c0\ud558\ub294 \uac00\uc0c1 \uc2e4\ud5d8\uc758 \uc608\ub97c \ud655\uc778\ud569\ub2c8\ub2e4.<\/p>\n\n\n\n

    \uc774\uc0c1\uc801\uc73c\ub85c\ub294 DAPI \ud050\ube0c(\uc5ec\uae30 377\/50 \ubc0f \ubc29\ucd9c 447\/60)\uc640 CY5 \ud050\ube0c(\uc5ec\uae30 628\/40 \ubc0f \ubc29\ucd9c 685\/40)\uac00 \uc788\ub294 \ud604\ubbf8\uacbd\uc744 \uc0ac\uc6a9\ud558\ub294 \uac83\uc774 \uc88b\uc2b5\ub2c8\ub2e4. \uc774 \ubaa8\ub4e0 \uc815\ubcf4\ub294 \ud615\uad11 \uc2a4\ud399\ud2b8\ub7fc \ubdf0\uc5b4<\/a> \ub97c \uc0ac\uc6a9\ud558\uc5ec \ub450 \uc5fc\ub8cc\uc758 \uc2a4\ud399\ud2b8\ub7fc\uacfc \ub450 \ud050\ube0c\uc758 \ub300\uc5ed\ud3ed\uc744 \uc5bb\uc5c8\uc2b5\ub2c8\ub2e4(\uc704\uc758 \uc778\ud3ec\uadf8\ub798\ud53d\uc5d0\uc11c \uc2a4\ud399\ud2b8\ub7fc\uc744 \ud655\uc778\ud558\uc138\uc694).<\/p>\n\n\n\n

    \uc774 \uac00\uc0c1 \uc5d0\uc138\uc774\ub294 \ud615\uad11\uccb4\uc758 \uc2a4\ud399\ud2b8\ub7fc\uc774 \uc5ec\uae30 \ubc0f \ubc29\ucd9c \ud544\ud130 \ub0b4\ubd80\uc5d0 \uc18d\ud574 \uc788\uc5b4 \uc5f0\uad6c\uc790\uac00 \uac00\ub2a5\ud55c \ucd5c\uc0c1\uc758 \ubc29\ubc95\uc73c\ub85c \uc0d8\ud50c\uc744 \ucea1\ucc98\ud560 \uc218 \uc788\ub294 \uc88b\uc740 \uc608\uc785\ub2c8\ub2e4.<\/p>\n\n\n\n

    \uc774\uc81c \uc2e4\ud5d8\uc2e4\ub85c \uac00\uc11c \ubaa8\ub4e0 \uac83\uc744 \uc2e4\ud589\uc5d0 \uc62e\uae38 \ucc28\ub840\uc785\ub2c8\ub2e4!<\/p>\n\n\n\n

    \uc774 \ud301\uc774 \ub2e4\uc74c \uc2e4\ud5d8\uc2e4 \uc2e4\ud5d8\uc5d0 \ub3c4\uc6c0\uc774 \ub418\uae38 \ubc14\ub78d\ub2c8\ub2e4. \ud589\uc6b4\uc744 \ube55\ub2c8\ub2e4!<\/p>\n\n\n\n

    \ucc38\uc870:<\/strong><\/p>\n\n\n\n

      \n
    1. \ud615\uad11 \ud604\ubbf8\uacbd \uc18c\uac1c. \ub2c8\ucf58\uc758 \ud604\ubbf8\uacbdU <\/em><\/a>https:\/\/www.microscopyu.com\/techniques\/fluorescence\/introduction-to-fluorescence-microscopy<\/a>. 2021-04-11 17:20:40\uc5d0 \uc561\uc138\uc2a4\ud588\uc2b5\ub2c8\ub2e4.<\/a><\/li>\n<\/ol>","protected":false},"excerpt":{"rendered":"

      \uacfc\ud559\uc790\uc758 \uc77c\uc0c1\uc5d0\uc11c \uac00\uc7a5 \ud070 \ubd80\ubd84\uc740 \uc2e4\ud5d8\uc744 \uc124\uacc4\ud558\uace0 \uc218\ud589\ud558\ub294 \uac83\uc785\ub2c8\ub2e4. \uc2e4\ud5d8\uc2e4 \uae30\uc220\uc758 \uc870\ud569\uc740 \uacfc\ud559\uc790\uac00 \uc81c\uc548\ud558\ub294 \ub300\ubd80\ubd84\uc758 \uc9c8\ubb38\uc5d0 \ub2f5\ud560 \uc218 \uc788\uc73c\uba70, \uc0c8\ub85c\uc6b4 \ubc29\ubc95\uc744 \uc81c\uc548\ud558\ub294 \uc791\uc5c5 \ud750\ub984\uc740 \uacfc\ud559\uc790\uc758 \ubc30\uacbd\uacfc \uacbd\ud5d8\uc5d0 \ub530\ub77c \ub2ec\ub77c\uc9d1\ub2c8\ub2e4. \uc0dd\ubb3c\ud559\uc790\uc5d0\uac8c \uc138\ud3ec \uc774\ubbf8\uc9c0\ub294 [...]\uc5d0 \ubb34\uc2a8 \uc77c\uc774 \uc77c\uc5b4\ub098\uace0 \uc788\ub294\uc9c0\uc5d0 \ub300\ud574 \ub9ce\uc740 \uac83\uc744 \ub9d0\ud574\uc904 \uc218 \uc788\uc2b5\ub2c8\ub2e4.<\/p>","protected":false},"author":18,"featured_media":12982,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[66,959,958,28,38],"tags":[51,554,962],"yoast_head":"\nMicroscopy: How Scientists Use Fluorescence To See Inside Cells -<\/title>\n<meta name=\"description\" content=\"Fluorescence microscopy can say a lot about a cell process through image. That is why knowing how to design the experiment is so important.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mindthegraph.com\/blog\/ko\/\ud604\ubbf8\uacbd-\uacfc\ud559\uc790\ub4e4\uc774-\ud615\uad11\uc744-\uc0ac\uc6a9\ud558\uc5ec-\uc138\ud3ec-\ub0b4\ubd80\ub97c-\ubcf4\/\" \/>\n<meta property=\"og:locale\" content=\"ko_KR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Microscopy: How Scientists Use Fluorescence To See Inside Cells\" \/>\n<meta property=\"og:description\" content=\"Fluorescence microscopy can say a lot about a cell process through image. That is why knowing how to design the experiment is so important.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/mindthegraph.com\/blog\/ko\/\ud604\ubbf8\uacbd-\uacfc\ud559\uc790\ub4e4\uc774-\ud615\uad11\uc744-\uc0ac\uc6a9\ud558\uc5ec-\uc138\ud3ec-\ub0b4\ubd80\ub97c-\ubcf4\/\" \/>\n<meta property=\"og:site_name\" content=\"Mind the Graph Blog\" \/>\n<meta property=\"article:published_time\" content=\"2021-06-08T23:10:05+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2023-01-05T17:22:32+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/06\/microscopy-facebook.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1200\" \/>\n\t<meta property=\"og:image:height\" content=\"628\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Bruna Soldera\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:title\" content=\"Microscopy: How Scientists Use Fluorescence To See Inside Cells\" \/>\n<meta name=\"twitter:image\" content=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/06\/microscopy-twitter.jpg\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Bruna Soldera\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"5 minutes\" \/>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Microscopy: How Scientists Use Fluorescence To See Inside Cells -","description":"Fluorescence microscopy can say a lot about a cell process through image. 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