{"id":12979,"date":"2021-06-08T20:10:05","date_gmt":"2021-06-08T23:10:05","guid":{"rendered":"https:\/\/mindthegraph.com\/blog\/?p=12979"},"modified":"2023-01-05T14:22:32","modified_gmt":"2023-01-05T17:22:32","slug":"microscopy-how-scientists-use-fluorescence-to-see-inside-cells","status":"publish","type":"post","link":"https:\/\/mindthegraph.com\/blog\/ja\/%e9%a1%95%e5%be%ae%e9%8f%a1%e6%b3%95-%e8%9b%8d%e5%85%89%e3%81%ab%e3%82%88%e3%82%8b%e7%b4%b0%e8%83%9e%e5%86%85%e3%81%ae%e8%a6%b3%e5%af%9f\/","title":{"rendered":"\u30de\u30a4\u30af\u30ed\u30b9\u30b3\u30fc\u30d7\u7d30\u80de\u306e\u4e2d\u3092\u898b\u308b\u305f\u3081\u306b\u86cd\u5149\u3092\u4f7f\u3046\u79d1\u5b66\u8005\u305f\u3061"},"content":{"rendered":"

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\n
\"Mind<\/a><\/figure><\/div>\n\n\n

RECA-1\u6297\u4f53\uff08\u8d64\u8272\uff09\u304c\u4f55\u3092\u3057\u3066\u3044\u308b\u306e\u304b\u3001\u7406\u89e3\u3057\u307e\u3057\u3087\u3046\u3002<\/p>\n\n\n\n

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DAPI\u306e\u5834\u5408\u3001\u3053\u306e\u8272\u7d20\u306f\u6838\u3084\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u30ab\u30a6\u30f3\u30bf\u30fc\u30b9\u30c6\u30a4\u30f3\u3067\u3042\u308a\u3001DNA\u306eAT\u9818\u57df\u306b\u7d50\u5408\u3059\u308b\u3068\u9752\u3044\u86cd\u5149\u3092\u767a\u3059\u308b\u3002<\/p>\n\n\n\n

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\u307e\u305a\u306f\u3053\u306e\u624b\u9806\u3067\u59cb\u3081\u3066\u307f\u3066\u304f\u3060\u3055\u3044\u3002<\/strong><\/p>\n\n\n\n

    \n
  1. \u7814\u7a76\u306b\u5fc5\u8981\u306a\u6297\u4f53\u3084\u8272\u7d20\u3092\u8cfc\u5165\u3059\u308b\uff08\u307e\u305f\u306f\u501f\u308a\u308b\uff01\u79d1\u5b66\u306f\u975e\u5e38\u306b\u5354\u529b\u7684\u3067\u3042\u308b\u3079\u304d\u3060\uff01\uff09\u3002\u4e00\u6b21\u6297\u4f53\uff08\u30d7\u30ed\u30fc\u30d6\u306a\u3057\uff09\u3092\u512a\u5148\u3057\u3001\u4e00\u6b21\u6297\u4f53\u304b\u3089\u5bbf\u4e3b\u7a2e\u306b\u7279\u7570\u7684\u306a\u4e8c\u6b21\u6297\u4f53\u3092\u8cfc\u5165\u3059\u308b\u3002\u4f8b\u3048\u3070\u3001\u30a6\u30b5\u30ae\u3067\u7523\u751f\u3055\u308c\u305f\u4e00\u6b21\u6297\u4f53\u3092\u4f7f\u7528\u3059\u308b\u5834\u5408\u306f\u3001\u6297\u30a6\u30b5\u30ae\u7528\u306e\u4e8c\u6b21\u6297\u4f53\u3092\u4f7f\u7528\u3059\u308b\u3002\u3053\u308c\u306b\u3088\u308a\u7279\u7570\u6027\u304c\u4fdd\u8a3c\u3055\u308c\u307e\u3059\u3002 <\/li>\n\n\n\n
  2. \u86cd\u5149\u8272\u7d20\u3067\u6a19\u8b58\u3055\u308c\u305f\u4e8c\u6b21\u6297\u4f53\u3092\u4f7f\u7528\u3059\u308b\u3068\u3001\u4e00\u6b21\u6297\u4f53\u3042\u305f\u308a\u3088\u308a\u591a\u304f\u306e\u6297\u539f\u3092\u691c\u51fa\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u3001\u30b7\u30b0\u30ca\u30eb\u304c\u5897\u5f37\u3055\u308c\u307e\u3059\u3002\u307e\u305f\u3001\u7814\u7a76\u8005\u306e\u30cb\u30fc\u30ba\u306b\u5fdc\u3058\u3066\u30d1\u30cd\u30eb\u306e\u8272\u3092\u5909\u66f4\u3067\u304d\u308b\u305f\u3081\u3001\u69d8\u3005\u306a\u30a2\u30c3\u30bb\u30a4\u3092\u7cbe\u7dfb\u306b\u884c\u3046\u3053\u3068\u304c\u3067\u304d\u308b\u3001\u3088\u308a\u30c0\u30a4\u30ca\u30df\u30c3\u30af\u306a\u65b9\u6cd5\u3068\u8a00\u3048\u307e\u3059\u3002 <\/li>\n\n\n\n
  3. \u3082\u3046\u4e00\u3064\u306e\u91cd\u8981\u306a\u30b9\u30c6\u30c3\u30d7\u306f\u3001\u4f7f\u7528\u3059\u308b\u9855\u5fae\u93e1\u306b\u3069\u306e\u3088\u3046\u306a\u30d5\u30a3\u30eb\u30bf\u30fc\u304c\u3042\u308b\u306e\u304b\u3092\u78ba\u8a8d\u3059\u308b\u3053\u3068\u3067\u3059\u3002\u86cd\u5149\u4f53\u306e\u52b1\u8d77\u6ce2\u9577\u3068\u767a\u5149\u6ce2\u9577\u304c\u52b1\u8d77\u30fb\u767a\u5149\u30d5\u30a3\u30eb\u30bf\u30fc\u5185\u306b\u3042\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u3001\u305d\u3046\u3067\u306a\u3044\u5834\u5408\u306f\u30d7\u30ed\u30fc\u30d6\u304b\u3089\u306e\u767a\u5149\u5149\u3092\u6355\u3089\u3048\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u305b\u3093\u3002\u4f7f\u7528\u3067\u304d\u308b\u306e\u306f \u86cd\u5149\u30b9\u30da\u30af\u30c8\u30eb\u30d3\u30e5\u30a2\u30fc<\/a> \u3092\u30af\u30ea\u30c3\u30af\u3057\u3066\u4e92\u63db\u6027\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/li>\n\n\n\n
  4. \u540c\u3058\u30a2\u30c3\u30bb\u30a4\u5185\u3067\u3001\u3059\u3079\u3066\u306e\u86cd\u5149\u4f53\u3084\u8272\u7d20\u306e\u52b1\u8d77\u6ce2\u9577\u3068\u767a\u5149\u6ce2\u9577\u304c\u91cd\u306a\u3089\u306a\u3044\u3088\u3046\u306b\u3059\u308b\u3053\u3068\u3002 \u86cd\u5149\u30b9\u30da\u30af\u30c8\u30eb\u30d3\u30e5\u30a2\u30fc<\/a> \u304c\u304a\u3059\u3059\u3081\u3067\u3059\u3002\u307b\u307c\u5168\u3066\u306e\u86cd\u5149\u4f53\u3092\u7db2\u7f85\u3057\u3066\u3044\u307e\u3059!<\/li>\n<\/ol>\n\n\n\n

    \u6700\u5f8c\u306b\u3001\u6838\u9178\u306bHoechst 33258\u3001RECA-1\u306b\u5bfe\u3059\u308b\u4e00\u6b21\u6297\u4f53\u306b\u4e8c\u6b21\u6297\u4f53Alexa Fluor 647\u3092\u6a19\u8b58\u3057\u305f\u4eee\u60f3\u5b9f\u9a13\u306e\u4f8b\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n

    DAPI\u30ad\u30e5\u30fc\u30d6\uff08\u52b1\u8d77377\/50\u3001\u767a\u5149447\/60\uff09\u3068CY5\u30ad\u30e5\u30fc\u30d6\uff08\u52b1\u8d77628\/40\u3001\u767a\u5149685\/40\uff09\u3092\u5099\u3048\u305f\u9855\u5fae\u93e1\u3092\u4f7f\u7528\u3059\u308b\u3053\u3068\u304c\u7406\u60f3\u7684\u3067\u3059\u3002\u3053\u308c\u3089\u306e\u60c5\u5831\u306f\u3059\u3079\u3066 \u86cd\u5149\u30b9\u30da\u30af\u30c8\u30eb\u30d3\u30e5\u30a2\u30fc<\/a> \u3092\u4f5c\u6210\u3057\u3001\u4e21\u65b9\u306e\u8272\u7d20\u306e\u30b9\u30da\u30af\u30c8\u30eb\u3068\u3001\u4e21\u65b9\u306e\u30ad\u30e5\u30fc\u30d6\u306e\u5e2f\u57df\u5e45\u3092\u53d6\u5f97\u3057\u307e\u3057\u305f\uff08\u4e0a\u306e\u30a4\u30f3\u30d5\u30a9\u30b0\u30e9\u30d5\u30a3\u30c3\u30af\u3067\u30b9\u30da\u30af\u30c8\u30eb\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\uff09\u3002<\/p>\n\n\n\n

    \u3053\u306e\u4eee\u8aac\u306e\u30a8\u30c3\u30bb\u30a4\u306f\u3001\u86cd\u5149\u4f53\u306e\u30b9\u30da\u30af\u30c8\u30eb\u304c\u52b1\u8d77\u3068\u767a\u5149\u306e\u30d5\u30a3\u30eb\u30bf\u30fc\u306e\u4e2d\u306b\u5c5e\u3057\u3066\u3044\u308b\u826f\u3044\u4f8b\u3067\u3001\u7814\u7a76\u8005\u304c\u81ea\u5206\u306e\u30b5\u30f3\u30d7\u30eb\u3092\u6700\u9069\u306a\u65b9\u6cd5\u3067\u6355\u3089\u3048\u308b\u3053\u3068\u3092\u53ef\u80fd\u306b\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n

    \u3055\u3042\u3001\u3044\u3088\u3044\u3088\u7814\u7a76\u5ba4\u3067\u3001\u3059\u3079\u3066\u3092\u5b9f\u8df5\u3057\u3066\u307f\u307e\u3057\u3087\u3046<\/p>\n\n\n\n

    \u3053\u308c\u3089\u306e\u30d2\u30f3\u30c8\u304c\u3001\u3042\u306a\u305f\u306e\u6b21\u306e\u30e9\u30dc\u5b9f\u9a13\u306b\u5f79\u7acb\u3064\u3053\u3068\u3092\u9858\u3063\u3066\u3044\u307e\u3059\u3002\u5e78\u904b\u3092\u7948\u308a\u307e\u3059\u3002<\/p>\n\n\n\n

    \u53c2\u8003\u6587\u732e<\/strong><\/p>\n\n\n\n

      \n
    1. \u86cd\u5149\u9855\u5fae\u93e1\u5165\u9580\u3002 \u30cb\u30b3\u30f3\u306e\u30de\u30a4\u30af\u30ed\u30b9\u30b3\u30fc\u30d7U <\/em><\/a>https:\/\/www.microscopyu.com\/techniques\/fluorescence\/introduction-to-fluorescence-microscopy<\/a>.\u30a2\u30af\u30bb\u30b9\u6570 2021-04-11 17:20:40.<\/a><\/li>\n<\/ol>","protected":false},"excerpt":{"rendered":"

      \u79d1\u5b66\u8005\u306e\u30eb\u30fc\u30c6\u30a3\u30f3\u306e\u5927\u90e8\u5206\u306f\u3001\u5b9f\u9a13\u3092\u8a08\u753b\u3057\u3001\u5b9f\u884c\u3059\u308b\u3053\u3068\u3067\u3059\u3002\u5b9f\u9a13\u6280\u8853\u306e\u7d44\u307f\u5408\u308f\u305b\u306b\u3088\u3063\u3066\u3001\u79d1\u5b66\u8005\u304c\u63d0\u6848\u3059\u308b\u7591\u554f\u306e\u307b\u3068\u3093\u3069\u306b\u7b54\u3048\u308b\u3053\u3068\u304c\u3067\u304d\u3001\u65b0\u3057\u3044\u65b9\u6cd5\u3092\u63d0\u6848\u3059\u308b\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u306f\u3001\u79d1\u5b66\u8005\u306e\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u3068\u7d4c\u9a13\u306b\u3088\u3063\u3066\u6c7a\u307e\u308a\u307e\u3059\u3002\u751f\u7269\u5b66\u8005\u306b\u3068\u3063\u3066\u3001\u7d30\u80de\u306e\u753b\u50cf\u306f\u3001\u5f7c\u3089\u304c\u7814\u7a76\u3057\u3066\u3044\u308b\u30d7\u30ed\u30bb\u30b9\u3084\u30e1\u30ab\u30cb\u30ba\u30e0\u3067\u4f55\u304c\u8d77\u3053\u3063\u3066\u3044\u308b\u304b\u306b\u3064\u3044\u3066\u591a\u304f\u3092\u8a9e\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u5149\u5b66\u9855\u5fae\u93e1\u306f\u3001\u751f\u7269\u79d1\u5b66\u306b\u304a\u3044\u3066\u975e\u5e38\u306b\u5e83\u304f\u666e\u53ca\u3057\u3066\u3044\u308b\u6280\u8853\u3067\u3059\u3002\u67d3\u6599\u3001\u6297\u4f53\u3001\u86cd\u5149\u30d7\u30ed\u30fc\u30d6\u3092\u4f7f\u7528\u3059\u308b\u3053\u3068\u3067\u3001\u79d1\u5b66\u8005\u306f\u9855\u5fae\u93e1\u306e\u7d30\u80de\u5185\u306b\u3001\u5f93\u6765\u306f\u5c0f\u3055\u3059\u304e\u3066\u898b\u308b\u3053\u3068\u3082\u7406\u89e3\u3059\u308b\u3053\u3068\u3082\u3067\u304d\u306a\u304b\u3063\u305f\u3082\u306e\u306e\u753b\u50cf\u3092\u898b\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u3088\u3046\u306b\u306a\u308b\u306e\u3067\u3059\u3002\u86cd\u5149\u9855\u5fae\u93e1\u3068\u86cd\u5149\u8272\u7d20\u306e\u4f7f\u7528\u306f\u3001[...]\u3067\u53ef\u80fd\u306b\u306a\u308a\u307e\u3057\u305f\u3002<\/p>","protected":false},"author":18,"featured_media":12982,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[66,959,958,28,38],"tags":[51,554,962],"yoast_head":"\nMicroscopy: How Scientists Use Fluorescence To See Inside Cells -<\/title>\n<meta name=\"description\" content=\"Fluorescence microscopy can say a lot about a cell process through image. That is why knowing how to design the experiment is so important.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mindthegraph.com\/blog\/ja\/\u9855\u5fae\u93e1\u6cd5-\u86cd\u5149\u306b\u3088\u308b\u7d30\u80de\u5185\u306e\u89b3\u5bdf\/\" \/>\n<meta property=\"og:locale\" content=\"ja_JP\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Microscopy: How Scientists Use Fluorescence To See Inside Cells\" \/>\n<meta property=\"og:description\" content=\"Fluorescence microscopy can say a lot about a cell process through image. That is why knowing how to design the experiment is so important.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/mindthegraph.com\/blog\/ja\/\u9855\u5fae\u93e1\u6cd5-\u86cd\u5149\u306b\u3088\u308b\u7d30\u80de\u5185\u306e\u89b3\u5bdf\/\" \/>\n<meta property=\"og:site_name\" content=\"Mind the Graph Blog\" \/>\n<meta property=\"article:published_time\" content=\"2021-06-08T23:10:05+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2023-01-05T17:22:32+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/06\/microscopy-facebook.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1200\" \/>\n\t<meta property=\"og:image:height\" content=\"628\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Bruna Soldera\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:title\" content=\"Microscopy: How Scientists Use Fluorescence To See Inside Cells\" \/>\n<meta name=\"twitter:image\" content=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/06\/microscopy-twitter.jpg\" \/>\n<meta name=\"twitter:label1\" content=\"\u57f7\u7b46\u8005\" \/>\n\t<meta name=\"twitter:data1\" content=\"Bruna Soldera\" \/>\n\t<meta name=\"twitter:label2\" content=\"\u63a8\u5b9a\u8aad\u307f\u53d6\u308a\u6642\u9593\" \/>\n\t<meta name=\"twitter:data2\" content=\"5\u5206\" \/>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Microscopy: How Scientists Use Fluorescence To See Inside Cells -","description":"Fluorescence microscopy can say a lot about a cell process through image. 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