{"id":16636,"date":"2022-09-10T18:27:00","date_gmt":"2022-09-10T21:27:00","guid":{"rendered":"https:\/\/mindthegraph.com\/blog\/?p=16636"},"modified":"2023-03-17T14:47:45","modified_gmt":"2023-03-17T17:47:45","slug":"success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids","status":"publish","type":"post","link":"https:\/\/mindthegraph.com\/blog\/hu\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/","title":{"rendered":"FGF jel\u00e1tvitel a vastagb\u00e9lr\u00e1k organoidok \u00f6nmeg\u00faj\u00edt\u00e1s\u00e1ban Paper Review"},"content":{"rendered":"

A betegekt\u0151l sz\u00e1rmaz\u00f3 CRC-sejtekben az FGFR-g\u00e1tl\u00e1s hat\u00e1s\u00e1ra kialakul\u00f3 fogyat\u00e9kos szferoid- \u00e9s organoid-k\u00e9pz\u0151d\u00e9s sokkal k\u00f6nnyebben \u00e9rthet\u0151 az Mind the Graph illusztr\u00e1ci\u00f3inak seg\u00edts\u00e9g\u00e9vel. <\/p>\n\n\n\n

A szerz\u0151 \u00e1ltal k\u00e9sz\u00edtett s\u00e9ma azt mutatja, hogy a seb\u00e9szeti biopszi\u00e1kat enzimatikusan felt\u00e1rt\u00e1k, hogy az egyes sejtek szuszpenzi\u00f3j\u00e1t nyerj\u00e9k. A naiv, v\u00e1logatatlan sejteket FGF2-tartalm\u00fa CSC-k\u00f6zegben vagy az SU-5402 FGFR-inhibitorral teny\u00e9sztett\u00e9k.<\/p>\n\n\n\n

Vizsg\u00e1lt\u00e1k a fibroblaszt n\u00f6veked\u00e9si faktor 2 (FGF2) szerep\u00e9t a vastagb\u00e9lr\u00e1k \u0151ssejtjeinek \u00f6nmeg\u00fajul\u00e1s\u00e1ban.<\/strong><\/h2>\n\n\n\n

Az \"FGF jel\u00e1tvitel a vastagb\u00e9lr\u00e1k-organoidok \u00f6nmeg\u00faj\u00edt\u00e1s\u00e1ban\" c\u00edm\u0171 tanulm\u00e1ny\u00e1ban J\u00f6rg Otte \u00e9s munkat\u00e1rsai (2019) arr\u00f3l sz\u00e1moltak be, hogy amikor a naiv egyes sejteket Matrigelbe \u00e1gyazt\u00e1k \u00e9s CSC-k\u00f6zegben teny\u00e9sztett\u00e9k, minden elemzett betegn\u00e9l \u00f6sszetettebb \u00f6nszervez\u0151d\u00e9st figyeltek meg. <\/p>\n\n\n\n

Az EMT-hez k\u00f6zvetlen\u00fcl kapcsol\u00f3d\u00f3 g\u00e9nek, valamint a mesenchym\u00e1lis \u00e9s \u00f6nmeg\u00faj\u00edt\u00f3 fenot\u00edpus (TGFB3, ID1, ID2 \u00e9s ID4) az organoidokban t\u00falreprezent\u00e1ltnak bizonyultak. <\/p>\n\n\n\n

M\u00edg az FGFR-g\u00e1tl\u00e1s a MAPK jel\u00e1tvitel fokoz\u00f3d\u00e1s\u00e1hoz vezetett, addig az induk\u00e1lt differenci\u00e1l\u00f3d\u00e1st, a legt\u00f6bb \u0151ssejtmarker elveszt\u00e9s\u00e9t \u00e9s epiteli\u00e1lis fenot\u00edpust figyeltek meg. <\/p>\n\n\n\n

A r\u00e1kos \u0151ssejtek a rosszindulat\u00fa sejtek olyan alpopul\u00e1ci\u00f3ja, amely k\u00e9pes az \u00f6nmeg\u00fajul\u00e1sra \u00e9s a differenci\u00e1lt tumorsejtek folyamatos forr\u00e1sak\u00e9nt szolg\u00e1l. A CSC-kult\u00fara egyes protokolljaiban a fibroblaszt n\u00f6veked\u00e9si faktor 2 fontos \u00f6sszetev\u0151je az \u0151ssejtek fenntart\u00e1s\u00e1nak. <\/p>\n\n\n\n

A CSC-k\u00f6zegben teny\u00e9sztett organoidokban viszont kimutatt\u00e1k a TGF-\u03b2 \u00e9s a differenci\u00e1l\u00f3d\u00e1st g\u00e1tl\u00f3 g\u00e9ncsal\u00e1d mind a n\u00e9gy tagj\u00e1nak indukci\u00f3j\u00e1t.<\/p>\n\n\n\n

A kutat\u00e1sban 9 egym\u00e1st k\u00f6vet\u0151, nem v\u00e1logatott CRC-beteg vett r\u00e9szt.<\/strong><\/p>\n\n\n\n

Ellen\u0151rizze a eredeti cikk itt<\/a>.<\/p>\n\n\n\n

Grafikus absztrakt az Mind the Graph seg\u00edts\u00e9g\u00e9vel<\/strong><\/h2>\n\n\n\n
<\/div>\n\n\n
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K\u00e9sz\u00fclt Mind the Graph<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

N\u00e9h\u00e1ny k\u00f6vetkeztet\u00e9s\u00fck potenci\u00e1lisan meger\u0151s\u00edti azt, amit kor\u00e1bban tudtunk err\u0151l a ter\u00fcletr\u0151l: \"A pluripotenci\u00e1val kapcsolatos NANOG, LIN28 \u00e9s SALL1 g\u00e9neket kiz\u00e1r\u00f3lag a hESC-k fejezik ki. A \"BMP jel\u00e1tviteli \u00fatvonal negat\u00edv szab\u00e1lyoz\u00e1sa\" \u00e9s a \"SMAD feh\u00e9rje jel\u00e1tvitel\" GO-terminusokat detekt\u00e1ltuk a hESC-kult\u00far\u00e1nkban\" - javasolta Otte.<\/p>\n\n\n\n

A szerz\u0151k elismerik, hogy \"ennek a g\u00e9nk\u00e9szletnek a GO-termin\u00e1lis elemz\u00e9se csak n\u00e9h\u00e1ny szignifik\u00e1ns eredm\u00e9nyt hozott a gyakran szab\u00e1lyozott g\u00e9nek alacsony sz\u00e1ma miatt. A CSC-k\u00f6zepes \u00e1llapotra vonatkoz\u00f3 GO-terminusok t\u00f6bbs\u00e9ge akt\u00edv transzkripci\u00f3val, sejtprolifer\u00e1ci\u00f3val vagy fokozott gl\u00fck\u00f3zfelv\u00e9tellel volt kapcsolatos. <\/p>\n\n\n\n

Az FGFR-g\u00e1tl\u00e1s ut\u00e1n sejtciklus-szab\u00e1lyoz\u00f3kat, valamint sz\u00e1mos olyan g\u00e9nt tal\u00e1ltunk, amelyeket a \"h\u00e1msejtek differenci\u00e1l\u00f3d\u00e1s\u00e1val\", az \"em\u00e9szt\u00e9ssel\" vagy az \"epesav- \u00e9s epes\u00f3sz\u00e1ll\u00edt\u00e1ssal\" annot\u00e1ltak.\"\"<\/p>\n\n\n\n

Pap\u00edrok hat\u00e1sa \u00e9s teljes\u00edtm\u00e9nye<\/strong><\/h2>\n\n\n\n

Elemezhetj\u00fck a Altmetric<\/a> adatok, hogy a lap online figyelem pontsz\u00e1ma magasabb mint a 68%<\/strong><\/strong> a Scientific Reports \u00f6sszes t\u00f6bbi hasonl\u00f3 kor\u00fa cikk\u00e9t.<\/strong><\/p>\n\n\n\n

<\/div>\n\n\n
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\"\"<\/a>
Forr\u00e1s Altmetrics<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

Jav\u00edtsa cikke el\u00e9rhet\u0151s\u00e9g\u00e9t az infografik\u00e1k erej\u00e9vel is.<\/p>\n\n\n\n

\n
Pr\u00f3b\u00e1lja ki az Mind the Graph-t<\/strong><\/a><\/div>\n<\/div>\n\n\n\n
<\/div>","protected":false},"excerpt":{"rendered":"

A betegekt\u0151l sz\u00e1rmaz\u00f3 CRC-sejtekben az FGFR-g\u00e1tl\u00e1s hat\u00e1s\u00e1ra kialakul\u00f3 fogyat\u00e9kos szferoid- \u00e9s organoid-k\u00e9pz\u0151d\u00e9s az \u00e1br\u00e1ink seg\u00edts\u00e9g\u00e9vel k\u00f6nnyebben \u00e9rthet\u0151.<\/p>","protected":false},"author":29,"featured_media":20014,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[195,958,957,1],"tags":[96,554,775,963],"yoast_head":"\nFGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog<\/title>\n<meta name=\"description\" content=\"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mindthegraph.com\/blog\/hu\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/\" \/>\n<meta property=\"og:locale\" content=\"hu_HU\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - 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