{"id":12130,"date":"2021-01-13T09:30:00","date_gmt":"2021-01-13T11:30:00","guid":{"rendered":"https:\/\/mindthegraph.com\/blog\/?p=12130"},"modified":"2022-10-18T08:09:26","modified_gmt":"2022-10-18T11:09:26","slug":"pyscratch-spatial-and-temporal-information-from-scratch-assays","status":"publish","type":"post","link":"https:\/\/mindthegraph.com\/blog\/hu\/pyscratch-ter-es-tempo-informacio-az-osszekarcolasi-probak\/","title":{"rendered":"PyScratch: Scratch Assays t\u00e9rbeli \u00e9s id\u0151beli inform\u00e1ci\u00f3inak feldolgoz\u00e1s\u00e1ra szolg\u00e1l\u00f3 \u00faj eszk\u00f6z"},"content":{"rendered":"<p>A PyScratch egy \u00faj, ny\u00edlt forr\u00e1sk\u00f3d\u00fa, Python nyelven implement\u00e1lt szoftver a migr\u00e1ci\u00f3s adatok elemz\u00e9s\u00e9hez, amely felhaszn\u00e1l\u00f3bar\u00e1t fel\u00fclettel rendelkezik, amely lehet\u0151v\u00e9 teszi a kev\u00e9s vagy semmilyen programoz\u00e1si ismerettel nem rendelkez\u0151 tud\u00f3sok sz\u00e1m\u00e1ra a haszn\u00e1lat\u00e1t. <\/p>\n\n\n\n<p>A szoftvert a Campinasi Egyetem NanoCell Interactions laborat\u00f3rium\u00e1nak h\u00e1rom brazil tud\u00f3s\u00e1nak egy\u00fcttm\u0171k\u00f6d\u00e9s\u00e9ben tervezt\u00e9k 2017-ben. <\/p>\n\n\n\n<p>Ezeknek a tud\u00f3soknak a sz\u00fcks\u00e9gszer\u0171s\u00e9g\u00e9b\u0151l sz\u00fcletett, \u00e9s ma a szoftver c\u00e9lja a kutat\u00f3k napi gyakorlat\u00e1nak megk\u00f6nny\u00edt\u00e9se, a k\u00e9zi elemz\u00e9s kiz\u00e1r\u00e1sa, a reproduk\u00e1lhat\u00f3s\u00e1g n\u00f6vel\u00e9se \u00e9s az emberi hib\u00e1k minimaliz\u00e1l\u00e1sa.<\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter size-large\"><a href=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/01\/pyscratch.png\"><img decoding=\"async\" loading=\"lazy\" width=\"1024\" height=\"551\" src=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/01\/pyscratch-1024x551.png\" alt=\"\" class=\"wp-image-12131\" srcset=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/01\/pyscratch-1024x551.png 1024w, https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/01\/pyscratch-300x161.png 300w, https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/01\/pyscratch-768x413.png 768w, https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/01\/pyscratch.png 1183w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/a><\/figure><\/div>\n\n\n\n<p>A k\u00e9pelemz\u00e9s az egyik legfontosabb m\u00f3dszer, amelyet a tud\u00f3sok k\u00fcl\u00f6nb\u00f6z\u0151 m\u00f3dszertanokban haszn\u00e1lnak az eredm\u00e9nyek elemz\u00e9s\u00e9re. <\/p>\n\n\n\n<p>Az elm\u00falt \u00e9vekben nemcsak az automatiz\u00e1lt mikroszk\u00f3pok haszn\u00e1lata n\u0151tt, hanem a megszerzett adatok \u00f6sszetetts\u00e9ge is. <\/p>\n\n\n\n<p>A tud\u00f3sok munk\u00e1j\u00e1nak r\u00e9sze, hogy felfedezz\u00e9k, hogyan kezeljenek egy \u00fajfajta inform\u00e1ci\u00f3t, emellett elemezz\u00e9k \u00e9s feldolgozz\u00e1k az adatokat. <\/p>\n\n\n\n<p>Ehhez a tud\u00f3soknak j\u00f3 \u00e9s speci\u00e1lis eszk\u00f6z\u00f6kre van sz\u00fcks\u00e9g\u00fck, hogy az \u00f6sszes adatot megfelel\u0151en ki tudj\u00e1k nyerni \u00e9s \u00e9rtelmezni tudj\u00e1k.<\/p>\n\n\n\n<p>A PyScratch-ot el\u0151sz\u00f6r az\u00e9rt hozt\u00e1k l\u00e9tre, hogy seg\u00edtsen Fernanda Garcia-Fossa biol\u00f3gus kutat\u00f3nak a migr\u00e1ci\u00f3s vizsg\u00e1latokb\u00f3l sz\u00e1rmaz\u00f3 hatalmas mennyis\u00e9g\u0171 adat elemz\u00e9s\u00e9ben. <\/p>\n\n\n\n<p>\"Elv\u00e9geztem a r\u00e1kos sejtek vakar\u00e1s\u00e1t, \u00e9s 48 \u00f3r\u00e1n \u00e1t inkub\u00e1ltam a berendez\u00e9sben, 15 percenk\u00e9nt k\u00e9peket k\u00e9sz\u00edtettem, \u00e9s csak egy k\u00eds\u00e9rlet v\u00e9g\u00e9re k\u00f6r\u00fclbel\u00fcl ezer k\u00e9pem volt, amit megn\u00e9zhettem \u00e9s elemezhettem! <\/p>\n\n\n\n<p>K\u00e9ptelens\u00e9g volt k\u00e9zzel elv\u00e9gezni\" - mondja Garcia-Fossa. A probl\u00e9ma megold\u00e1s\u00e1hoz Garcia-Fossa a t\u00e1rs\u00e1hoz, a fizikus Vladimir Gaalhoz fordult seg\u00edts\u00e9g\u00e9rt, aki abban az id\u0151ben \u00e9ppen Pythont tanult, ami remek lehet\u0151s\u00e9g volt arra, hogy ezt a tud\u00e1st a gyakorlatba \u00fcltesse.<\/p>\n\n\n\n<p>Teh\u00e1t mindketten dolgoztak a megold\u00e1son egy Python rutinon kereszt\u00fcl, amely felismerte a karcolt ter\u00fcleteket, \u00e9s egy csv f\u00e1jlba export\u00e1lta. <\/p>\n\n\n\n<p>\"Az id\u0151, \u00e9rezt\u00fck a sz\u00fcks\u00e9gess\u00e9g\u00e9t, hogy dolgozzon ki egy felhaszn\u00e1l\u00f3i fel\u00fclet, \u00edgy m\u00e9g k\u00f6nnyebben haszn\u00e1lhat\u00f3, \u00edgy tudtuk k\u00f6zz\u00e9tenni a szoftvert minden kutat\u00f3, hogy sz\u00fcks\u00e9g van a haszn\u00e1latra is\", mondja Garcia-Fossa a szoftver cikket, hogy akkor n\u00e9zd meg, hogy ki<a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0169260719313914\"> ide kattintva<\/a>.<\/p>\n\n\n\n<p>Garcia-Fossa azt is elmondja, hogy sok\u00e1ig tartott, am\u00edg a k\u00e9pek alapj\u00e1n felismert\u00e9k \u00e9s meghat\u00e1rozt\u00e1k a migr\u00e1ci\u00f3s ter\u00fcletet, mivel a k\u00e9pek a f\u00e9ny, a f\u00f3kusz \u00e9s a kontraszt miatt nagyon elt\u00e9rhetnek egym\u00e1st\u00f3l, \u00e9s a ma haszn\u00e1lt verzi\u00f3 m\u00e1r el\u00e9g j\u00f3l elemez. <\/p>\n\n\n\n<p>Mindazon\u00e1ltal m\u00e9g mindig dolgoznak a szoftveren, \u00faj \u00e9s jobb verzi\u00f3kat ind\u00edtva, mivel a k\u00f6zz\u00e9tett cikk a felhaszn\u00e1l\u00f3k ig\u00e9nyei miatt felvetett n\u00e9h\u00e1ny fejleszt\u00e9si ig\u00e9nyt.<\/p>\n\n\n\n<p>A szoftver teljes\u00edtm\u00e9ny\u00e9nek valid\u00e1l\u00e1s\u00e1ra haszn\u00e1lt pr\u00f3ba, a migr\u00e1ci\u00f3s pr\u00f3ba, vagy karcol\u00e1sos pr\u00f3ba, vagy sebgy\u00f3gyul\u00e1si pr\u00f3ba a biol\u00f3gi\u00e1ban \u00e1ltal\u00e1nosan haszn\u00e1lt pr\u00f3ba, mivel lehet\u0151v\u00e9 teszi az \u00e9lettani \u00e9s k\u00f3ros sejtes esem\u00e9nyek m\u00f6g\u00f6ttes mechanizmus\u00e1nak elemz\u00e9s\u00e9t. <\/p>\n\n\n\n<p>A sebgy\u00f3gyul\u00e1s tanulm\u00e1nyoz\u00e1sa az angiogenezis \u00e9s a tumorfejl\u0151d\u00e9s mellett a fejl\u0151d\u00e9s \u00e9s a sz\u00f6veti modellez\u00e9s meg\u00e9rt\u00e9s\u00e9nek fontos m\u00f3dja. <\/p>\n\n\n\n<p>Ha a vizsg\u00e1latot k\u00e9t dimenzi\u00f3ban v\u00e9gezz\u00fck, akkor m\u00e9rhet\u0151, hogy a sejtek milyen gyorsan reag\u00e1lnak a sebre, a meghat\u00e1rozott ter\u00fcletet lefedve.<\/p>\n\n\n\n<p>M\u00e1s szavakkal, a k\u00eds\u00e9rlet alapvet\u0151en az, hogy egy plakettben l\u00e9v\u0151 monor\u00e9tegben l\u00e9v\u0151 \u00f6sszefoly\u00f3 sejteken r\u00e9st hozzunk l\u00e9tre. <\/p>\n\n\n\n<p>Id\u0151vel a sejtek elkezdenek v\u00e1ndorolni, hogy kit\u00f6lts\u00e9k a r\u00e9st, \u00e9s a sejtek v\u00e1ndorl\u00e1si sebess\u00e9ge m\u00e9rhet\u0151. <\/p>\n\n\n\n<p>Ezut\u00e1n a sejtv\u00e1ndorl\u00e1si sebess\u00e9g m\u00e9r\u00e9s\u00e9hez k\u00e9peket kell k\u00e9sz\u00edteni\u00fck, m\u00e9ghozz\u00e1 egy csom\u00f3 k\u00e9pet, ami viszont az elemz\u00e9s probl\u00e9m\u00e1s l\u00e9p\u00e9se, mert k\u00e9zi m\u00e9r\u00e9st ig\u00e9nyel. <\/p>\n\n\n\n<p>Szerencs\u00e9re ma m\u00e1r rengeteg technol\u00f3gia \u00e1ll rendelkez\u00e9s\u00fcnkre, hogy jav\u00edtsuk \u00e9s korszer\u0171s\u00edts\u00fck az elemz\u00e9si cs\u0151vezet\u00e9k\u00fcnket, lehet\u0151v\u00e9 t\u00e9ve a tud\u00f3sok sz\u00e1m\u00e1ra, hogy jobb \u00e9s szem\u00e9lyre szabottabb m\u00f3dszereket alkalmazzanak az eredm\u00e9ny el\u00e9r\u00e9s\u00e9hez. <\/p>\n\n\n\n<p>Ahogy Garcia-Fossa \u00e9s Gaal tette.<\/p>\n\n\n\n<p>Ma m\u00e1r m\u00e1s kereskedelmi \u00e9s nem kereskedelmi eszk\u00f6z\u00f6k is rendelkez\u00e9sre \u00e1llnak a sebter\u00fclet feldolgoz\u00e1s\u00e1hoz. <\/p>\n\n\n\n<p>De ezek nem olyan egyszer\u0171ek, mint a PyScratch, \u00e9s a felhaszn\u00e1l\u00f3t\u00f3l bizonyos szint\u0171 programoz\u00e1st ig\u00e9nyelnek, \u00e9s a felhaszn\u00e1l\u00f3 teljes idej\u0171 figyelm\u00e9t is megk\u00f6vetelik, \u00edgy az elemz\u00e9s hajlamosabb az emberi hib\u00e1kra, valamint a kutat\u00f3nak az \u00f6sszes k\u00e9p \u00e9s adat elemz\u00e9s\u00e9re ford\u00edtott id\u0151re.<\/p>\n\n\n\n<p>A cikkben a szerz\u0151k elmagyar\u00e1zz\u00e1k a szoftver m\u0171k\u00f6d\u00e9s\u00e9t. A k\u00eds\u00e9rlet sor\u00e1n k\u00e9sz\u00edtett \u00f6sszes k\u00e9pb\u0151l a felhaszn\u00e1l\u00f3 egy vessz\u0151vel elv\u00e1lasztott \u00e9rt\u00e9kek (.cvs) f\u00e1jlt kap, egy olyan kimenetet, amely t\u00e1bl\u00e1zatos adatokat t\u00e1rol egyszer\u0171 sz\u00f6vegben. <\/p>\n\n\n\n<p>A felhaszn\u00e1l\u00f3 ezut\u00e1n a szok\u00e1sos rutin szerint feldolgozhatja az adatokat. Garcia-Fossa szerint a program elengedhetetlen volt a mesterszakos diplomamunk\u00e1j\u00e1hoz: \"A szoftver a bemeneti adatokat olyan \u00e9rt\u00e9kk\u00e9 alak\u00edtja \u00e1t, amelyeknek biol\u00f3giai \u00e9rtelme van, mint p\u00e9ld\u00e1ul a sejtv\u00e1ndorl\u00e1s sebess\u00e9ge. <\/p>\n\n\n\n<p>A PyScratch-nak k\u00f6sz\u00f6nhet\u0151en jobban tudtam elemezni a nanor\u00e9szecsk\u00e9m hat\u00e1s\u00e1t a prosztatar\u00e1kos sejtekre, \u00e9s meg tudtam m\u00e9rni a sejtv\u00e1ndorl\u00e1s sebess\u00e9g\u00e9t, valamint a j\u00f3 z\u00e1r\u00f3d\u00e1s pontos idej\u00e9t.\"<\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter size-large\"><a href=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/01\/process.png\"><img decoding=\"async\" loading=\"lazy\" width=\"1024\" height=\"409\" src=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/01\/process-1024x409.png\" alt=\"\" class=\"wp-image-12132\" srcset=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/01\/process-1024x409.png 1024w, https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/01\/process-300x120.png 300w, https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/01\/process-768x307.png 768w, https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/01\/process-1536x614.png 1536w, https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/01\/process.png 1563w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/a><\/figure><\/div>\n\n\n\n<p>Ha ki szeretn\u00e9 pr\u00f3b\u00e1lni a PyScratch-ot a kutat\u00e1saihoz, a szoftver szabadon hozz\u00e1f\u00e9rhet\u0151, \u00e9s a tudom\u00e1nyos k\u00f6z\u00f6ss\u00e9g minden tagja haszn\u00e1lhatja, seg\u00edtve Garcia-Fossa \u00e9s Gaal munk\u00e1j\u00e1t a program tov\u00e1bbfejleszt\u00e9s\u00e9ben \u00e9s t\u00f6k\u00e9letes\u00edt\u00e9s\u00e9ben.<\/p>\n\n\n\n<p>&nbsp;____<\/p>\n\n\n\n<p>Milyenek az eddigi Mind the Graph tapasztalataid? Seg\u00edtsen nek\u00fcnk, hogy jav\u00edthassuk platformunkat az \u00d6n \u00e9s sok m\u00e1s tud\u00f3s sz\u00e1m\u00e1ra az Mind the Graph \u00e9rt\u00e9kel\u00e9s\u00e9nek meg\u00edr\u00e1s\u00e1val. Mes\u00e9ljen nek\u00fcnk a tapasztalatair\u00f3l, csak kattintson<a href=\"https:\/\/www.saashub.com\/mind-the-graph-reviews\/new\"> itt<\/a>.&nbsp;<\/p>","protected":false},"excerpt":{"rendered":"<p>A PyScratch egy \u00faj, ny\u00edlt forr\u00e1sk\u00f3d\u00fa, Python nyelven implement\u00e1lt szoftver a migr\u00e1ci\u00f3s adatok elemz\u00e9s\u00e9hez, amely felhaszn\u00e1l\u00f3bar\u00e1t fel\u00fclettel rendelkezik, amely lehet\u0151v\u00e9 teszi a kev\u00e9s vagy semmilyen programoz\u00e1si ismerettel nem rendelkez\u0151 tud\u00f3sok sz\u00e1m\u00e1ra a haszn\u00e1lat\u00e1t. A szoftvert a Campinasi Egyetem NanoCell Interactions laborat\u00f3rium\u00e1nak h\u00e1rom brazil tud\u00f3s\u00e1nak egy\u00fcttm\u0171k\u00f6d\u00e9s\u00e9ben tervezt\u00e9k 2017-ben. Ez volt [...]<\/p>","protected":false},"author":4,"featured_media":12133,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[959,28],"tags":[817,554,964],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v19.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>A new tool for processing spatial and temporal information<\/title>\n<meta name=\"description\" content=\"Read this blog to know what PyScratch is and how that new tool processes spatial and temporal information from scratch assays.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mindthegraph.com\/blog\/hu\/saggi-pyscratch-spatial-and-temporal-information-from-scratch\/\" \/>\n<meta property=\"og:locale\" content=\"hu_HU\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"A 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