{"id":11803,"date":"2020-10-28T09:30:35","date_gmt":"2020-10-28T11:30:35","guid":{"rendered":"https:\/\/mindthegraph.com\/blog\/?p=11803"},"modified":"2022-10-18T08:09:32","modified_gmt":"2022-10-18T11:09:32","slug":"rt-pcr-test-for-coronavirus","status":"publish","type":"post","link":"https:\/\/mindthegraph.com\/blog\/hu\/rt-pcr-teszt-a-koronavirushoz\/","title":{"rendered":"A koronav\u00edrus RT-PCR tesztje"},"content":{"rendered":"

A 2020-ban a COVID-19-r\u0151l hallott sok mindenb\u0151l egy dolog van a k\u00f6z\u00e9ppontban: az a teszt, amellyel kimutathat\u00f3, hogy valaki fert\u0151z\u00f6tt-e a v\u00edrussal, a h\u00edres RT-PCR teszt vagy csak PCR.<\/span><\/p>\n

Bizony\u00e1ra hallott m\u00e1r err\u0151l a vizsg\u00e1latr\u00f3l, ak\u00e1r egy eg\u00e9szs\u00e9g\u00fcgyi szakembert\u0151l, ak\u00e1r a h\u00edrekb\u0151l. A PCR-tesztet a v\u00edrus jelenl\u00e9t\u00e9nek (vagy nem jelenl\u00e9t\u00e9nek) kimutat\u00e1s\u00e1ra alkalmazz\u00e1k valakinek a szervezet\u00e9ben.<\/span><\/p>\n

Az RT-PCR teszt nem az egyetlen, amely k\u00e9pes kimutatni a v\u00edrust, de a t\u00f6bbi m\u00f3dszerrel \u00f6sszehasonl\u00edtva ez a legpontosabb \u00e9s legmegb\u00edzhat\u00f3bb. Egy m\u00e1sik vizsg\u00e1lat a hagyom\u00e1nyos szerol\u00f3giai vizsg\u00e1lat, amely a v\u00e9rmint\u00e1ban kimutathat\u00f3 specifikus ellenanyagszintet mutat ki.<\/span><\/p>\n

A bet\u0171k az RT-PCR-ben a k\u00f6vetkez\u0151ket jelentik <\/span>Ford\u00edtott transzkripci\u00f3s polimer\u00e1z l\u00e1ncreakci\u00f3<\/b>, a tud\u00f3sok \u00e9vtizedek \u00f3ta haszn\u00e1lj\u00e1k a genetikai anyag t\u00f6bbsz\u00f6r\u00f6s m\u00e1solat\u00e1nak el\u0151\u00e1ll\u00edt\u00e1s\u00e1ra.<\/span><\/p>\n

M\u00e1s sz\u00f3val, ebben az esetben t\u00f6bb genetikai anyagot a SARS-CoV-2-b\u0151l, amelyet ujjlenyomatk\u00e9nt haszn\u00e1lnak a v\u00edrus jelenl\u00e9t\u00e9nek igazol\u00e1s\u00e1ra az orrb\u00f3l, f\u00fclb\u0151l vagy a betegekt\u0151l gy\u0171jt\u00f6tt ny\u00e1lb\u00f3l sz\u00e1rmaz\u00f3 mint\u00e1kban. Ha a mint\u00e1ban ak\u00e1r kis mennyis\u00e9gben is v\u00edrus rejt\u0151zik, a m\u00e1sol\u00f3paszt\u00e1s ciklusa miatt k\u00e9pes lesz kimutatni. Ez\u00e9rt olyan megb\u00edzhat\u00f3 az RT-PCR teszt.<\/span><\/p>\n

A teszt<\/b> egy olyan m\u00f3dszer, amely alapvet\u0151en k\u00e9t l\u00e9p\u00e9sb\u0151l \u00e1ll a h\u0151m\u00e9rs\u00e9kleti cikluson bel\u00fcl, \u00e9s amely a genetikai anyag egy darabj\u00e1nak - ak\u00e1r DNS-nek, ak\u00e1r RNS-nek - a m\u00e1sol\u00e1si \u00e9s beilleszt\u00e9si folyamat\u00e1t v\u00e9gzi.<\/span><\/p>\n

A teszthez egy <\/span>termocikl\u00e1l\u00f3 g\u00e9p<\/b> amely pontos <\/span>h\u0151m\u00e9rs\u00e9klet- \u00e9s id\u0151szab\u00e1lyoz\u00e1s<\/b>. A t\u00f6bbsz\u00f6r\u00f6s h\u0151m\u00e9rs\u00e9kleti ciklusokon kereszt\u00fcl a v\u00edrus genetikai anyaga n\u00f6vekszik. A k\u00f6vetkez\u0151kben egy p\u00e9lda l\u00e1that\u00f3 egy h\u0151m\u00e9rs\u00e9kleti ciklusra.<\/span><\/p>\n

\"\"<\/a><\/p>\n

A m\u00f3dszerhez k\u00e9t f\u0151 enzimre van sz\u00fcks\u00e9g, a ford\u00edtott transzkript\u00e1zra (ez\u00e9rt az \"RT\" a c\u00edmben) \u00e9s a DNS-polimer\u00e1zra. Mindegyiknek van egy-egy hat\u00e1sf\u00e1zisa \u00e9s funkci\u00f3ja. A fenti \u00e1br\u00e1n a ciklus s\u00e1rga f\u00e1zisa az, amikor az RT hat, a piros f\u00e1zis pedig az, amikor a DNS-polimer\u00e1z v\u00e9gzi a dolg\u00e1t.<\/span><\/p>\n

Az els\u0151 enzim elk\u00e9sz\u00edti a komplementer DNS-sz\u00e1lat az RNS-v\u00edrusb\u00f3l, lehet\u0151v\u00e9 t\u00e9ve a m\u00e1sodik enzim sz\u00e1m\u00e1ra, hogy elv\u00e9gezze a m\u00e1sol\u00e1s-beilleszt\u00e9s munk\u00e1j\u00e1t az \u00e9ppen elk\u00e9sz\u00edtett \u00faj DNS-sz\u00e1lb\u00f3l, n\u00f6velve a jelen l\u00e9v\u0151 genetikai anyag mennyis\u00e9g\u00e9t. Ez az amplifik\u00e1ci\u00f3 lehet\u0151v\u00e9 teszi a genetikai anyag jellemz\u00e9s\u00e9t \u00e9s\/vagy manipul\u00e1l\u00e1s\u00e1t, amikor kisebb mennyis\u00e9gben ez nem lenne lehets\u00e9ges.<\/span><\/p>\n

\"\"<\/a><\/p>\n

Mindehhez az enzimeknek egy PRIMER nev\u0171 kis (vagy nagy) molekula seg\u00edts\u00e9g\u00e9re van sz\u00fcks\u00e9g\u00fck. A primer feladata, hogy jelezze azt a helyet, ahol az enzimnek el kell kezdenie a sz\u00e1lak meghosszabb\u00edt\u00e1s\u00e1t, en\u00e9lk\u00fcl nem indul el a DNS-polimer\u00e1z m\u00e1sol\u00e1si folyamata.<\/span><\/p>\n

Az alapoz\u00f3 v\u00e1laszthat\u00f3 v\u00e9letlenszer\u0171 vagy meghat\u00e1rozott alak\u00fa. Ilyen esetekben nagyon fontos ismerni a v\u00edrus genetikai t\u00e9rk\u00e9p\u00e9t, hogy specifikus primereket lehessen haszn\u00e1lni az eredm\u00e9nyek hat\u00e9konys\u00e1g\u00e1nak \u00e9s megb\u00edzhat\u00f3s\u00e1g\u00e1nak n\u00f6vel\u00e9se \u00e9rdek\u00e9ben.<\/span><\/p>\n

Ha hozz\u00e1nk hasonl\u00f3an k\u00edv\u00e1ncsi, \u00e9s szeretne t\u00f6bb r\u00e9szletet megtudni az RT-PCR-r\u0151l, akkor tov\u00e1bbi r\u00e9szleteket tudhat meg.\u00a0<\/span> ez a tudom\u00e1nyos cikk <\/span>itt<\/span><\/a>\u00a0 de l\u00e9nyeg\u00e9ben itt van minden, ami a teszt elv\u00e9gz\u00e9s\u00e9hez sz\u00fcks\u00e9ges:<\/span><\/p>\n

A v\u00edrus genetikai anyaga, az RNS<\/strong><\/span><\/p>\n

\"\"<\/a><\/p>\n

Ford\u00edtott transzkript\u00e1z \u00e9s DNS-polimer\u00e1z enzimek<\/b><\/span><\/p>\n

\"\"<\/a><\/p>\n

PRIMERS<\/b><\/span><\/p>\n

\"\"<\/a><\/p>\n

Nukleozid-trifoszf\u00e1tok<\/b><\/span><\/p>\n

\"\"<\/a><\/p>\n

\u00c9s fluoreszcens riporterfest\u00e9kek:\u00a0<\/b><\/span><\/p>\n

A COVID-19 v\u00edrussal fert\u0151z\u00f6tt vagy nem fert\u0151z\u00f6tt minta eredm\u00e9nyeinek megismer\u00e9s\u00e9hez a fluoreszcens riportfest\u00e9k vizsg\u00e1latot az RT-PCR teszttel kombin\u00e1lva haszn\u00e1lj\u00e1k. A vizsg\u00e1lat a minta fluoreszcens jel\u00e9nek jelenl\u00e9t\u00e9n vagy hi\u00e1ny\u00e1n alapul. Jel hi\u00e1ny\u00e1ban a teszt negat\u00edvnak vagy hamis negat\u00edvnak tekinthet\u0151. Intenz\u00edv jel jelenl\u00e9t\u00e9ben nemcsak a v\u00edrus jelenl\u00e9t\u00e9re lehet pozit\u00edvan k\u00f6vetkeztetni, hanem el\u0151rehaladott st\u00e1dium\u00fa betegs\u00e9gre is.<\/span><\/p>\n

Keverje \u00f6ssze az \u00f6sszes hozz\u00e1val\u00f3t, \u00e9s tegye be a termociklikus g\u00e9pbe.\u00a0<\/b><\/span><\/p>\n

A teszt els\u0151 l\u00e9p\u00e9se a k\u00f6vetkez\u0151 infografik\u00e1n l\u00e1that\u00f3. Az RT enzim elk\u00e9sz\u00edti a mint\u00e1ban l\u00e9v\u0151 v\u00edrus genetikai anyag\u00e1nak els\u0151 m\u00e1solat\u00e1t. Ezt k\u00f6vet\u0151en az RT-PCR m\u00f3dszer m\u00e1sodik l\u00e9p\u00e9se l\u00e1that\u00f3. A primer jelzi a kezd\u0151 poz\u00edci\u00f3t, \u00e9s a DNS-polimer\u00e1z enzimatikus reakci\u00f3 r\u00e9v\u00e9n elkezdi meghosszabb\u00edtani a DNS-sz\u00e1lakat.<\/span><\/p>\n

\"A<\/a><\/p>\n

\"\"<\/a><\/p>\n

\"\"<\/a><\/p>\n

V\u00e9g\u00fcl, ez a k\u00e9p a SARS-CoV-2 v\u00edrus elektronmikroszk\u00f3pos k\u00e9pe, \u00e9s t\u00f6k\u00e9letesen l\u00e1that\u00f3 a v\u00edrus szerkezete. H\u00e1t nem gy\u00f6ny\u00f6r\u0171?\u00a0\u00a0<\/span><\/p>\n

A teszt \u00f6sszetett \u00e9s nem olyan egyszer\u0171, mint amilyennek l\u00e1tszik, de fogadok, hogy az infografik\u00e1val sokkal k\u00f6nnyebb volt meg\u00e9rteni a nagy k\u00e9pet, hogyan m\u0171k\u00f6dik, igaz?<\/span><\/p>\n

Ezt b\u00e1rmilyen tudom\u00e1nyos inform\u00e1ci\u00f3val megteheti a k\u00f6vetkez\u0151 eszk\u00f6z\u00f6kkel\u00a0<\/span> Mind the Graph<\/span><\/a>. A platform az\u00e9rt van itt, hogy \u00f6n\u00e1ll\u00f3s\u00e1got adjon \u00f6nnek a tudom\u00e1nyos inform\u00e1ci\u00f3k jobb kommunik\u00e1l\u00e1s\u00e1hoz. Tudjon meg t\u00f6bbet a platform haszn\u00e1lat\u00e1r\u00f3l, \u00e9s ha seg\u00edts\u00e9gre van sz\u00fcks\u00e9ge, k\u00e9rj\u00fck, l\u00e9pjen kapcsolatba vel\u00fcnk.<\/span>!<\/b><\/p>\n

Hivatkoz\u00e1s<\/b><\/p>\n

AI, T. et al. A mellkas CT \u00e9s az RT-PCR vizsg\u00e1lat korrel\u00e1ci\u00f3ja a 2019-es koronav\u00edrus betegs\u00e9g (COVID-19) eset\u00e9ben K\u00edn\u00e1ban: A Report of 1014 Cases. Radiology, v. 296, n. 2, p. E32-E40, 26 fev. 2020.<\/span><\/p>\n

GIBELLINI, D. et al. Az 1-es t\u00edpus\u00fa hum\u00e1n immundeficiencia-v\u00edrus (HIV-1) v\u00edrusterhel\u00e9s mennyis\u00e9gi kimutat\u00e1sa SYBR z\u00f6ld val\u00f3s idej\u0171 RT-PCR technik\u00e1val HIV-1 szeropozit\u00edv betegekn\u00e9l. Journal of Virological Methods, v. 115, n. 2, p. 183-189, 2004. febr. 1.<\/span><\/p>\n

JAMES, D. Egyszer\u0171 \u00e9s megb\u00edzhat\u00f3 protokoll az almat\u00f6rzs bar\u00e1zd\u00e1l\u00f3d\u00e1si v\u00edrus\u00e1nak kimutat\u00e1s\u00e1ra RT-PCR \u00e9s multiplex PCR-m\u00f3dszerrel. Journal of Virological Methods, 83. \u00e9vf. 83. sz\u00e1m, 1-9. o., 1999. december 1.<\/span><\/p>\n

LI, Y. et al. A SARS-CoV-2 RT-PCR-vizsg\u00e1lat stabilit\u00e1si k\u00e9rd\u00e9sei k\u00f3rh\u00e1zi betegekn\u00e9l, akiket klinikailag COVID-19-vel diagnosztiz\u00e1ltak. Journal of Medical Virology, v. 92, n. 7, p. 903-908, 2020.<\/span><\/p>\n

LIAO, C.-S. et al. Miniat\u0171r RT-PCR rendszer az RNS-alap\u00fa v\u00edrusok diagnosztiz\u00e1l\u00e1s\u00e1ra. Nucleic Acids Research, v. 33, n. 18, p. e156-e156, 1 out. 2005.<\/span><\/p>\n

MOHAN, A. et al. A PCR \u00e9s RT-PCR seg\u00edts\u00e9g\u00e9vel kimutatott v\u00edrusfert\u0151z\u00e9s prevalenci\u00e1ja a COPD akut exacerb\u00e1ci\u00f3j\u00e1ban szenved\u0151 betegekn\u00e9l: szisztematikus \u00e1ttekint\u00e9s. Respirology, v. 15, n. 3, p. 536-542, 2010.<\/span><\/p>\n

NOLAN, T.; HANDS, R. E.; BUSTIN, S. A. Az mRNS mennyis\u00e9gi meghat\u00e1roz\u00e1sa val\u00f3s idej\u0171 RT-PCR seg\u00edts\u00e9g\u00e9vel. Nature Protocols, v. 1, n. 3, p. 1559-1582, ago. 2006.<\/span><\/p>\n

\u00daj koronav\u00edrus SARS-CoV-2 | Ez a transzmisszi\u00f3s elektron... | Flickr. Dispon\u00edvel em: . Acesso em: 23 jul. 2020. <\/span><\/p>","protected":false},"excerpt":{"rendered":"","protected":false},"author":4,"featured_media":11847,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[959,28],"tags":[388,799,554,798,250],"yoast_head":"\nThe RT-PCR Test for Coronavirus - Mind the Graph Blog<\/title>\n<meta name=\"description\" content=\"Read this blog to know everything related to the popular COVID-19 test, RT-PCR or only PCR. You will get in-depth information about this test of Coronavirus.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mindthegraph.com\/blog\/hu\/rt-pcr-test-for-coronavirus\/\" \/>\n<meta property=\"og:locale\" content=\"hu_HU\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"The RT-PCR Test for Coronavirus - Mind the Graph Blog\" \/>\n<meta property=\"og:description\" content=\"Read this blog to know everything related to the popular COVID-19 test, RT-PCR or only PCR. 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He has a Ph.D. and solid scientific background in Psychopharmacology and experience as a Guest Researcher at the Max Planck Institute of Psychiatry (Germany) and Researcher in D'Or Institute for Research and Education (IDOR, Brazil). Fabricio holds over 2500 citations in Google Scholar. He has 10 years of experience in small innovative businesses, with relevant experience in product design and innovation management. Connect with him on LinkedIn - Fabricio Pamplona.","sameAs":["http:\/\/mindthegraph.com","https:\/\/www.linkedin.com\/in\/fabriciopamplona"],"url":"https:\/\/mindthegraph.com\/blog\/hu\/author\/fabricio\/"}]}},"_links":{"self":[{"href":"https:\/\/mindthegraph.com\/blog\/hu\/wp-json\/wp\/v2\/posts\/11803"}],"collection":[{"href":"https:\/\/mindthegraph.com\/blog\/hu\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/mindthegraph.com\/blog\/hu\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/mindthegraph.com\/blog\/hu\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/mindthegraph.com\/blog\/hu\/wp-json\/wp\/v2\/comments?post=11803"}],"version-history":[{"count":3,"href":"https:\/\/mindthegraph.com\/blog\/hu\/wp-json\/wp\/v2\/posts\/11803\/revisions"}],"predecessor-version":[{"id":11850,"href":"https:\/\/mindthegraph.com\/blog\/hu\/wp-json\/wp\/v2\/posts\/11803\/revisions\/11850"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/mindthegraph.com\/blog\/hu\/wp-json\/wp\/v2\/media\/11847"}],"wp:attachment":[{"href":"https:\/\/mindthegraph.com\/blog\/hu\/wp-json\/wp\/v2\/media?parent=11803"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/mindthegraph.com\/blog\/hu\/wp-json\/wp\/v2\/categories?post=11803"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/mindthegraph.com\/blog\/hu\/wp-json\/wp\/v2\/tags?post=11803"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}