{"id":16636,"date":"2022-09-10T18:27:00","date_gmt":"2022-09-10T21:27:00","guid":{"rendered":"https:\/\/mindthegraph.com\/blog\/?p=16636"},"modified":"2023-03-17T14:47:45","modified_gmt":"2023-03-17T17:47:45","slug":"success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids","status":"publish","type":"post","link":"https:\/\/mindthegraph.com\/blog\/fi\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/","title":{"rendered":"FGF-signaalinanto paksusuolen sy\u00f6v\u00e4n organoidien itseuudistumisessa Paperin katsaus"},"content":{"rendered":"

FGFR:n eston aiheuttama vammainen sferoidien ja organoidien muodostuminen potilasper\u00e4isiss\u00e4 CRC-soluissa on paljon helpompi ymm\u00e4rt\u00e4\u00e4 Mind the Graph:n kuvien avulla. <\/p>\n\n\n\n

Kirjoittajan laatimasta kaavasta k\u00e4y ilmi, ett\u00e4 kirurgiset koepalat pilkottiin entsymaattisesti, jotta saatiin yksitt\u00e4isten solujen suspensio. Naiiveja lajittelemattomia soluja viljeltiin FGF2:ta sis\u00e4lt\u00e4v\u00e4ss\u00e4 CSC-mediumissa tai FGFR-inhibiittorilla SU-5402.<\/p>\n\n\n\n

Fibroblastikasvutekij\u00e4 2:n (FGF2) roolia kolorektaalisy\u00f6p\u00e4kantasolujen uudistumisessa on tutkittu.<\/strong><\/h2>\n\n\n\n

J\u00f6rg Otte ja kollegat (2019) raportoivat artikkelissaan \"FGF Signalling in the Self-Renewal of Colon Cancer Organoids\", ett\u00e4 kun naiivit yksitt\u00e4iset solut upotettiin Matrigeliin ja niit\u00e4 kasvatettiin CSC-mediassa, havaittiin monimutkaisempaa itseorganisoitumista kaikilla analysoiduilla potilailla. <\/p>\n\n\n\n

Geenit, jotka liittyv\u00e4t suoraan EMT:hen sek\u00e4 mesenkymaaliseen ja itseuudistuvaan fenotyyppiin (TGFB3, ID1, ID2 ja ID4), havaittiin yliekspressoituneiksi organoideissa. <\/p>\n\n\n\n

Vaikka FGFR:n esto johti tehostuneeseen MAPK-signalointiin, he havaitsivat indusoituneen erilaistumisen, useimpien kantasolumerkkien h\u00e4vi\u00e4misen ja epiteelin fenotyypin. <\/p>\n\n\n\n

Sy\u00f6p\u00e4kantasolut ovat pahanlaatuisten solujen osapopulaatio, joka kykenee uusiutumaan itse ja toimimaan jatkuvana l\u00e4hteen\u00e4 erilaistuneille kasvainsoluille. Joissakin CSC-viljelyprotokollissa fibroblastikasvutekij\u00e4 2 on t\u00e4rke\u00e4 ainesosa kantasolujen s\u00e4ilymisen kannalta. <\/p>\n\n\n\n

CSC-v\u00e4liaineessa viljellyiss\u00e4 organoideissa havaittiin sen sijaan TGF-\u03b2:n ja kaikkien nelj\u00e4n erilaistumisen est\u00e4j\u00e4geeniperheen j\u00e4senen induktio.<\/p>\n\n\n\n

Tutkimukseen osallistui 9 per\u00e4kk\u00e4ist\u00e4 valitsematonta CRC-potilasta.<\/strong><\/p>\n\n\n\n

Tarkista alkuper\u00e4inen artikkeli t\u00e4\u00e4ll\u00e4<\/a>.<\/p>\n\n\n\n

Graafinen abstrakti, jonka taustalla on Mind the Graph<\/strong><\/h2>\n\n\n\n
<\/div>\n\n\n
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Valmistettu Mind the Graph<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

Jotkin heid\u00e4n johtop\u00e4\u00e4t\u00f6ksist\u00e4\u00e4n vahvistavat mahdollisesti sit\u00e4, mit\u00e4 alasta on aiemmin tiedetty: \"Pluripotenssiin liittyv\u00e4t geenit NANOG, LIN28 ja SALL1 ilmentyiv\u00e4t yksinomaan hESC:ss\u00e4. GO-termit \"BMP-signalointireitin negatiivinen s\u00e4\u00e4tely\" ja \"SMAD-proteiinien signaalinsiirto\" havaittiin hESC-kulttuurissamme\", Otte ehdotti.<\/p>\n\n\n\n

Kirjoittajat my\u00f6nt\u00e4v\u00e4t, ett\u00e4 \"GO-termin analyysi t\u00e4st\u00e4 geenijoukosta tuotti vain muutamia merkitt\u00e4vi\u00e4 tuloksia johtuen yleisesti s\u00e4\u00e4nneltyjen geenien v\u00e4h\u00e4isest\u00e4 m\u00e4\u00e4r\u00e4st\u00e4. Useimmat n\u00e4ist\u00e4 GO-termeist\u00e4 CSC-medium-olosuhteissa liittyiv\u00e4t aktiiviseen transkriptioon, solujen lis\u00e4\u00e4ntymiseen tai lis\u00e4\u00e4ntyneeseen glukoosinottoon. <\/p>\n\n\n\n

FGFR:n eston j\u00e4lkeen l\u00f6ysimme solusyklin s\u00e4\u00e4telij\u00f6it\u00e4 sek\u00e4 monia geenej\u00e4, jotka on annotoitu \"epiteelisolujen erilaistumisen\", \"ruoansulatuksen\" tai \"sappihappojen ja -suolojen kuljetuksen\" kanssa.\"\"<\/p>\n\n\n\n

Paperit Vaikutus ja suorituskyky<\/strong><\/h2>\n\n\n\n

Voimme analysoida Altmetric<\/a> tiedot, joiden mukaan t\u00e4m\u00e4n asiakirjan verkkohuomion pistem\u00e4\u00e4r\u00e4 on korkeampi... kuin 68%<\/strong><\/strong> kaikista muista samanik\u00e4isist\u00e4 Scientific Reportsin artikkeleista.<\/strong><\/p>\n\n\n\n

<\/div>\n\n\n
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L\u00e4hde Altmetrics<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

Paranna artikkelisi saavutettavuutta my\u00f6s infografiikan avulla.<\/p>\n\n\n\n

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Kokeile Mind the Graph<\/strong><\/a><\/div>\n<\/div>\n\n\n\n
<\/div>","protected":false},"excerpt":{"rendered":"

FGFR:n eston aiheuttama vammaisten sferoidien ja organoidien muodostuminen potilasper\u00e4isiss\u00e4 CRC-soluissa on helpompi ymm\u00e4rt\u00e4\u00e4 kuviemme avulla.<\/p>","protected":false},"author":29,"featured_media":20014,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[195,958,957,1],"tags":[96,554,775,963],"yoast_head":"\nFGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog<\/title>\n<meta name=\"description\" content=\"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mindthegraph.com\/blog\/fi\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/\" \/>\n<meta property=\"og:locale\" content=\"fi_FI\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - 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