{"id":8728,"date":"2019-04-23T16:35:01","date_gmt":"2019-04-23T19:35:01","guid":{"rendered":"https:\/\/blog.mindthegraph.com\/?p=8728"},"modified":"2022-10-18T08:23:59","modified_gmt":"2022-10-18T11:23:59","slug":"visual-abstract-example","status":"publish","type":"post","link":"https:\/\/mindthegraph.com\/blog\/et\/visual-abstract-example\/","title":{"rendered":"Kasutaja poolt loodud visuaalne kokkuv\u00f5te, mis selgitab Yelena Sterlini uurimust\u00f6\u00f6d \"Optimized Small-Molecule Pull-Downs Define MLBP1 as an Acyl-Lipid-Binding Protein\"."},"content":{"rendered":"

Visuaalset abstraktsiooni ei ole lihtne luua, kuid see on siin, et j\u00e4\u00e4da. Nii et meile meeldib alati n\u00e4idata meie kasutajate h\u00e4mmastavaid t\u00f6id visuaalse abstraktsiooni inspireeriva n\u00e4itena. Me tahame anda teadlastele v\u00f5imaluse muuta teadus visuaalsemaks ja k\u00e4ttesaadavamaks.<\/p>\n

Postituses n\u00e4itan teile natuke algset uurimist\u00f6\u00f6d, mille on teinud Jelena Sterlin<\/a> ja kolleegid. Ta on doktorant Jeruusalemma heebrea \u00fclikooli p\u00f5llumajanduse, toidu ja keskkonna teaduskonnas.<\/p>\n

Tema uus raamat Optimeeritud v\u00e4ikemolekulide t\u00f5mbedistsipliinid m\u00e4\u00e4ratlevad MLBP1 ats\u00fc\u00fcl-lipiidi siduvate valkudega.<\/a> <\/strong>valgustab valgude ja metaboliitide vastastikm\u00f5ju taimedes, keskendudes abstsiinhappe (ABA) retseptoritele. Ta on ka mind the graph'i tellija, nii et ta l\u00f5i infograafika sisse vaata graafikut<\/a> selgitada oma metoodikat.<\/p>\n

START-domeeniga seotud valkude t\u00e4htsus<\/strong><\/h2>\n

 <\/p>\n

Vastavalt Looduse ajakiri<\/a>, abscisiinhape (ABA) on peamine f\u00fctohormoon, mis osaleb abiootilisele stressile reageerimisel ja sellega kohanemisel. Samuti on see p\u00f5llumajanduskemikaalide kandidaat. ABA avastati esmakordselt 1960ndatel aastatel. P\u00e4rast seda on leitud, et see on peamine regulaator sellistes erinevates protsessides nagu dormantsus, idanemine, seemnete areng ning reageerimine biootilisele ja abiootilisele stressile.<\/p>\n

Varaseimad s\u00fcndmused, mis h\u00f5lmavad ABA signaalirada, h\u00f5lmavad ABA seondumist PYR\/PYL\/RCARs retseptoritega. Retseptorid kuuluvad START-domeeni superperekonda.<\/p>\n

START-valgu domeenid on lipiide siduv domeen, mis esineb bakterite, algloomade, p\u00e4rmi, taimede ja loomade genoomides. Siiski on need sagedamini levinud taimedes. Vastavalt Schrick<\/a>START-domeenide esinemine evolutsiooniliselt kaugel asuvatel liikidel, nagu loomad ja taimed, viitab konserveerunud mehhanismile, mis v\u00f5imaldab valkude ja lipiidide\/steroolide vahelist suhtlemist.<\/p>\n

V\u00e4ljaandes The Plant Journal<\/a> keskendub PYR\/PYL\/RCARs ABA retseptoritele. Teadlased otsustasid optimeerida meetodit, mis v\u00f5imaldaks identifitseerida START-domeeni ja START-domeeniga seotud valkude ligandid taimedes, sest \"START-domeeni valkude ligandid on h\u00e4davajalikud nende valkude n\u00f5uetekohaseks toimimiseks nii loomadel kui ka taimedel ning on n\u00e4idatud, et taimsed START-domeeni valgud on seotud oluliste f\u00fcsioloogiliste protsessidega\".<\/em><\/p>\n

Visuaalne kokkuv\u00f5te metoodikast<\/strong><\/h2>\n

 <\/p>\n

Oma metoodika selgitamiseks l\u00f5id teadlased infograafika, millel on graafilised illustratsioonid. See on pilkup\u00fc\u00fcdev ja lihtsamini m\u00f5istetav. <\/strong><\/h4>\n

 <\/p>\n

Allpool olev infograafika n\u00e4itab eksperimentaalset t\u00f6\u00f6korraldust. Nagu n\u00e4ha, ekspresseeriti ja puhastati neli START-valku. Nad filtreerisid ja inkubeerisid supernatanti, mis sisaldas natiivset lahustuvat fraktsiooni, kasutades streptavidiinsefaroosi, mis on afiinsusvaikuse vaik usaldusv\u00e4\u00e4rse ja k\u00f5rge kontsentratsiooniga-lahutusega biomolekulide puhastamine. Seej\u00e4rel anal\u00fc\u00fcsisid nad alikvoote Western blot'i abil. Ligandi identifitseerimiseks elueerisid nad oletatavad ligandid ja tegid neile LC -MS (vedelikkromatograafia ja massispektromeetria) s\u00fcsteemianal\u00fc\u00fcsi. Huvipakkuvate putatiivsete ligandide molekulaarstruktuurid m\u00e4\u00e4rati tandem-massispektromeetria abil.<\/p>\n

 <\/p>\n

\"visuaalne<\/p>\n

Autorid esitasid hea tehnika valguliigandite esialgseks sondeerimiseks taimedes. Nad kohandasid l\u00e4henemisviisi START-ligandide avastamiseks. Siiski saab meetodit kohandada erineva keemilise iseloomuga ligandidele, kohandades anal\u00fc\u00fcsitingimusi.<\/p>\n

Kui soovite oma uurimist\u00f6\u00f6d n\u00e4idata teaduslike illustratsioonide ja visuaalsete kokkuv\u00f5tete abil, v\u00f5ite kasutada ka Mind the Graph. Niisiis, kas olete valmis parandama oma teaduskommunikatsiooni?<\/p>\n

\"eelvaade_250\"<\/a><\/p>","protected":false},"excerpt":{"rendered":"

Visuaalset abstraktsiooni ei ole lihtne luua, kuid see on siin, et j\u00e4\u00e4da. Nii et meile meeldib alati n\u00e4idata meie kasutajate h\u00e4mmastavaid t\u00f6id visuaalse abstraktsiooni inspireeriva n\u00e4itena. Me tahame anda teadlastele v\u00f5imaluse muuta teadus visuaalsemaks ja k\u00e4ttesaadavamaks. Selles postituses n\u00e4itan teile natuke [...]<\/p>","protected":false},"author":11,"featured_media":8741,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"image","meta":[],"categories":[193,40],"tags":[291,96,292,245,294,295,554,775,293,382],"yoast_head":"\nVisual abstract created by Mind the Graph user. Attractive scientific figure!<\/title>\n<meta name=\"description\" content=\"See the visual abstract created by the MindtheGraph subscriber Yelena Sterling to explain her research in an attractive and instructive way. 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