{"id":16636,"date":"2022-09-10T18:27:00","date_gmt":"2022-09-10T21:27:00","guid":{"rendered":"https:\/\/mindthegraph.com\/blog\/?p=16636"},"modified":"2023-03-17T14:47:45","modified_gmt":"2023-03-17T17:47:45","slug":"success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids","status":"publish","type":"post","link":"https:\/\/mindthegraph.com\/blog\/et\/success-case-fgf-signalisatsioon-koloonkrebi-organoidide-eneseuuendamisel\/","title":{"rendered":"FGF-signalisatsioon j\u00e4mesoolev\u00e4hi organoidide iseeneslikul uuenemisel Paberi \u00fclevaade"},"content":{"rendered":"

FGFR-i inhibeerimise t\u00f5ttu puudulikku sferoidi ja organoidi moodustumist patsiendist p\u00e4rinevates CRC-rakkudes on Mind the Graph illustratsioonide abil palju lihtsam m\u00f5ista. <\/p>\n\n\n\n

Autori koostatud skeem n\u00e4itab, et kirurgilised biopsiad lagundati ens\u00fcmaatiliselt, et saada \u00fcksikute rakkude suspensioon. Naiivseid sorteerimata rakke kultiveeriti FGF2 sisaldavas CSC-meediumis v\u00f5i FGFR-inhibiitoriga SU-5402.<\/p>\n\n\n\n

Uuritud on fibroblastide kasvufaktori 2 (FGF2) rolli kolorektaalse v\u00e4hi t\u00fcvirakkude iseeneslikul uuenemisel.<\/strong><\/h2>\n\n\n\n

J\u00f6rg Otte ja kolleegid (2019) teatasid uurimuses \"FGF-signalisatsioon j\u00e4mesoolev\u00e4hi organoidide iseeneslikus uuenemises\", et kui naiivseid \u00fcksikuid rakke manustati Matrigelisse ja kasvatati CSC-vahendis, t\u00e4heldati k\u00f5igil anal\u00fc\u00fcsitud patsientidel keerukamat iseeneslikku organiseerumist. <\/p>\n\n\n\n

Leiti, et EMT-ga otseselt seotud geenid koos mesenh\u00fc\u00fcmilise ja iseenesliku fenot\u00fc\u00fcbiga (TGFB3, ID1, ID2 ja ID4) olid organoidides \u00fcleekspresseeritud. <\/p>\n\n\n\n

Kuigi FGFR-i inhibeerimine p\u00f5hjustas MAPK-signalisatsiooni suurenemise, t\u00e4heldasid nad indutseeritud diferentseerumist, enamiku t\u00fcvirakkude markerite kadumist ja epiteliaalse fenot\u00fc\u00fcbi teket. <\/p>\n\n\n\n

V\u00e4hi t\u00fcvirakud on pahaloomuliste rakkude alampopulatsioon, mis on v\u00f5imeline end ise taastama ja olema diferentseeritud kasvajarakkude pidevaks allikaks. M\u00f5nes CSC-kultuuri protokollis on fibroblastide kasvufaktor 2 oluline koostisosa t\u00fcvirakkude s\u00e4ilitamiseks. <\/p>\n\n\n\n

Seevastu CSC-vahenditega kultiveeritud organoidides tuvastasid nad TGF-\u03b2 ja k\u00f5igi nelja diferentseerumisinhibiitorite perekonna liikme induktsiooni.<\/p>\n\n\n\n

Uuringus osales 9 j\u00e4rjestikust valimata CRC-patsienti.<\/strong><\/p>\n\n\n\n

Kontrollige originaalartikkel siin<\/a>.<\/p>\n\n\n\n

Graafiline kokkuv\u00f5te, mille aluseks on Mind the Graph<\/strong><\/h2>\n\n\n\n
<\/div>\n\n\n
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Valmistatud koos Mind the Graph<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

M\u00f5ned nende j\u00e4reldused tugevdavad potentsiaalselt seda, mis oli varem selle valdkonna kohta teada: \"Pluripotentsusega seotud geene NANOG, LIN28 ja SALL1 v\u00e4ljendasid eranditult hESC-d. Meie hESC-kultuuris tuvastati GO-terminid \"BMP signaalitee negatiivne regulatsioon\" ja \"SMAD-valgu signaali\u00fclekanne\",\" pakkus Otte v\u00e4lja.<\/p>\n\n\n\n

Autorid tunnistavad, et \"selle geenikomplekti GO-terminoloogiline anal\u00fc\u00fcs andis vaid m\u00f5ned olulised tulemused, mis on tingitud \u00fchiselt reguleeritud geenide v\u00e4hesest arvust. Enamik neist GO-termidest CSC-mediumi seisundi puhul olid seotud aktiivse transkriptsiooni, rakkude proliferatsiooni v\u00f5i suurenenud gl\u00fckoosi tarbimisega. <\/p>\n\n\n\n

P\u00e4rast FGFR-i inhibeerimist leidsime rakuts\u00fckli regulaatoreid, samuti mitmeid geene, mis on annoteeritud \"epiteelirakkude diferentseerumise\", \"seedimise\" v\u00f5i \"sapphapete ja sapisoolade transpordiga\".\"<\/p>\n\n\n\n

Paberi m\u00f5ju ja tulemuslikkus<\/strong><\/h2>\n\n\n\n

Me saame anal\u00fc\u00fcsida koos Altmetric<\/a> andmed, et selle paberi online t\u00e4helepanu skoor on k\u00f5rgem kui 68%<\/strong><\/strong> k\u00f5ikidest teistest sarnase vanusega artiklitest Scientific Reportsis.<\/strong><\/p>\n\n\n\n

<\/div>\n\n\n
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Allikas Altmetrika<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

Parandage oma artikli k\u00e4ttesaadavust ka infograafiate abil.<\/p>\n\n\n\n

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Proovige Mind the Graph<\/strong><\/a><\/div>\n<\/div>\n\n\n\n
<\/div>","protected":false},"excerpt":{"rendered":"

FGFR-i inhibeerimise t\u00f5ttu puudulikku sferoidi ja organoidi moodustumist patsiendist p\u00e4rinevates CRC-rakkudes on meie illustratsioonide abil lihtsam m\u00f5ista.<\/p>","protected":false},"author":29,"featured_media":20014,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[195,958,957,1],"tags":[96,554,775,963],"yoast_head":"\nFGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog<\/title>\n<meta name=\"description\" content=\"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mindthegraph.com\/blog\/et\/success-case-fgf-signalisatsioon-koloonkrebi-organoidide-eneseuuendamisel\/\" \/>\n<meta property=\"og:locale\" content=\"et_EE\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog\" \/>\n<meta property=\"og:description\" content=\"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/mindthegraph.com\/blog\/et\/success-case-fgf-signalisatsioon-koloonkrebi-organoidide-eneseuuendamisel\/\" \/>\n<meta property=\"og:site_name\" content=\"Mind the Graph Blog\" \/>\n<meta property=\"article:published_time\" content=\"2022-09-10T21:27:00+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2023-03-17T17:47:45+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2022\/09\/blog_4.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1125\" \/>\n\t<meta property=\"og:image:height\" content=\"614\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Artur Zignani\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:image\" content=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2022\/09\/blog_4.jpg\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Artur Zignani\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"3 minutes\" \/>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - 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