{"id":12979,"date":"2021-06-08T20:10:05","date_gmt":"2021-06-08T23:10:05","guid":{"rendered":"https:\/\/mindthegraph.com\/blog\/?p=12979"},"modified":"2023-01-05T14:22:32","modified_gmt":"2023-01-05T17:22:32","slug":"microscopy-how-scientists-use-fluorescence-to-see-inside-cells","status":"publish","type":"post","link":"https:\/\/mindthegraph.com\/blog\/el\/microscopy-how-scientists-use-fluorescence-to-see-inside-cells\/","title":{"rendered":"\u039c\u03b9\u03ba\u03c1\u03bf\u03c3\u03ba\u03bf\u03c0\u03af\u03b1: \u03a0\u03ce\u03c2 \u03bf\u03b9 \u03b5\u03c0\u03b9\u03c3\u03c4\u03ae\u03bc\u03bf\u03bd\u03b5\u03c2 \u03c7\u03c1\u03b7\u03c3\u03b9\u03bc\u03bf\u03c0\u03bf\u03b9\u03bf\u03cd\u03bd \u03c6\u03b8\u03bf\u03c1\u03b9\u03c3\u03bc\u03cc \u03b3\u03b9\u03b1 \u03bd\u03b1 \u03b4\u03bf\u03c5\u03bd \u03bc\u03ad\u03c3\u03b1 \u03c3\u03c4\u03b1 \u03ba\u03cd\u03c4\u03c4\u03b1\u03c1\u03b1"},"content":{"rendered":"<p>\u0388\u03bd\u03b1 \u03bc\u03b5\u03b3\u03ac\u03bb\u03bf \u03bc\u03ad\u03c1\u03bf\u03c2 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\u03b5\u03c0\u03b9\u03c3\u03c4\u03ae\u03bc\u03bf\u03bd\u03b5\u03c2, \u03b5\u03bd\u03ce \u03b7 \u03c1\u03bf\u03ae \u03b5\u03c1\u03b3\u03b1\u03c3\u03af\u03b1\u03c2 \u03b3\u03b9\u03b1 \u03c4\u03b7\u03bd \u03c0\u03c1\u03cc\u03c4\u03b1\u03c3\u03b7 \u03bd\u03ad\u03c9\u03bd \u03bc\u03b5\u03b8\u03cc\u03b4\u03c9\u03bd \u03b5\u03be\u03b1\u03c1\u03c4\u03ac\u03c4\u03b1\u03b9 \u03b1\u03c0\u03cc \u03c4\u03bf \u03c5\u03c0\u03cc\u03b2\u03b1\u03b8\u03c1\u03bf \u03ba\u03b1\u03b9 \u03c4\u03b7\u03bd \u03b5\u03bc\u03c0\u03b5\u03b9\u03c1\u03af\u03b1 \u03c4\u03bf\u03c5 \u03b5\u03c0\u03b9\u03c3\u03c4\u03ae\u03bc\u03bf\u03bd\u03b1.<\/p>\n\n\n\n<p>\u0393\u03b9\u03b1 \u03c4\u03bf\u03c5\u03c2 \u03b2\u03b9\u03bf\u03bb\u03cc\u03b3\u03bf\u03c5\u03c2, \u03bf\u03b9 \u03b5\u03b9\u03ba\u03cc\u03bd\u03b5\u03c2 \u03c4\u03c9\u03bd \u03ba\u03c5\u03c4\u03c4\u03ac\u03c1\u03c9\u03bd \u03bc\u03c0\u03bf\u03c1\u03bf\u03cd\u03bd \u03bd\u03b1 \u03c0\u03bf\u03c5\u03bd \u03c0\u03bf\u03bb\u03bb\u03ac \u03b3\u03b9\u03b1 \u03c4\u03bf 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href=\"https:\/\/link.springer.com\/article\/10.1007\/s13346-019-00657-8\">Mendon\u00e7a's<\/a> \u03a3\u03c4\u03cc\u03c7\u03bf\u03c2 \u03ae\u03c4\u03b1\u03bd \u03c4\u03b1 \u03bd\u03b1\u03bd\u03bf\u03c3\u03c9\u03bc\u03b1\u03c4\u03af\u03b4\u03b9\u03b1 \u03bd\u03b1 \u03c0\u03b5\u03c1\u03ac\u03c3\u03bf\u03c5\u03bd \u03c4\u03bf\u03bd \u03c6\u03c1\u03b1\u03b3\u03bc\u03cc \u03ba\u03b1\u03b9 \u03bd\u03b1 \u03c6\u03c4\u03ac\u03c3\u03bf\u03c5\u03bd \u03c3\u03c4\u03bf\u03bd \u03b5\u03b3\u03ba\u03ad\u03c6\u03b1\u03bb\u03bf, \u03b3\u03b9\u03b1 \u03bd\u03b1 \u03bc\u03b5\u03c4\u03b1\u03c6\u03ad\u03c1\u03bf\u03c5\u03bd \u03c6\u03ac\u03c1\u03bc\u03b1\u03ba\u03b1 \u03ae \u03b3\u03bf\u03bd\u03af\u03b4\u03b9\u03b1 \u03c3\u03b5 \u03bc\u03b9\u03b1 \u03bc\u03b5\u03bb\u03bb\u03bf\u03bd\u03c4\u03b9\u03ba\u03ae \u03b5\u03c6\u03b1\u03c1\u03bc\u03bf\u03b3\u03ae.<\/p>\n\n\n\n<p>\u0398\u03ad\u03bb\u03bf\u03bd\u03c4\u03b1\u03c2 \u03bd\u03b1 \u03b4\u03bf\u03c5\u03bd \u03b1\u03bd \u03c4\u03b1 \u03bd\u03b1\u03bd\u03bf\u03c3\u03c9\u03bc\u03b1\u03c4\u03af\u03b4\u03b9\u03b1 \u03c5\u03c0\u03ae\u03c1\u03c7\u03b1\u03bd \u03c3\u03c4\u03bf \u03b5\u03b3\u03ba\u03b5\u03c6\u03b1\u03bb\u03b9\u03ba\u03cc \u03c0\u03b1\u03c1\u03ad\u03b3\u03c7\u03c5\u03bc\u03b1, \u03bf\u03b9 \u03c3\u03c5\u03b3\u03b3\u03c1\u03b1\u03c6\u03b5\u03af\u03c2 \u03c7\u03c1\u03b7\u03c3\u03b9\u03bc\u03bf\u03c0\u03bf\u03af\u03b7\u03c3\u03b1\u03bd \u03ad\u03bd\u03b1\u03bd \u03b4\u03b5\u03af\u03ba\u03c4\u03b7 \u03b5\u03bd\u03b4\u03bf\u03b8\u03b7\u03bb\u03b9\u03b1\u03ba\u03ce\u03bd \u03ba\u03c5\u03c4\u03c4\u03ac\u03c1\u03c9\u03bd \u03b3\u03b9\u03b1 \u03c4\u03b1 \u03b1\u03b3\u03b3\u03b5\u03af\u03b1 \u03c0\u03bf\u03c5 \u03bf\u03bd\u03bf\u03bc\u03ac\u03b6\u03b5\u03c4\u03b1\u03b9 RECA-1 (\u03b1\u03c0\u03b5\u03b9\u03ba\u03bf\u03bd\u03af\u03b6\u03b5\u03c4\u03b1\u03b9 \u03bc\u03b5 \u03ba\u03cc\u03ba\u03ba\u03b9\u03bd\u03bf \u03c7\u03c1\u03ce\u03bc\u03b1), \u03b5\u03bd\u03ce \u03bf\u03b9 \u03c0\u03c5\u03c1\u03ae\u03bd\u03b5\u03c2 \u03c4\u03c9\u03bd \u03ba\u03c5\u03c4\u03c4\u03ac\u03c1\u03c9\u03bd \u03c7\u03c1\u03c9\u03bc\u03b1\u03c4\u03af\u03c3\u03c4\u03b7\u03ba\u03b1\u03bd \u03bc\u03b5 \u03bc\u03b9\u03b1 \u03c7\u03c1\u03c9\u03c3\u03c4\u03b9\u03ba\u03ae \u03c0\u03bf\u03c5 \u03bf\u03bd\u03bf\u03bc\u03ac\u03b6\u03b5\u03c4\u03b1\u03b9 DAPI (4\u2032,6-\u03b4\u03b9\u03b1\u03bc\u03b9\u03b4\u03b9\u03bd\u03bf-2-\u03c6\u03b1\u03b9\u03bd\u03c5\u03bb\u03b9\u03bd\u03b4\u03cc\u03bb\u03b7), \u03b7 \u03bf\u03c0\u03bf\u03af\u03b1 \u03b5\u03af\u03bd\u03b1\u03b9 \u03bc\u03c0\u03bb\u03b5. \u039c\u03c0\u03bf\u03c1\u03bf\u03cd\u03bc\u03b5 \u03b5\u03c0\u03af\u03c3\u03b7\u03c2 \u03bd\u03b1 \u03c0\u03b1\u03c1\u03b1\u03c4\u03b7\u03c1\u03ae\u03c3\u03bf\u03c5\u03bc\u03b5 \u03bc\u03b9\u03ba\u03c1\u03ad\u03c2 \u03c0\u03c1\u03ac\u03c3\u03b9\u03bd\u03b5\u03c2 \u03ba\u03bf\u03c5\u03ba\u03ba\u03af\u03b4\u03b5\u03c2 \u03b3\u03b9\u03b1 \u03c4\u03b1 \u03bd\u03b1\u03bd\u03bf\u03c3\u03c9\u03bc\u03b1\u03c4\u03af\u03b4\u03b9\u03b1 \u03ad\u03be\u03c9 \u03b1\u03c0\u03cc \u03c4\u03b1 \u03b1\u03b3\u03b3\u03b5\u03af\u03b1, \u03c0\u03c1\u03ac\u03b3\u03bc\u03b1 \u03c0\u03bf\u03c5 \u03c3\u03b7\u03bc\u03b1\u03af\u03bd\u03b5\u03b9 \u03cc\u03c4\u03b9 \u03ad\u03c6\u03c4\u03b1\u03c3\u03b1\u03bd \u03c3\u03c4\u03bf \u03b5\u03b3\u03ba\u03b5\u03c6\u03b1\u03bb\u03b9\u03ba\u03cc \u03c0\u03b1\u03c1\u03ad\u03b3\u03c7\u03c5\u03bc\u03b1.<\/p>\n\n\n\n<p>\u0394\u03b5\u03af\u03c4\u03b5 \u03c4\u03bf \u03c0\u03b1\u03c1\u03b1\u03ba\u03ac\u03c4\u03c9 infographic \u03bc\u03b5 \u03bc\u03b9\u03b1 \u03b5\u03b9\u03ba\u03cc\u03bd\u03b1 \u03b1\u03bd\u03b1\u03c0\u03b1\u03c1\u03ac\u03c3\u03c4\u03b1\u03c3\u03b7\u03c2.<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-large\"><a href=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/06\/preview-347880-7.png\"><img decoding=\"async\" loading=\"lazy\" width=\"788\" height=\"1024\" src=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/06\/preview-347880-7-788x1024.png\" alt=\"Mind the Graph infographic \u03c0\u03ce\u03c2 \u03bd\u03b1 \u03c3\u03c7\u03b5\u03b4\u03b9\u03ac\u03c3\u03b5\u03c4\u03b5 \u03ad\u03bd\u03b1 \u03c0\u03ac\u03bd\u03b5\u03bb \u03b3\u03b9\u03b1 \u03b1\u03bd\u03bf\u03c3\u03bf\u03c6\u03b8\u03bf\u03c1\u03b9\u03c3\u03bc\u03cc\" class=\"wp-image-12980\" srcset=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/06\/preview-347880-7-788x1024.png 788w, https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/06\/preview-347880-7-231x300.png 231w, https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/06\/preview-347880-7-768x998.png 768w, https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/06\/preview-347880-7-1182x1536.png 1182w, https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/06\/preview-347880-7-1575x2048.png 1575w, https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/06\/preview-347880-7-9x12.png 9w, https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2021\/06\/preview-347880-7-77x100.png 77w\" sizes=\"(max-width: 788px) 100vw, 788px\" \/><\/a><\/figure><\/div>\n\n\n<p>\u0391\u03c2 \u03ba\u03b1\u03c4\u03b1\u03bd\u03bf\u03ae\u03c3\u03bf\u03c5\u03bc\u03b5 \u03c4\u03b9 \u03ba\u03ac\u03bd\u03b5\u03b9 \u03c4\u03bf \u03b1\u03bd\u03c4\u03af\u03c3\u03c9\u03bc\u03b1 \u03b3\u03b9\u03b1 \u03c4\u03bf RECA-1 (\u03ba\u03cc\u03ba\u03ba\u03b9\u03bd\u03bf).<\/p>\n\n\n\n<p>\u0391\u03c5\u03c4\u03ac \u03c4\u03b1 \u03b1\u03bd\u03c4\u03b9\u03c3\u03ce\u03bc\u03b1\u03c4\u03b1 \u03ad\u03c7\u03bf\u03c5\u03bd \u03c3\u03c7\u03b5\u03b4\u03b9\u03b1\u03c3\u03c4\u03b5\u03af \u03b3\u03b9\u03b1 \u03bd\u03b1 \u03c7\u03c1\u03b7\u03c3\u03b9\u03bc\u03b5\u03cd\u03bf\u03c5\u03bd \u03c9\u03c2 \u03b5\u03b9\u03b4\u03b9\u03ba\u03bf\u03af \u03b1\u03bd\u03b9\u03c7\u03bd\u03b5\u03c5\u03c4\u03ad\u03c2 \u03ba\u03b1\u03b9 \u03c3\u03c4\u03bf\u03c7\u03b5\u03cd\u03bf\u03c5\u03bd \u03ad\u03bd\u03b1 \u03c3\u03c5\u03b3\u03ba\u03b5\u03ba\u03c1\u03b9\u03bc\u03ad\u03bd\u03bf \u03b1\u03bd\u03c4\u03b9\u03b3\u03cc\u03bd\u03bf (\u03c3\u03c4\u03b7\u03bd \u03c0\u03b5\u03c1\u03af\u03c0\u03c4\u03c9\u03c3\u03ae \u03bc\u03b1\u03c2, \u03c4\u03b7\u03bd \u03c0\u03c1\u03c9\u03c4\u03b5\u0390\u03bd\u03b7 RECA-1).<\/p>\n\n\n\n<p>\u039c\u03c0\u03bf\u03c1\u03bf\u03cd\u03bd \u03bd\u03b1 \u03b5\u03c0\u03b9\u03c3\u03b7\u03bc\u03b1\u03bd\u03b8\u03bf\u03cd\u03bd \u03bc\u03b5 \u03c6\u03b8\u03bf\u03c1\u03b9\u03bf\u03c6\u03cc\u03c1\u03bf \u03ae \u03bd\u03b1 \u03b1\u03bd\u03b1\u03b3\u03bd\u03c9\u03c1\u03b9\u03c3\u03c4\u03bf\u03cd\u03bd \u03b1\u03c1\u03b3\u03cc\u03c4\u03b5\u03c1\u03b1 \u03b1\u03c0\u03cc \u03ad\u03bd\u03b1 \u03b4\u03b5\u03c5\u03c4\u03b5\u03c1\u03bf\u03b3\u03b5\u03bd\u03ad\u03c2 \u03b1\u03bd\u03c4\u03af\u03c3\u03c9\u03bc\u03b1 \u03c3\u03c5\u03bd\u03b4\u03b5\u03b4\u03b5\u03bc\u03ad\u03bd\u03bf \u03bc\u03b5 \u03c6\u03b8\u03bf\u03c1\u03b9\u03bf\u03c6\u03cc\u03c1\u03bf.<\/p>\n\n\n\n<p>\u0395\u03c0\u03bf\u03bc\u03ad\u03bd\u03c9\u03c2, \u03bc\u03b5\u03c4\u03ac \u03c4\u03b7 \u03b4\u03b9\u03ad\u03b3\u03b5\u03c1\u03c3\u03b7 \u03c4\u03bf\u03c5 \u03b4\u03b5\u03af\u03b3\u03bc\u03b1\u03c4\u03bf\u03c2 \u03bc\u03b5 \u03bc\u03b9\u03b1 \u03c0\u03b7\u03b3\u03ae \u03c6\u03c9\u03c4\u03cc\u03c2, <a href=\"https:\/\/onlinelibrary.wiley.com\/doi\/abs\/10.1002\/jobm.3620300304\">\u03b7 \u03c3\u03c5\u03b3\u03ba\u03b5\u03ba\u03c1\u03b9\u03bc\u03ad\u03bd\u03b7 \u03c0\u03c1\u03c9\u03c4\u03b5\u0390\u03bd\u03b7 \u03c0\u03bf\u03c5 \u03b1\u03bd\u03b1\u03b6\u03b7\u03c4\u03ac\u03c4\u03b5 \u03b8\u03b1 \u03b1\u03bd\u03b1\u03b3\u03bd\u03c9\u03c1\u03af\u03b6\u03b5\u03c4\u03b1\u03b9 \u03c3\u03c4\u03bf \u03b4\u03b5\u03af\u03b3\u03bc\u03b1 \u03c3\u03b1\u03c2 \u03b1\u03c0\u03cc \u03c4\u03b7\u03bd \u03b5\u03ba\u03c0\u03bf\u03bc\u03c0\u03ae \u03c6\u03c9\u03c4\u03cc\u03c2 \u03c3\u03b5 \u03ad\u03bd\u03b1 \u03c3\u03c5\u03b3\u03ba\u03b5\u03ba\u03c1\u03b9\u03bc\u03ad\u03bd\u03bf \u03bc\u03ae\u03ba\u03bf\u03c2 \u03ba\u03cd\u03bc\u03b1\u03c4\u03bf\u03c2<\/a>.<\/p>\n\n\n\n<p>\u03a3\u03c4\u03b7\u03bd \u03c0\u03b5\u03c1\u03af\u03c0\u03c4\u03c9\u03c3\u03b7 \u03c4\u03b7\u03c2 DAPI, \u03b7 \u03c7\u03c1\u03c9\u03c3\u03c4\u03b9\u03ba\u03ae \u03b1\u03c5\u03c4\u03ae \u03b5\u03af\u03bd\u03b1\u03b9 \u03b1\u03bd\u03c4\u03b9\u03c7\u03c1\u03c9\u03c3\u03c4\u03b9\u03ba\u03ae \u03c4\u03c9\u03bd \u03c0\u03c5\u03c1\u03ae\u03bd\u03c9\u03bd \u03ba\u03b1\u03b9 \u03c4\u03c9\u03bd \u03bd\u03bf\u03c5\u03ba\u03bb\u03b5\u03bf\u03c3\u03c9\u03bc\u03ac\u03c4\u03c9\u03bd \u03ba\u03b1\u03b9 \u03b5\u03ba\u03c0\u03ad\u03bc\u03c0\u03b5\u03b9 \u03bc\u03c0\u03bb\u03b5 \u03c6\u03b8\u03bf\u03c1\u03b9\u03c3\u03bc\u03cc \u03cc\u03c4\u03b1\u03bd \u03c3\u03c5\u03bd\u03b4\u03ad\u03b5\u03c4\u03b1\u03b9 \u03bc\u03b5 \u03c0\u03b5\u03c1\u03b9\u03bf\u03c7\u03ad\u03c2 AT \u03c4\u03bf\u03c5 DNA.<\/p>\n\n\n\n<p class=\"has-large-font-size\"><strong>\u03a0\u03ce\u03c2 \u03bd\u03b1 \u03c3\u03c7\u03b5\u03b4\u03b9\u03ac\u03c3\u03b5\u03c4\u03b5 \u03ad\u03bd\u03b1 \u03c0\u03ac\u03bd\u03b5\u03bb \u03b3\u03b9\u03b1 \u03b1\u03bd\u03bf\u03c3\u03bf\u03c6\u03b8\u03bf\u03c1\u03b9\u03c3\u03bc\u03cc; <\/strong><\/p>\n\n\n\n<p class=\"has-large-font-size\"><strong>\u039e\u03b5\u03ba\u03b9\u03bd\u03ae\u03c3\u03c4\u03b5 \u03bc\u03b5 \u03b1\u03c5\u03c4\u03ac \u03c4\u03b1 \u03b2\u03ae\u03bc\u03b1\u03c4\u03b1:<\/strong><\/p>\n\n\n\n<ol>\n<li>\u0391\u03b3\u03bf\u03c1\u03ac\u03c3\u03c4\u03b5 (\u03ae \u03b4\u03b1\u03bd\u03b5\u03b9\u03c3\u03c4\u03b5\u03af\u03c4\u03b5! \u0397 \u03b5\u03c0\u03b9\u03c3\u03c4\u03ae\u03bc\u03b7 \u03c0\u03c1\u03ad\u03c0\u03b5\u03b9 \u03bd\u03b1 \u03b5\u03af\u03bd\u03b1\u03b9 \u03c0\u03bf\u03bb\u03cd \u03c3\u03c5\u03bd\u03b5\u03c1\u03b3\u03b1\u03c4\u03b9\u03ba\u03ae!) \u03b1\u03bd\u03c4\u03b9\u03c3\u03ce\u03bc\u03b1\u03c4\u03b1 \u03ba\u03b1\u03b9 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href=\"https:\/\/www.thermofisher.com\/order\/spectra-viewer\">\u03a0\u03c1\u03cc\u03b3\u03c1\u03b1\u03bc\u03bc\u03b1 \u03c0\u03c1\u03bf\u03b2\u03bf\u03bb\u03ae\u03c2 \u03c6\u03b1\u03c3\u03bc\u03ac\u03c4\u03c9\u03bd \u03c6\u03b8\u03bf\u03c1\u03b9\u03c3\u03bc\u03bf\u03cd<\/a> \u03b5\u03af\u03bd\u03b1\u03b9 \u03bc\u03b9\u03b1 \u03b5\u03be\u03b1\u03b9\u03c1\u03b5\u03c4\u03b9\u03ba\u03ae \u03b5\u03c0\u03b9\u03bb\u03bf\u03b3\u03ae. \u039a\u03b1\u03bb\u03cd\u03c0\u03c4\u03bf\u03c5\u03bd \u03c3\u03c7\u03b5\u03b4\u03cc\u03bd \u03cc\u03bb\u03b1 \u03c4\u03b1 \u03b4\u03b9\u03b1\u03b8\u03ad\u03c3\u03b9\u03bc\u03b1 \u03c6\u03b8\u03bf\u03c1\u03b9\u03bf\u03c6\u03cc\u03c1\u03b1!<\/li>\n<\/ol>\n\n\n\n<p>\u03a4\u03ad\u03bb\u03bf\u03c2, \u03b5\u03bb\u03ad\u03b3\u03be\u03c4\u03b5 \u03ad\u03bd\u03b1 \u03c0\u03b1\u03c1\u03ac\u03b4\u03b5\u03b9\u03b3\u03bc\u03b1 \u03b3\u03b9\u03b1 \u03ad\u03bd\u03b1 \u03c5\u03c0\u03bf\u03b8\u03b5\u03c4\u03b9\u03ba\u03cc \u03c0\u03b5\u03af\u03c1\u03b1\u03bc\u03b1 \u03cc\u03c0\u03bf\u03c5 \u03ad\u03c7\u03bf\u03c5\u03bc\u03b5 Hoechst 33258 \u03b3\u03b9\u03b1 \u03c4\u03b1 \u03bd\u03bf\u03c5\u03ba\u03bb\u03b5\u03ca\u03ba\u03ac \u03bf\u03be\u03ad\u03b1 \u03ba\u03b1\u03b9 \u03ad\u03bd\u03b1 \u03c0\u03c1\u03c9\u03c4\u03bf\u03b3\u03b5\u03bd\u03ad\u03c2 \u03b1\u03bd\u03c4\u03af\u03c3\u03c9\u03bc\u03b1 \u03ad\u03bd\u03b1\u03bd\u03c4\u03b9 \u03c4\u03bf\u03c5 RECA-1 \u03b5\u03c0\u03b9\u03c3\u03b7\u03bc\u03b1\u03c3\u03bc\u03ad\u03bd\u03bf \u03bc\u03b5 \u03ad\u03bd\u03b1 \u03b4\u03b5\u03c5\u03c4\u03b5\u03c1\u03bf\u03b3\u03b5\u03bd\u03ad\u03c2 \u03b1\u03bd\u03c4\u03af\u03c3\u03c9\u03bc\u03b1 Alexa Fluor 647.<\/p>\n\n\n\n<p>\u0399\u03b4\u03b1\u03bd\u03b9\u03ba\u03ac, \u03b8\u03b1 \u03c7\u03c1\u03b7\u03c3\u03b9\u03bc\u03bf\u03c0\u03bf\u03b9\u03bf\u03cd\u03c3\u03b1\u03bc\u03b5 \u03ad\u03bd\u03b1 \u03bc\u03b9\u03ba\u03c1\u03bf\u03c3\u03ba\u03cc\u03c0\u03b9\u03bf \u03b4\u03b9\u03b1\u03b8\u03ad\u03c3\u03b9\u03bc\u03bf \u03bc\u03b5 \u03ba\u03cd\u03b2\u03bf DAPI (\u03b4\u03b9\u03ad\u03b3\u03b5\u03c1\u03c3\u03b7 377\/50 \u03ba\u03b1\u03b9 \u03b5\u03ba\u03c0\u03bf\u03bc\u03c0\u03ae 447\/60) \u03ba\u03b1\u03b9 \u03ba\u03cd\u03b2\u03bf CY5 (\u03b4\u03b9\u03ad\u03b3\u03b5\u03c1\u03c3\u03b7 628\/40 \u03ba\u03b1\u03b9 \u03b5\u03ba\u03c0\u03bf\u03bc\u03c0\u03ae 685\/40). \u038c\u03bb\u03b5\u03c2 \u03b1\u03c5\u03c4\u03ad\u03c2 \u03bf\u03b9 \u03c0\u03bb\u03b7\u03c1\u03bf\u03c6\u03bf\u03c1\u03af\u03b5\u03c2 \u03b5\u03b9\u03c3\u03ac\u03b3\u03bf\u03bd\u03c4\u03b1\u03b9 \u03c3\u03c4\u03bf <a href=\"https:\/\/www.thermofisher.com\/order\/spectra-viewer\">\u03a0\u03c1\u03cc\u03b3\u03c1\u03b1\u03bc\u03bc\u03b1 \u03c0\u03c1\u03bf\u03b2\u03bf\u03bb\u03ae\u03c2 \u03c6\u03b1\u03c3\u03bc\u03ac\u03c4\u03c9\u03bd \u03c6\u03b8\u03bf\u03c1\u03b9\u03c3\u03bc\u03bf\u03cd<\/a> \u03ba\u03b1\u03b9 \u03c0\u03ae\u03c1\u03b1\u03bc\u03b5 \u03c4\u03b1 \u03c6\u03ac\u03c3\u03bc\u03b1\u03c4\u03b1 \u03ba\u03b1\u03b9 \u03b3\u03b9\u03b1 \u03c4\u03b9\u03c2 \u03b4\u03cd\u03bf \u03c7\u03c1\u03c9\u03c3\u03c4\u03b9\u03ba\u03ad\u03c2, \u03ba\u03b1\u03b8\u03ce\u03c2 \u03ba\u03b1\u03b9 \u03c4\u03b1 \u03b5\u03cd\u03c1\u03b7 \u03b6\u03ce\u03bd\u03b7\u03c2 \u03ba\u03b1\u03b9 \u03b3\u03b9\u03b1 \u03c4\u03bf\u03c5\u03c2 \u03b4\u03cd\u03bf \u03ba\u03cd\u03b2\u03bf\u03c5\u03c2 (\u03b4\u03b5\u03af\u03c4\u03b5 \u03c4\u03bf \u03c6\u03ac\u03c3\u03bc\u03b1 \u03c3\u03c4\u03bf \u03c0\u03b1\u03c1\u03b1\u03c0\u03ac\u03bd\u03c9 infographic).<\/p>\n\n\n\n<p>\u0391\u03c5\u03c4\u03cc \u03c4\u03bf \u03c5\u03c0\u03bf\u03b8\u03b5\u03c4\u03b9\u03ba\u03cc \u03b4\u03bf\u03ba\u03af\u03bc\u03b9\u03bf \u03b5\u03af\u03bd\u03b1\u03b9 \u03ad\u03bd\u03b1 \u03ba\u03b1\u03bb\u03cc \u03c0\u03b1\u03c1\u03ac\u03b4\u03b5\u03b9\u03b3\u03bc\u03b1 \u03cc\u03c0\u03bf\u03c5 \u03c4\u03b1 \u03c6\u03ac\u03c3\u03bc\u03b1\u03c4\u03b1 \u03c4\u03c9\u03bd \u03c6\u03b8\u03bf\u03c1\u03b9\u03bf\u03c6\u03cc\u03c1\u03c9\u03bd \u03b1\u03bd\u03ae\u03ba\u03bf\u03c5\u03bd \u03bc\u03ad\u03c3\u03b1 \u03c3\u03c4\u03b1 \u03c6\u03af\u03bb\u03c4\u03c1\u03b1 \u03b4\u03b9\u03ad\u03b3\u03b5\u03c1\u03c3\u03b7\u03c2 \u03ba\u03b1\u03b9 \u03b5\u03ba\u03c0\u03bf\u03bc\u03c0\u03ae\u03c2, \u03ba\u03b1\u03b8\u03b9\u03c3\u03c4\u03ce\u03bd\u03c4\u03b1\u03c2 \u03b4\u03c5\u03bd\u03b1\u03c4\u03ae \u03b3\u03b9\u03b1 \u03c4\u03bf\u03bd \u03b5\u03c1\u03b5\u03c5\u03bd\u03b7\u03c4\u03ae \u03c4\u03b7\u03bd \u03ba\u03b1\u03c4\u03b1\u03b3\u03c1\u03b1\u03c6\u03ae \u03c4\u03c9\u03bd \u03b4\u03b5\u03b9\u03b3\u03bc\u03ac\u03c4\u03c9\u03bd \u03c4\u03bf\u03c5 \u03bc\u03b5 \u03c4\u03bf\u03bd \u03ba\u03b1\u03bb\u03cd\u03c4\u03b5\u03c1\u03bf \u03b4\u03c5\u03bd\u03b1\u03c4\u03cc \u03c4\u03c1\u03cc\u03c0\u03bf.<\/p>\n\n\n\n<p>\u03a4\u03ce\u03c1\u03b1, \u03ae\u03c1\u03b8\u03b5 \u03b7 \u03ce\u03c1\u03b1 \u03bd\u03b1 \u03c0\u03ac\u03c4\u03b5 \u03c3\u03c4\u03bf \u03b5\u03c1\u03b3\u03b1\u03c3\u03c4\u03ae\u03c1\u03b9\u03bf \u03ba\u03b1\u03b9 \u03bd\u03b1 \u03c4\u03b1 \u03b5\u03c6\u03b1\u03c1\u03bc\u03cc\u03c3\u03b5\u03c4\u03b5 \u03cc\u03bb\u03b1 \u03c3\u03c4\u03b7\u03bd \u03c0\u03c1\u03ac\u03be\u03b7!<\/p>\n\n\n\n<p>\u0395\u03bb\u03c0\u03af\u03b6\u03c9 \u03b1\u03c5\u03c4\u03ad\u03c2 \u03bf\u03b9 \u03c3\u03c5\u03bc\u03b2\u03bf\u03c5\u03bb\u03ad\u03c2 \u03bd\u03b1 \u03c3\u03b1\u03c2 \u03b2\u03bf\u03b7\u03b8\u03ae\u03c3\u03bf\u03c5\u03bd \u03c3\u03c4\u03bf \u03b5\u03c0\u03cc\u03bc\u03b5\u03bd\u03bf \u03b5\u03c1\u03b3\u03b1\u03c3\u03c4\u03b7\u03c1\u03b9\u03b1\u03ba\u03cc \u03c3\u03b1\u03c2 \u03c0\u03b5\u03af\u03c1\u03b1\u03bc\u03b1. \u039a\u03b1\u03bb\u03ae \u03c4\u03cd\u03c7\u03b7!<\/p>\n\n\n\n<p><strong>\u0391\u03bd\u03b1\u03c6\u03bf\u03c1\u03ad\u03c2:<\/strong><\/p>\n\n\n\n<ol>\n<li><a href=\"https:\/\/www.zotero.org\/google-docs\/?imatmT\">\u0395\u03b9\u03c3\u03b1\u03b3\u03c9\u03b3\u03ae \u03c3\u03c4\u03b7 \u03bc\u03b9\u03ba\u03c1\u03bf\u03c3\u03ba\u03bf\u03c0\u03af\u03b1 \u03c6\u03b8\u03bf\u03c1\u03b9\u03c3\u03bc\u03bf\u03cd. <em>\u039c\u03b9\u03ba\u03c1\u03bf\u03c3\u03ba\u03bf\u03c0\u03af\u03b1 \u03c4\u03b7\u03c2 NikonU <\/em><\/a><a href=\"https:\/\/www.microscopyu.com\/techniques\/fluorescence\/introduction-to-fluorescence-microscopy\">https:\/\/www.microscopyu.com\/techniques\/fluorescence\/introduction-to-fluorescence-microscopy<\/a><a href=\"https:\/\/www.zotero.org\/google-docs\/?imatmT\">. \u03a0\u03c1\u03cc\u03c3\u03b2\u03b1\u03c3\u03b7 2021-04-11 17:20:40.<\/a><\/li>\n<\/ol>","protected":false},"excerpt":{"rendered":"<p>\u0388\u03bd\u03b1 \u03bc\u03b5\u03b3\u03ac\u03bb\u03bf \u03bc\u03ad\u03c1\u03bf\u03c2 \u03c4\u03b7\u03c2 \u03ba\u03b1\u03b8\u03b7\u03bc\u03b5\u03c1\u03b9\u03bd\u03cc\u03c4\u03b7\u03c4\u03b1\u03c2 \u03c4\u03bf\u03c5 \u03b5\u03c0\u03b9\u03c3\u03c4\u03ae\u03bc\u03bf\u03bd\u03b1 \u03b5\u03af\u03bd\u03b1\u03b9 \u03bd\u03b1 \u03c3\u03c7\u03b5\u03b4\u03b9\u03ac\u03b6\u03b5\u03b9 \u03ba\u03b1\u03b9 \u03bd\u03b1 \u03b5\u03ba\u03c4\u03b5\u03bb\u03b5\u03af \u03c0\u03b5\u03b9\u03c1\u03ac\u03bc\u03b1\u03c4\u03b1. \u039f \u03c3\u03c5\u03bd\u03b4\u03c5\u03b1\u03c3\u03bc\u03cc\u03c2 \u03b5\u03c1\u03b3\u03b1\u03c3\u03c4\u03b7\u03c1\u03b9\u03b1\u03ba\u03ce\u03bd \u03c4\u03b5\u03c7\u03bd\u03b9\u03ba\u03ce\u03bd \u03b8\u03b1 \u03b1\u03c0\u03b1\u03bd\u03c4\u03ae\u03c3\u03b5\u03b9 \u03c3\u03c4\u03b1 \u03c0\u03b5\u03c1\u03b9\u03c3\u03c3\u03cc\u03c4\u03b5\u03c1\u03b1 \u03b1\u03c0\u03cc \u03c4\u03b1 \u03b5\u03c1\u03c9\u03c4\u03ae\u03bc\u03b1\u03c4\u03b1 \u03c0\u03bf\u03c5 \u03c0\u03c1\u03bf\u03c4\u03b5\u03af\u03bd\u03bf\u03c5\u03bd \u03bf\u03b9 \u03b5\u03c0\u03b9\u03c3\u03c4\u03ae\u03bc\u03bf\u03bd\u03b5\u03c2, \u03b5\u03bd\u03ce \u03b7 \u03c1\u03bf\u03ae \u03b5\u03c1\u03b3\u03b1\u03c3\u03af\u03b1\u03c2 \u03b3\u03b9\u03b1 \u03c4\u03b7\u03bd \u03c0\u03c1\u03cc\u03c4\u03b1\u03c3\u03b7 \u03bd\u03ad\u03c9\u03bd \u03bc\u03b5\u03b8\u03cc\u03b4\u03c9\u03bd \u03b5\u03be\u03b1\u03c1\u03c4\u03ac\u03c4\u03b1\u03b9 \u03b1\u03c0\u03cc \u03c4\u03bf \u03c5\u03c0\u03cc\u03b2\u03b1\u03b8\u03c1\u03bf \u03ba\u03b1\u03b9 \u03c4\u03b7\u03bd \u03b5\u03bc\u03c0\u03b5\u03b9\u03c1\u03af\u03b1 \u03c4\u03bf\u03c5 \u03b5\u03c0\u03b9\u03c3\u03c4\u03ae\u03bc\u03bf\u03bd\u03b1. \u0393\u03b9\u03b1 \u03c4\u03bf\u03c5\u03c2 \u03b2\u03b9\u03bf\u03bb\u03cc\u03b3\u03bf\u03c5\u03c2, \u03bf\u03b9 \u03b5\u03b9\u03ba\u03cc\u03bd\u03b5\u03c2 \u03c4\u03c9\u03bd \u03ba\u03c5\u03c4\u03c4\u03ac\u03c1\u03c9\u03bd \u03bc\u03c0\u03bf\u03c1\u03bf\u03cd\u03bd \u03bd\u03b1 \u03c0\u03bf\u03c5\u03bd \u03c0\u03bf\u03bb\u03bb\u03ac \u03b3\u03b9\u03b1 \u03c4\u03bf \u03c4\u03b9 \u03c3\u03c5\u03bc\u03b2\u03b1\u03af\u03bd\u03b5\u03b9 \u03bc\u03b5 \u03c4\u03bf [...]<\/p>","protected":false},"author":18,"featured_media":12982,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[66,959,958,28,38],"tags":[51,554,962],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v19.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Microscopy: How Scientists Use Fluorescence To See Inside Cells -<\/title>\n<meta name=\"description\" content=\"Fluorescence microscopy can say a lot about a cell process through image. That is why knowing how to design the experiment is so important.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mindthegraph.com\/blog\/el\/microscopy-how-scientists-use-fluorescence-to-see-inside-cells\/\" \/>\n<meta property=\"og:locale\" content=\"el_GR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Microscopy: How Scientists Use Fluorescence To See Inside Cells\" \/>\n<meta property=\"og:description\" content=\"Fluorescence microscopy can say a lot about a cell process through image. 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