{"id":16636,"date":"2022-09-10T18:27:00","date_gmt":"2022-09-10T21:27:00","guid":{"rendered":"https:\/\/mindthegraph.com\/blog\/?p=16636"},"modified":"2023-03-17T14:47:45","modified_gmt":"2023-03-17T17:47:45","slug":"success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids","status":"publish","type":"post","link":"https:\/\/mindthegraph.com\/blog\/da\/succes-case-fgf-signalering-i-selvfornyelsen-af-koloncancer-organoider\/","title":{"rendered":"FGF-signalering i selvfornyelsen af organoider til tyktarmskr\u00e6ft Paper Review"},"content":{"rendered":"

Den deaktiverede sf\u00e6roid- og organoiddannelse ved FGFR-h\u00e6mning i patientafledte CRC-celler er meget lettere at forst\u00e5 ved hj\u00e6lp af Mind the Graph's illustrationer. <\/p>\n\n\n\n

Det skema, som forfatteren har lavet, viser, at de kirurgiske biopsier blev enzymatisk ford\u00f8jet for at opn\u00e5 en suspension af enkeltceller. De naive usorterede celler blev dyrket i FGF2-holdigt CSC-medium eller med FGFR-inhibitoren SU-5402.<\/p>\n\n\n\n

Fibroblastv\u00e6kstfaktor 2's (FGF2) rolle i selvfornyelsen af stamceller fra kolorektal cancer er blevet unders\u00f8gt.<\/strong><\/h2>\n\n\n\n

I \"FGF Signalling in the Self-Renewal of Colon Cancer Organoids\" rapporterede J\u00f6rg Otte og kolleger (2019), at n\u00e5r naive enkeltceller blev indlejret i Matrigel og dyrket i CSC-mediet, blev der observeret en mere kompleks selvorganisering hos alle de analyserede patienter. <\/p>\n\n\n\n

Gener, der er direkte forbundet med EMT sammen med en mesenchymal og selvfornyende f\u00e6notype (TGFB3, ID1, ID2 og ID4), viste sig at v\u00e6re overudtrykt i organoider. <\/p>\n\n\n\n

Mens FGFR-h\u00e6mning f\u00f8rte til en \u00f8get MAPK-signalering, observerede de induceret differentiering, et tab af de fleste stamcellemark\u00f8rer og en epitelial f\u00e6notype. <\/p>\n\n\n\n

Kr\u00e6ftstamceller er en subpopulation af ondartede celler, der er i stand til at forny sig selv og fungere som en l\u00f8bende kilde til differentierede tumorceller. I nogle protokoller for CSC-kultur er fibroblastv\u00e6kstfaktor 2 en vigtig ingrediens for at opretholde stamceller. <\/p>\n\n\n\n

I CSC-mediumkulturerede organoider p\u00e5viste de derimod induktion af TGF-\u03b2 og af alle fire medlemmer af inhibitor-of-differentiation-genfamilien.<\/p>\n\n\n\n

9 p\u00e5 hinanden f\u00f8lgende, uselekterede CRC-patienter deltog i unders\u00f8gelsen.<\/strong><\/p>\n\n\n\n

Kontroller Originalartikel her<\/a>.<\/p>\n\n\n\n

Grafisk abstrakt drevet af Mind the Graph<\/strong><\/h2>\n\n\n\n
<\/div>\n\n\n
\n
\"\"<\/a>
Fremstillet med Mind the Graph<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

Nogle af deres konklusioner styrker potentielt det, man tidligere har vidst om dette felt: \"De pluripotency-associerede gener NANOG, LIN28 og SALL1 blev udelukkende udtrykt af hESC'er. GO-termerne \"negativ regulering af BMP-signalvejen\" og \"SMAD-proteinsignaltransduktion\" blev p\u00e5vist i vores hESC-kultur,\" foreslog Otte.<\/p>\n\n\n\n

Forfatterne indr\u00f8mmer, at \"GO-term-analyse af dette gen-s\u00e6t gav kun f\u00e5 signifikante resultater p\u00e5 grund af det lave antal almindeligt regulerede gener. De fleste af disse GO-termer for CSC-medium-tilstanden var forbundet med aktiv transkription, celleproliferation eller \u00f8get glukoseoptagelse. <\/p>\n\n\n\n

Efter FGFR-inhibering fandt vi cellecyklusregulatorer samt mange gener, der er annoteret med \"epitelcelledifferentiering\", \"ford\u00f8jelse\" eller \"galdesyre- og galdesalttransport\".\"<\/p>\n\n\n\n

Artiklernes effekt og ydeevne<\/strong><\/h2>\n\n\n\n

Vi kan analysere med Altmetric<\/a> data, at denne artikels online opm\u00e6rksomhedsscore er h\u00f8jere end 68%<\/strong><\/strong> af alle andre artikler af samme alder i Scientific Reports.<\/strong><\/p>\n\n\n\n

<\/div>\n\n\n
\n
\"\"<\/a>
Kilde Altmetri<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

G\u00f8r ogs\u00e5 din artikel mere tilg\u00e6ngelig ved hj\u00e6lp af infografik.<\/p>\n\n\n\n

\n
Pr\u00f8v Mind the Graph<\/strong><\/a><\/div>\n<\/div>\n\n\n\n
<\/div>","protected":false},"excerpt":{"rendered":"

Den deaktiverede sf\u00e6roid- og organoiddannelse ved FGFR-inhibering i patientafledte CRC-celler er lettere at forst\u00e5 med vores illustrationer.<\/p>","protected":false},"author":29,"featured_media":20014,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[195,958,957,1],"tags":[96,554,775,963],"yoast_head":"\nFGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog<\/title>\n<meta name=\"description\" content=\"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mindthegraph.com\/blog\/da\/succes-case-fgf-signalering-i-selvfornyelsen-af-koloncancer-organoider\/\" \/>\n<meta property=\"og:locale\" content=\"da_DK\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog\" \/>\n<meta property=\"og:description\" content=\"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/mindthegraph.com\/blog\/da\/succes-case-fgf-signalering-i-selvfornyelsen-af-koloncancer-organoider\/\" \/>\n<meta property=\"og:site_name\" content=\"Mind the Graph Blog\" \/>\n<meta property=\"article:published_time\" content=\"2022-09-10T21:27:00+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2023-03-17T17:47:45+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2022\/09\/blog_4.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1125\" \/>\n\t<meta property=\"og:image:height\" content=\"614\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Artur Zignani\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:image\" content=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2022\/09\/blog_4.jpg\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Artur Zignani\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"3 minutes\" \/>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog","description":"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/mindthegraph.com\/blog\/da\/succes-case-fgf-signalering-i-selvfornyelsen-af-koloncancer-organoider\/","og_locale":"da_DK","og_type":"article","og_title":"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog","og_description":"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.","og_url":"https:\/\/mindthegraph.com\/blog\/da\/succes-case-fgf-signalering-i-selvfornyelsen-af-koloncancer-organoider\/","og_site_name":"Mind the Graph Blog","article_published_time":"2022-09-10T21:27:00+00:00","article_modified_time":"2023-03-17T17:47:45+00:00","og_image":[{"width":1125,"height":614,"url":"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2022\/09\/blog_4.jpg","type":"image\/jpeg"}],"author":"Artur Zignani","twitter_card":"summary_large_image","twitter_image":"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2022\/09\/blog_4.jpg","twitter_misc":{"Written by":"Artur Zignani","Est. reading time":"3 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/mindthegraph.com\/blog\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/","url":"https:\/\/mindthegraph.com\/blog\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/","name":"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog","isPartOf":{"@id":"https:\/\/mindthegraph.com\/blog\/#website"},"datePublished":"2022-09-10T21:27:00+00:00","dateModified":"2023-03-17T17:47:45+00:00","author":{"@id":"https:\/\/mindthegraph.com\/blog\/#\/schema\/person\/b44995c1d77b52a63b6d11262b740b02"},"description":"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.","breadcrumb":{"@id":"https:\/\/mindthegraph.com\/blog\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/#breadcrumb"},"inLanguage":"da-DK","potentialAction":[{"@type":"ReadAction","target":["https:\/\/mindthegraph.com\/blog\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/mindthegraph.com\/blog\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/mindthegraph.com\/blog\/"},{"@type":"ListItem","position":2,"name":"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review"}]},{"@type":"WebSite","@id":"https:\/\/mindthegraph.com\/blog\/#website","url":"https:\/\/mindthegraph.com\/blog\/","name":"Mind the Graph Blog","description":"Your science can be beautiful!","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/mindthegraph.com\/blog\/?s={search_term_string}"},"query-input":"required name=search_term_string"}],"inLanguage":"da-DK"},{"@type":"Person","@id":"https:\/\/mindthegraph.com\/blog\/#\/schema\/person\/b44995c1d77b52a63b6d11262b740b02","name":"Artur Zignani","image":{"@type":"ImageObject","inLanguage":"da-DK","@id":"https:\/\/mindthegraph.com\/blog\/#\/schema\/person\/image\/","url":"https:\/\/secure.gravatar.com\/avatar\/a211ec97011fe24f7a8e63a7ca799342?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/a211ec97011fe24f7a8e63a7ca799342?s=96&d=mm&r=g","caption":"Artur Zignani"},"url":"https:\/\/mindthegraph.com\/blog\/da\/author\/artur\/"}]}},"_links":{"self":[{"href":"https:\/\/mindthegraph.com\/blog\/da\/wp-json\/wp\/v2\/posts\/16636"}],"collection":[{"href":"https:\/\/mindthegraph.com\/blog\/da\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/mindthegraph.com\/blog\/da\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/mindthegraph.com\/blog\/da\/wp-json\/wp\/v2\/users\/29"}],"replies":[{"embeddable":true,"href":"https:\/\/mindthegraph.com\/blog\/da\/wp-json\/wp\/v2\/comments?post=16636"}],"version-history":[{"count":5,"href":"https:\/\/mindthegraph.com\/blog\/da\/wp-json\/wp\/v2\/posts\/16636\/revisions"}],"predecessor-version":[{"id":27203,"href":"https:\/\/mindthegraph.com\/blog\/da\/wp-json\/wp\/v2\/posts\/16636\/revisions\/27203"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/mindthegraph.com\/blog\/da\/wp-json\/wp\/v2\/media\/20014"}],"wp:attachment":[{"href":"https:\/\/mindthegraph.com\/blog\/da\/wp-json\/wp\/v2\/media?parent=16636"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/mindthegraph.com\/blog\/da\/wp-json\/wp\/v2\/categories?post=16636"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/mindthegraph.com\/blog\/da\/wp-json\/wp\/v2\/tags?post=16636"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}