{"id":16636,"date":"2022-09-10T18:27:00","date_gmt":"2022-09-10T21:27:00","guid":{"rendered":"https:\/\/mindthegraph.com\/blog\/?p=16636"},"modified":"2023-03-17T14:47:45","modified_gmt":"2023-03-17T17:47:45","slug":"success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids","status":"publish","type":"post","link":"https:\/\/mindthegraph.com\/blog\/cs\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/","title":{"rendered":"Signalizace FGF v sebeobnov\u011b organoid\u016f karcinomu tlust\u00e9ho st\u0159eva P\u0159ehled \u010dl\u00e1nk\u016f"},"content":{"rendered":"

Tvorba posti\u017een\u00fdch sf\u00e9roid\u016f a organoid\u016f inhibic\u00ed FGFR v bu\u0148k\u00e1ch CRC odvozen\u00fdch od pacient\u016f je mnohem srozumiteln\u011bj\u0161\u00ed pomoc\u00ed ilustrac\u00ed Mind the Graph. <\/p>\n\n\n\n

Sch\u00e9ma vytvo\u0159en\u00e9 autorem ukazuje, \u017ee chirurgick\u00e9 biopsie byly enzymaticky \u0161t\u011bpeny za \u00fa\u010delem z\u00edsk\u00e1n\u00ed suspenze jednotliv\u00fdch bun\u011bk. Naivn\u00ed net\u0159\u00edd\u011bn\u00e9 bu\u0148ky byly kultivov\u00e1ny v CSC-mediu obsahuj\u00edc\u00edm FGF2 nebo s inhibitorem FGFR SU-5402.<\/p>\n\n\n\n

Byla zkoum\u00e1na role fibroblastov\u00e9ho r\u016fstov\u00e9ho faktoru 2 (FGF2) v sebeobnov\u011b kmenov\u00fdch bun\u011bk kolorekt\u00e1ln\u00edho karcinomu.<\/strong><\/h2>\n\n\n\n

V \u010dl\u00e1nku \"FGF Signalling in the Self-Renewal of Colon Cancer Organoids\" J\u00f6rg Otte a jeho kolegov\u00e9 (2019) uv\u00e1d\u011bj\u00ed, \u017ee kdy\u017e byly naivn\u00ed jednotliv\u00e9 bu\u0148ky vlo\u017eeny do Matrigelu a kultivov\u00e1ny v CSC-mediu, byla u v\u0161ech analyzovan\u00fdch pacient\u016f pozorov\u00e1na komplexn\u011bj\u0161\u00ed sebeorganizace. <\/p>\n\n\n\n

Bylo zji\u0161t\u011bno, \u017ee geny p\u0159\u00edmo spojen\u00e9 s EMT spolu s mezenchym\u00e1ln\u00edm a sebeobnovuj\u00edc\u00edm fenotypem (TGFB3, ID1, ID2 a ID4) jsou v organoidech nadm\u011brn\u011b exprimov\u00e1ny. <\/p>\n\n\n\n

Zat\u00edmco inhibice FGFR vedla k pos\u00edlen\u00ed signalizace MAPK, pozorovali indukovanou diferenciaci, ztr\u00e1tu v\u011bt\u0161iny marker\u016f kmenov\u00fdch bun\u011bk a epiteli\u00e1ln\u00ed fenotyp. <\/p>\n\n\n\n

N\u00e1dorov\u00e9 kmenov\u00e9 bu\u0148ky jsou subpopulac\u00ed malign\u00edch bun\u011bk schopn\u00fdch sebeobnovy a slou\u017e\u00edc\u00edch jako trval\u00fd zdroj diferencovan\u00fdch n\u00e1dorov\u00fdch bun\u011bk. V n\u011bkter\u00fdch protokolech kultivace CSC je Fibroblastov\u00fd r\u016fstov\u00fd faktor 2 d\u016fle\u017eitou slo\u017ekou pro udr\u017een\u00ed kmenovosti. <\/p>\n\n\n\n

V organoidech kultivovan\u00fdch na CSC m\u00e9diu naopak zjistili indukci TGF-\u03b2 a v\u0161ech \u010dty\u0159 \u010dlen\u016f rodiny gen\u016f inhibitor\u016f diferenciace.<\/p>\n\n\n\n

Do v\u00fdzkumu bylo zapojeno 9 po sob\u011b jdouc\u00edch neselektovan\u00fdch pacient\u016f s CRC.<\/strong><\/p>\n\n\n\n

Zkontrolujte p\u016fvodn\u00ed \u010dl\u00e1nek zde<\/a>.<\/p>\n\n\n\n

Grafick\u00e9 abstraktum poh\u00e1n\u011bn\u00e9 Mind the Graph<\/strong><\/h2>\n\n\n\n
<\/div>\n\n\n
\n
\"\"<\/a>
Vyrobeno s Mind the Graph<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

N\u011bkter\u00e9 z jejich z\u00e1v\u011br\u016f potenci\u00e1ln\u011b posiluj\u00ed dosavadn\u00ed poznatky o t\u00e9to oblasti: \"Geny souvisej\u00edc\u00ed s pluripotenc\u00ed NANOG, LIN28 a SALL1 byly exprimov\u00e1ny v\u00fdhradn\u011b hESC. V na\u0161\u00ed kultu\u0159e hESC byly zji\u0161t\u011bny GO-term\u00edny \"negativn\u00ed regulace sign\u00e1ln\u00ed dr\u00e1hy BMP\" a \"transdukce sign\u00e1lu protein\u016f SMAD\",\" nazna\u010dil Otte.<\/p>\n\n\n\n

Auto\u0159i p\u0159izn\u00e1vaj\u00ed, \u017ee \"anal\u00fdza GO-term t\u00e9to sady gen\u016f p\u0159inesla jen n\u011bkolik m\u00e1lo v\u00fdznamn\u00fdch v\u00fdsledk\u016f kv\u016fli n\u00edzk\u00e9mu po\u010dtu b\u011b\u017en\u011b regulovan\u00fdch gen\u016f. V\u011bt\u0161ina t\u011bchto GO-term\u016f pro stav CSC-medium byla spojena s aktivn\u00ed transkripc\u00ed, bun\u011b\u010dnou proliferac\u00ed nebo zv\u00fd\u0161en\u00fdm p\u0159\u00edjmem gluk\u00f3zy. <\/p>\n\n\n\n

Po inhibici FGFR jsme na\u0161li regul\u00e1tory bun\u011b\u010dn\u00e9ho cyklu a mnoho gen\u016f anotovan\u00fdch jako \"diferenciace epiteli\u00e1ln\u00edch bun\u011bk\", \"tr\u00e1ven\u00ed\" nebo \"transport \u017elu\u010dov\u00fdch kyselin a \u017elu\u010dov\u00fdch sol\u00ed\".\"<\/p>\n\n\n\n

Dokumenty Dopad a v\u00fdkon<\/strong><\/h2>\n\n\n\n

M\u016f\u017eeme analyzovat pomoc\u00ed Altmetric<\/a> \u00fadaje, \u017ee sk\u00f3re online pozornosti tohoto \u010dl\u00e1nku je vy\u0161\u0161\u00ed. ne\u017e 68%<\/strong><\/strong> v\u0161ech ostatn\u00edch \u010dl\u00e1nk\u016f podobn\u00e9ho st\u00e1\u0159\u00ed v \u010dasopise Scientific Reports.<\/strong><\/p>\n\n\n\n

<\/div>\n\n\n
\n
\"\"<\/a>
Zdroj: Altmetrika<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

Zlep\u0161ete p\u0159\u00edstupnost sv\u00e9ho \u010dl\u00e1nku tak\u00e9 pomoc\u00ed infografiky.<\/p>\n\n\n\n

\n
Vyzkou\u0161ejte Mind the Graph<\/strong><\/a><\/div>\n<\/div>\n\n\n\n
<\/div>","protected":false},"excerpt":{"rendered":"

Inhibic\u00ed FGFR u pacientsk\u00fdch bun\u011bk CRC lze sn\u00e1ze pochopit tvorbu posti\u017een\u00fdch sf\u00e9roid\u016f a organoid\u016f.<\/p>","protected":false},"author":29,"featured_media":20014,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[195,958,957,1],"tags":[96,554,775,963],"yoast_head":"\nFGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog<\/title>\n<meta name=\"description\" content=\"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mindthegraph.com\/blog\/cs\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/\" \/>\n<meta property=\"og:locale\" content=\"cs_CZ\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog\" \/>\n<meta property=\"og:description\" content=\"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/mindthegraph.com\/blog\/cs\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/\" \/>\n<meta property=\"og:site_name\" content=\"Mind the Graph Blog\" \/>\n<meta property=\"article:published_time\" content=\"2022-09-10T21:27:00+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2023-03-17T17:47:45+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2022\/09\/blog_4.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1125\" \/>\n\t<meta property=\"og:image:height\" content=\"614\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Artur Zignani\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:image\" content=\"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2022\/09\/blog_4.jpg\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Artur Zignani\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"3 minutes\" \/>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog","description":"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/mindthegraph.com\/blog\/cs\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/","og_locale":"cs_CZ","og_type":"article","og_title":"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog","og_description":"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.","og_url":"https:\/\/mindthegraph.com\/blog\/cs\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/","og_site_name":"Mind the Graph Blog","article_published_time":"2022-09-10T21:27:00+00:00","article_modified_time":"2023-03-17T17:47:45+00:00","og_image":[{"width":1125,"height":614,"url":"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2022\/09\/blog_4.jpg","type":"image\/jpeg"}],"author":"Artur Zignani","twitter_card":"summary_large_image","twitter_image":"https:\/\/mindthegraph.com\/blog\/wp-content\/uploads\/2022\/09\/blog_4.jpg","twitter_misc":{"Written by":"Artur Zignani","Est. reading time":"3 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/mindthegraph.com\/blog\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/","url":"https:\/\/mindthegraph.com\/blog\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/","name":"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review - Mind the Graph Blog","isPartOf":{"@id":"https:\/\/mindthegraph.com\/blog\/#website"},"datePublished":"2022-09-10T21:27:00+00:00","dateModified":"2023-03-17T17:47:45+00:00","author":{"@id":"https:\/\/mindthegraph.com\/blog\/#\/schema\/person\/b44995c1d77b52a63b6d11262b740b02"},"description":"The Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells are easier to understand with our illustrations.","breadcrumb":{"@id":"https:\/\/mindthegraph.com\/blog\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/#breadcrumb"},"inLanguage":"cs-CZ","potentialAction":[{"@type":"ReadAction","target":["https:\/\/mindthegraph.com\/blog\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/mindthegraph.com\/blog\/success-case-fgf-signalling-in-the-self-renewal-of-colon-cancer-organoids\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/mindthegraph.com\/blog\/"},{"@type":"ListItem","position":2,"name":"FGF Signalling in the Self-Renewal of Colon Cancer Organoids Paper Review"}]},{"@type":"WebSite","@id":"https:\/\/mindthegraph.com\/blog\/#website","url":"https:\/\/mindthegraph.com\/blog\/","name":"Mind the Graph Blog","description":"Your science can be beautiful!","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/mindthegraph.com\/blog\/?s={search_term_string}"},"query-input":"required name=search_term_string"}],"inLanguage":"cs-CZ"},{"@type":"Person","@id":"https:\/\/mindthegraph.com\/blog\/#\/schema\/person\/b44995c1d77b52a63b6d11262b740b02","name":"Artur Zignani","image":{"@type":"ImageObject","inLanguage":"cs-CZ","@id":"https:\/\/mindthegraph.com\/blog\/#\/schema\/person\/image\/","url":"https:\/\/secure.gravatar.com\/avatar\/a211ec97011fe24f7a8e63a7ca799342?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/a211ec97011fe24f7a8e63a7ca799342?s=96&d=mm&r=g","caption":"Artur Zignani"},"url":"https:\/\/mindthegraph.com\/blog\/cs\/author\/artur\/"}]}},"_links":{"self":[{"href":"https:\/\/mindthegraph.com\/blog\/cs\/wp-json\/wp\/v2\/posts\/16636"}],"collection":[{"href":"https:\/\/mindthegraph.com\/blog\/cs\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/mindthegraph.com\/blog\/cs\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/mindthegraph.com\/blog\/cs\/wp-json\/wp\/v2\/users\/29"}],"replies":[{"embeddable":true,"href":"https:\/\/mindthegraph.com\/blog\/cs\/wp-json\/wp\/v2\/comments?post=16636"}],"version-history":[{"count":5,"href":"https:\/\/mindthegraph.com\/blog\/cs\/wp-json\/wp\/v2\/posts\/16636\/revisions"}],"predecessor-version":[{"id":27203,"href":"https:\/\/mindthegraph.com\/blog\/cs\/wp-json\/wp\/v2\/posts\/16636\/revisions\/27203"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/mindthegraph.com\/blog\/cs\/wp-json\/wp\/v2\/media\/20014"}],"wp:attachment":[{"href":"https:\/\/mindthegraph.com\/blog\/cs\/wp-json\/wp\/v2\/media?parent=16636"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/mindthegraph.com\/blog\/cs\/wp-json\/wp\/v2\/categories?post=16636"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/mindthegraph.com\/blog\/cs\/wp-json\/wp\/v2\/tags?post=16636"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}