{"id":16630,"date":"2022-09-08T18:14:00","date_gmt":"2022-09-08T21:14:00","guid":{"rendered":"https:\/\/mindthegraph.com\/blog\/success-case-zika-virus-replicates-in-adult-human-brain-tissue-and-impairs-synapses-and-memory-in-mice-copy\/"},"modified":"2023-03-17T14:47:54","modified_gmt":"2023-03-17T17:47:54","slug":"success-case-igh-resolution-synchrotron-based-x-ray-microtomography-as-a-tool-to-unveil-the-three-dimensional-neuronal-architecture-of-the-brain","status":"publish","type":"post","link":"https:\/\/mindthegraph.com\/blog\/cs\/pripad-uspechu-rentgenova-mikrotomografie-s-vysokym-rozlisenim-na-bazi-synchrotronu-jako-nastroj-k-odhaleni-trojrozmerne-neuronalni-architektury-mozku\/","title":{"rendered":"Synchrotronov\u00e1 rentgenov\u00e1 mikrotomografie s vysok\u00fdm rozli\u0161en\u00edm jako n\u00e1stroj pro odhalen\u00ed trojrozm\u011brn\u00e9 neuron\u00e1ln\u00ed architektury mozku Paper Review"},"content":{"rendered":"

Rentgenov\u00e1 mikrotomografie se stala novou metodou pro de\u0161ifrov\u00e1n\u00ed cytoarchitektury a konektivity mozku a my zde p\u0159edstavujeme metodu pro zobrazov\u00e1n\u00ed cel\u00fdch neuron\u016f.<\/p>\n\n\n\n

Synchrotronov\u00e1 rentgenov\u00e1 mikrotomografie s vysok\u00fdm rozli\u0161en\u00edm jako n\u00e1stroj k odhalen\u00ed trojrozm\u011brn\u00e9 neuron\u00e1ln\u00ed architektury mozku<\/strong><\/h2>\n\n\n\n

Matheus de Castro Fonseca et al. (2018) v \u010dl\u00e1nku \"High-resolution synchrotron-based X-ray microtomography as a tool to unveil the three-dimensional neuronal architecture of the brain\" (Rentgenov\u00e1 mikrotomografie s vysok\u00fdm rozli\u0161en\u00edm jako n\u00e1stroj k odhalen\u00ed trojrozm\u011brn\u00e9 neuron\u00e1ln\u00ed architektury mozku) uvedli, \u017ee studium neuron\u016f jako individualizovan\u00fdch jednotek m\u00e1 sv\u016fj po\u010d\u00e1tek v 19. stolet\u00ed, kdy byla vytvo\u0159ena doktr\u00edna neuron\u016f. <\/p>\n\n\n\n

V posledn\u00edch n\u011bkolika letech bylo vynalo\u017eeno velk\u00e9 \u00fasil\u00ed na pochopen\u00ed neuron\u00e1ln\u00ed organizace a konektivity v intaktn\u00edch vzorc\u00edch mozku. Technika zna\u010den\u00ed podle Golgiho-Coxe nab\u00edz\u00ed vhodn\u00fd kontrast pro specifick\u00e9 zna\u010den\u00ed neuron\u016f pro rentgenovou mikrotomografii. <\/p>\n\n\n\n

Neurony maj\u00ed odli\u0161nou morfologii a specifick\u00e9 vzorce propojen\u00ed, kter\u00e9 jsou nezbytn\u00e9 pro jejich spr\u00e1vn\u00e9 fungov\u00e1n\u00ed. V\u011bdci p\u0159edstavuj\u00ed metodu zobrazov\u00e1n\u00ed kombinuj\u00edc\u00ed rentgenovou mikrotomografii na synchrotronu s Golgiho-Coxov\u00fdm impregna\u010dn\u00edm protokolem. <\/p>\n\n\n\n

Metoda nab\u00edz\u00ed vy\u0161\u0161\u00ed a homogenn\u011bj\u0161\u00ed kontrast relativn\u011b \u0159\u00eddce rozm\u00edst\u011bn\u00fdch cel\u00fdch neuron\u016f v tk\u00e1ni.<\/p>\n\n\n\n

Mozky byly inkubov\u00e1ny v barvic\u00edm roztoku Golgiho-Coxe po dobu 14 dn\u016f a chr\u00e1n\u011bny p\u0159ed sv\u011btlem. Po uplynut\u00ed inkuba\u010dn\u00ed doby byly mozky s\u00e9riov\u011b roz\u0159ez\u00e1ny pomoc\u00ed vibratomu.<\/strong><\/p>\n\n\n\n

Zkontrolujte p\u016fvodn\u00ed \u010dl\u00e1nek zde<\/a>.<\/p>\n\n\n\n

Grafick\u00e9 abstraktum poh\u00e1n\u011bn\u00e9 Mind the Graph<\/strong><\/h2>\n\n\n\n
<\/div>\n\n\n
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\"\"<\/a>
Vyrobeno s Mind the Graph<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

Autor pou\u017eil Mind the Graph<\/a> k vytvo\u0159en\u00ed schematick\u00e9ho obr\u00e1zku, kter\u00fd zn\u00e1zor\u0148uje dosp\u011bl\u00e9 my\u0161i, kter\u00fdm je i.p. aplikov\u00e1n fyziologick\u00fd roztok nebo roztok pilokarpinu (280 mg\/kg). Zobrazen\u00ed slo\u017eit\u00fdch proces\u016f pomoc\u00ed infografiky je dobr\u00fdm zp\u016fsobem, jak je u\u010dinit snadno pochopiteln\u00fdmi, ani\u017e by byly opomenuty detaily a z\u00e1sadn\u00ed informace.<\/p>\n\n\n\n

Zd\u00e1 se, \u017ee n\u011bkter\u00e9 v\u00fdsledky v\u00fdzkumn\u00edk\u016f upev\u0148uj\u00ed to, co bylo o t\u00e9to oblasti zn\u00e1mo ji\u017e d\u0159\u00edve: \"Impregnace neuron\u016f na b\u00e1zi rtuti, kterou jsme zde uk\u00e1zali, n\u00e1m umo\u017enila jasn\u011b definovat cel\u00e9 neurony p\u0159edev\u0161\u00edm d\u00edky souvislej\u0161\u00ed a homogenn\u011bj\u0161\u00ed impregnaci bun\u011bk a mnohem men\u0161\u00ed hustot\u011b artefakt\u016f,\" nazna\u010dil de Castro Fonseca.<\/p>\n\n\n\n

Dokumenty Dopad a v\u00fdkon<\/strong><\/h2>\n\n\n\n

M\u016f\u017eeme analyzovat pomoc\u00ed Altmetric<\/a> \u00fadaje, \u017ee sk\u00f3re online pozornosti tohoto \u010dl\u00e1nku je vy\u0161\u0161\u00ed. ne\u017e 64%<\/strong><\/strong> v\u0161ech ostatn\u00edch \u010dl\u00e1nk\u016f podobn\u00e9ho st\u00e1\u0159\u00ed v \u010dasopise Scientific Reports.<\/strong><\/p>\n\n\n\n

<\/div>\n\n\n
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Zdroj: Altmetrika<\/a><\/em><\/figcaption><\/figure><\/div>\n\n\n

Zlep\u0161ete p\u0159\u00edstupnost sv\u00e9ho \u010dl\u00e1nku tak\u00e9 pomoc\u00ed infografiky.<\/p>\n\n\n\n

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Vyzkou\u0161ejte Mind the Graph<\/strong><\/a><\/div>\n<\/div>\n\n\n\n
<\/div>","protected":false},"excerpt":{"rendered":"

Pod\u00edvejte se na novou metodu de\u0161ifrov\u00e1n\u00ed cytoarchitektury a konektivity mozku zn\u00e1zorn\u011bnou pomoc\u00ed ilustrac\u00ed Mind the Graph.<\/p>","protected":false},"author":29,"featured_media":20013,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[195,958,957,960,1],"tags":[96,554,775,963],"yoast_head":"\nHigh-resolution synchrotron-based X-ray microtomography as a tool to unveil the three-dimensional neuronal architecture of the brain Paper Review - Mind the Graph Blog<\/title>\n<meta name=\"description\" content=\"Look into the new method for deciphering the cytoarchitecture and connectivity of the brain represented with Mind the Graph's illustrations.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mindthegraph.com\/blog\/cs\/pripad-uspechu-rentgenova-mikrotomografie-s-vysokym-rozlisenim-na-bazi-synchrotronu-jako-nastroj-k-odhaleni-trojrozmerne-neuronalni-architektury-mozku\/\" \/>\n<meta property=\"og:locale\" content=\"cs_CZ\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"High-resolution synchrotron-based X-ray microtomography as a tool to unveil the three-dimensional neuronal architecture of the brain Paper Review - 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